There was found out the relationship between the character of PEO-1500 binding with erythrocyte membranes at the incubation stage, a change in their volume and shape, modification of cytoskeletal proteins, state of membrane barrier properties and erythrocyte resistance to freeze-thawing .
Under conditions of incubations of cells at 0°C, when a decrease in cryoprotectant binding with cells is observed, there are occurred structural and functional rearrangements of plasmatic membranes and cytoskeletal proteins, resulting in erythrocyte volume and shape "fixing" and providing in many respects the preservation of membrane barrier properties, that finally contributes to the augmentation of erythrocyte resistance to cryopreservation.
As a result of conducted study of cell stabilisation mechanisms to the effect of a non-penetrating cryoprotectant and low temperatures there were substantiated the new approaches for a targeted modification of structural and functional cell state on the initial stages before cryopreservation with the aim to increase their resistance to freeze-thawing .These approaches are in the formation of the definite temperature-osmotic conditions, under which the stabilisation of plasmatic membranes and cytoskeletal proteins occurs. Basing on the principle of "cold stabilisation" the without washing-out way for erythrocyte cryopreservation, allowing in a great extent to increase their resistance to freeze-thawing and to preserve structural and functional cell indices at a quite high level, was developed and patented in Ukraine . This method is more perspective, comparing to the existing ones and can get more wide usage in practical medicine .
Nowadays a special attention is paid to the investigation of human cord blood. We investigate the influence of cryoprotectants with various effect mechanisms and cryopreservation process on erythrocytes and other cord blood components . The structural and functional integrity of cord blood cells after cryopreservation has been determined. Cryopreservation method without washing-out for the whole human cord blood preparation to be used in clinical practice has been developed.