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Department of Cryopathophysiology
Research Fellows:

Department was established in 1993. Department is headed by Professor, DSc (Med), corresponding-member of National Academy of Sciences of Ukraine Anatoly N. Goltsev. Up to now there have been performed the investigations of great theoretical and practical importance for cryobiology and cryomedicine. There has been developed an alternative to cryopreservation variant of minimisation of bone marrow immune reactivity when introducing the products of embryofetoplacental complex (PEFPC), in particular embryonic liver cells (ELCs) and placenta suspension (PS), which have the capability not only to reduce the GVHR-activity of myelotransplant, but to increase its protective potential as well.

Head of the Department
Anatoliy N. Goltsev
Corr. Memb. of the National
Academy of Sciences
of Ukraine
Prof., DSc (Med)

There were elaborated the methods for ELCs, placenta and cord blood cryopreservation that permitted a successful usage of cryopreserved PEFPC in clinical practice when treating hemato- and immunodeficient states of an organism.

Since 1997 and till now the study of pathophysiological grounds of autoimmune diseases (AID) different forms development and their treatment using the cryopreserved and native PEFPC has been the main direction. The effect mechanism decoding of introduced material is carried-out within a wide range of different experimental models of AID development (autoimmune hemolytic anemia: AIHA, adjuvant arthritis: AA and experimental allergic encephalomyelitis: EAE), that allows to reveal both general regularities of preparation effect and fundamental differences.

In the AIHA model the PS and ELCs application is accompanied by an increase in autoerythrocyte antibodies titer, as well as by a decrease in number of animals with a positive Coombs' reaction. In AA model the PS application caused a decrease in paw oedema and arthritis index at the stage of a generalised development in pathology. For the first time the effect of apoptotic process stimulation using PS was obtained in AA model. In EAE model it was noted, that a pathogenetically significant vector for this pathology treatment might be the embryonic neuronal cells (ENCs) application. The manifestation of different degree of ENCs activity was established as might be determined by the phase of immunoinflammatory reaction development, which under certain terms could have more favourable profile for such therapy performance.

Of special importance are the results about the appearance of more manifested positive effect of PEFPC, cryopreserved with certain regimens, which under certain doses and introduction protocols can correct an immune competent sphere state under pathology development. Owing to these works the authors demonstrated the cryopreservation capability as being not only the method for a long-term storage of cell and tissue-organ structures, but the factor for bioobject intrinsic state controlling as well. These investigations are patented and upon the work results the methodical recommendations were published.

Recently the collaborators of Department of Cryopathophysiology together with Kavetsky Institute have studied the mechanisms of PEFPC effect on a lymphohemopoietic system of animals with genetically determined expansion of tumor process.There were obtained the data about the fact, that the PEFPC correcting activity was manifested in different terms of tumor process development in a variable extent. This fact determines the necessity for further study and substantiation of programs for their therapeutic application.

Since 2002 the Pathophysiological Department together with the colleagues of Department for Cryobiology of Reproductive System have started more profound investigation on molecular level of PEFPC effect on genome of immunocompetent cells and the modification degree of their intercellular cooperation during AID development.

23, Pereyaslavskaya str., Kharkov, Ukraine 61016
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
Tel: +380 57 373 4143,   373 3807,   373 3039;     Fax: +380 57 373 3084
E-mail: cryo@online.kharkov.ua