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Department of Cryobiochemistry
Research fellows:

The department was created in 1972 under the leadership of corresponding member of National Academy of Sciences of the Ukraine, Apollon M. Belous (1929-1999), who was the head of scientific school and direction: cryobiochemistry of membrane structures of cells as well. Since 1999 the head of department is winner of the Prize of National Academy of Sciences of Ukraine in the field of biochemistry and molecular biology named by O.V. Palladin (1999), winner of State prize of Ukraine in the field of science and technology (2002) Prof. Alexandr Yu. Petrenko, DSc (Biol)

Head of the Department
Alexandr Yu. Petrenko
Prof., DSc (Biol)

Under his leadership the department has continued to actively study the mechanisms of cryodamage, cryoprotection and reparation of cell cultures and subcellular membranes, by deepening and widening the research area. More >>

An important direction of the Department activity is the development of the strategy of hypothermic storage of organs. At the Department there has been designed the medium for hypothermic storage, which on its efficiency does not differ from current "golden" standard: UW Solution, but it is quite more simple and cheap than latter. More >>

The leading direction of the Department activity is isolation, cryopreservation and investigation of stem cells. Therapeutic use of stem cells now is hopeful for treatment of oncological, haematological, neurological diseases. Big perspectives are laid to the creation of new organs with their help. Therefore fundamental researches of stem cells directed to the studying of their cryobiological characteristics, revealing of the mechanisms of propagating in an organism during transplantation, self-renewal, regulation of differentiation and apoptosis in different model systems are the objects of the Department researches. More >>

Embryonic tissue is the richest source of regional stem cells. The most valuable organ in this respect is liver, which at early stages of an organism development is the site of hemopoiesis and on the number of hemopoietic stem cells, their proliferative potential strongly exceeds bone marrow and cord blood. In addition to hemopoietic cells in embryonic liver there are mesenchymal and parenchymal stem cells, high differentiative potential of which enables to create new methods of treating some human diseases.

Stem cell research includes the studying of proliferative and differentiative potentials, cryobiological characteristics, possibilities of genetic modification and creation of cell lines with certain properties, effect mechanisms in various systems in vitro and in the models of pathological states in laboratory animals. More >>

Today the scientific interests of the Department comprise the studying of regional stem cells of mammalians and human being, including non-hemopoietic stem cells (mesenchymal, epithelial, hepatic), their phenotype, cryosensitivity, metabolic state of proliferative and differentiative potential under culturing conditions. More >>

The effect of transplanted cells is realised by a complicated cascade of biochemical regulatory reactions, one of this effect mechanisms is the modulation of lipid peroxidation intensity. Nowadays at the Department the role of some embryo specific factors in realisation of therapeutic effect of transplanted cells is under study.

Basing on fundamental studies in biology and cryobiology of stem cells at the Department there have been developed the technologies of creating some cell preparations to be used in clinics of Ukraine and abroad. Investigations and clinical trials of these preparations testify to a high efficiency of their application in medicine. In particular, cryopreserved HEMOCELLS, cells of human embryonic liver, containing hemopoietic, hepatic and mesenchymal stem cells, find a wide application in haematology, oncology, hepatology, urology, ophthalmology [16], in traumatology and orthopaedia [17].

Transplantation of "NEUROCELLS" preparation, enriched with multipotent neuronal stem cells, is one of attractive method of substituting anatomic or functional defects of central and peripheric nervous system. More >>

It has been established that for some pathologies, in particular, eye diseases, it is expedient to apply hemopoietic and nerve stem cells. In this connection there was developed the "HEMONEURONAL" preparation, containing under certain proportions cryopreserved embryonic cells of liver and human brain [20-21].

The development of the method of cell isolation of mesenchymal-mesodermal origin derived from human embryos of 4-12 weeks of gestation ("MESOCELLS") [22] enabled to receive the suspension, representing the cocktail of the cells of various genesis with prevailing fibroblast-like ones of a high proliferative activity. This suspension is characterised with a high content of stem cells, primarily mesenchymal ones, and possess a high colony-forming activity in culture. The cryopreservation method of these cells has been developed. Transplantation of cryopreserved mesenchymal cells to laboratory animals with model pathologies results in a rapid healing of burns and wounds, recovery of moving function after joints' damage [23]. Clinical trials have shown their high efficiency for curing the burns, rheumatoid arthritis, arthrosis of big joints [24-25]. Those who developed the preparation of mesenchymal cells cherish the hopes for their clinical application in cardiology, traumatology, gastroenterology, as well as emphasise their prospects in creation of artificial skin and other tissues and organs.

Fundamental studies of the Department in the field of biochemistry, cell biology, cryobiology, hypothermic storage of organs and experimental transplantation are directed to the needs in contemporary medicine and are performed together with leading Ukrainian and foreign researchers under support of Wellcome Trust and INTAS. The Department staff is keen of extending and tightening these relations and ready to performing mutual projects with the interested scientists of various fields of experimental and clinical medicine.

23, Pereyaslavskaya str., Kharkov, Ukraine 61016
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
Tel: +380 57 373 4143,   373 3807,   373 3039;     Fax: +380 57 373 3084
E-mail: cryo@online.kharkov.ua