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Department of Cryobiochemistry
and Pharmacology
of Neurohumoral Systems
Research Fellows:

Department is headed by Prof. Tatyana P. Bondarenko, DSc (Biol). Successful application of cell and tissue transplantation to treat endocrinopathies resulted in the necessity to elaborate the methods for long-term storage of transplantational material.

Head of the Department:
Tatyana P. Bondarenko
DSc (Biol), Prof.

In this connection the main direction of scientific work in the department is the development of methods for endocrine tissue organ culture cryopreservation. The result of the works performed was the establishing of the low temperature bank for organ cultures of adrenocortical tissue, those of thyroid gland and testicles.

In addition, proceeding from modern scientific notions about cryopreservation as not only the way for a long-term storage of transplantational material, but as the factor likely affecting a transplant's immunogenity as well, the department carries-out the works on studying the functioning of allo- and xenografts after transplantation.

Along with an experimental scientific activity the works on introducing the obtained results into a clinical practice are performed at the department. Transplantation of cryopreserved organ cultures of endocrine tissues was successfully approved when treating the patients with hypocorticoidism, hormone-dependent bronchial asthma, idiopathic and post-operative hypothyreosis and some other diseases. .

Characteristics and indices for usage of endocrine glands' organ cultures:

1. Organ culture of adrenal glands. Organ culture of adrenal glands (cryopreserved) comprises the cells of cortical and medullar zones of adrenal glands and has hormone-synthesising and hormone-secreting capabilities of native gland. The range of steroid hormones, produced by a transplant compensates and recovers in the most physiological way the lost functions of adrenal glands themselves, entering into a closed cycle of endocrine system hormonal regulation by allowing to reduce the dose or to cancel quite completely the used earlier synthetic glucocorticoids.

Indications for usage. Different forms of chronic adrenal insufficiency, as well as the diseases and pathological states when the necessity occurs to apply glucocorticoid hormones. Average severe and severe forms of Addison's disease at the state of clinical compensation or subcompensation, non-specific polyarthritis, allergies, autoimmune destruction of adrenal cortex, destruction, caused by tuberculosis, amyloidosis, fungal infections. Bronchoobstructive pulmonary diseases with hormonal dependence, non-differentiating remission, a slight effect of glucocorticoids. Medicamentous hypocorticoidism. Iatrogenic adrenal insufficiency after: a) bilateral adrenalectomy because of Icenko-Kushing disease; b) bilateral tumors of adrenal gland and chromaffin tissue; c) malignant essential hypertension; d) bilateral haemorrhage into adrenal gland at the background of therapy with anticoagulants. .

2. Thyroid gland organ culture. Thyroid gland organ culture after cryopreservation cycle preserves a follicular structure, identic to native tissue and the capability to thyroid hormone synthesis de novo. An organ culture keeps also the capability to an adequate response to TTH-stimulation. The thyroid hormones (thyroxin, triiodothyronine), produced in a microtransplantation site compensate in most physiological way the lost functions of own thyroid gland. A transplant enters into a closed cycle of endocrine system hormonal regulation by a hypothalamo-hypophysial effector axis, allows to reduce the dose or to quite completely cancel the earlier used hormonal preparations.

Indication for usage. Average and severe hypothyrosis forms of different etiology, requiring a constant hormonal substitutive therapy. Individual intolerance for hormonal preparations.

Recent publications

23, Pereyaslavskaya str., Kharkov, Ukraine 61016
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
Tel: +380 57 373 4143,   373 3807,   373 3039;     Fax: +380 57 373 3084
E-mail: cryo@online.kharkov.ua