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Department of Cryobiophysics
Scientific Fellows of the Department:

The department was established at the Institute in 1976. From the day of establishment of Department till 2000 it was headed by Prof., Dr. Moyiseev V.A., from 2000 till now the Department is headed by Dr. Nardid O.A..

Head of the Department:
Oleg A. Nardid
DSc (Biol)

Such methodical approaches as radiospectroscopic methods (electron magnetic resonance, nuclear magnetic resonance), spectroscopy in a visible, ultraviolet and infrared areas, spectrofluorimetry, small angle x-ray scattering, microcalorimetry within a wide temperature range are used for scientific investigations at the department.

The main scientific task of the fundamental plan to be solved by the department is known to be the understanding the molecular mechanisms for cryodamage and cryoprotection of biological systems of different organizational level. The major trends of scientific studies performed at the department are as follows:

  1. Studying the structure of aqueous-cryoprotective solutions, cryoprotectant interaction with biomacromolecules and membranes, effect on metabolism of macroergic phosphorus containing compounds, possibilities of ozone technologies in cryobiology (Prof. V.D.Zinchenko, DSc (Biol));

  2. Studying intermolecular interactions in aqueous-cryoprotective salines aimed to prevent the cryodamages by salt and metabolite hyperconcentrations, effect of different cryopreservation stages on a cytosol state and damage of membrane barrier function in erythrocytes, hepatocytes, mitochondria, lisosomes (Nardid O.A., PhD (Phys&Math));

  1. Studying the phase state of aqueous-cryoprotective mixtures, mechanical and electrical effects at metastable states appearing during the process of cooling and heating of cryobiological systems, as well as those which comprise the bioobjects (Zinchenko A.V., DSc (Biol));

  2. Isolation and studying the series of native proteins being both in an isolated state and as proteolyposomes, as well as the effect on their structural and functional state of freezing regimens, storage, salts and cryoprotectants (Rozanova K.D., PhD (Biol));

  3. Studying the effect of low temperatures, salts and cryoproetctants on the dynamic state of cytoplasmatic membranes, as well as the interaction between membrane and cytosol components (Tsymbal L.V., PhD (Biol));

  4. Studying at the molecular level of natural mechanisms for mammalian adaptation to low temperatures, determining the role in this process of plasmatic membrane and cytoskeleton (Repina S.V., PhD (Biol));

  5. Studying the cell membrane permeability for cryoprotective substances, their redistribution and interaction with membrane, use of biologically active substances derived from biological tissues of mammals in clinical practice (Schetinsky M.I., PhD (Biol)).

Department of Cryobiophysics holds tight contacts with leading scientific European Centers, Dr. Kucherenko Yu.V. worked at the University of Saarland, Laboratory of Biophysics, Saarbrueken, Germany and University of Cambridge, Department of Clinical Veterinary Medicine, UK. Most of the methodical approaches used for research at the Department are original and elaborated at the Institute with the copyright patented. All the equipment being used here is designed and produced at the Institute and its Special Designing Technical Bureau with Experimental Unit.

23, Pereyaslavskaya str., Kharkov, Ukraine 61016
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
Tel: +380 57 373 4143,   373 3807,   373 3039;     Fax: +380 57 373 3084
E-mail: cryo@online.kharkov.ua