Effect of Cryopreservation According Two-Stage Program in High-Molecular Dextran Solutions on Cytomorphological and Functional Properties of Human Cord Blood Cells

Authors

  • Olga Yu. Kozhina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Maksim V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Igor G. Grisha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Nikolay A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

human cord blood, nucleated cells of blood, cryopreservation, high-molecular dextran, two stage freezing

Abstract

Human cord blood (HCB) is a conventional source of stem cells and biologically active substances. Long-term storage of this unique biomaterial is possible under conditions of low temperature bank. In this research we studied the effect of cryopreservation according to two-stage freezing program on cytomorphological and functional characteristics of cells of HCB leukoconcentrate (HCBL). It was shown that after cryopreservation under protection of high molecular dextran, redistribution in cell composition of HCBL occurred due to reduction of granulocytes population, increase of non-differentiated cells and macrophages number. Quite a high resistance to effect of cryopreservation factors had stem and early progenitor cells. There was noted a reduction of total bactericidal activity of HCBL cells and increase of phagocytic activity of macrophages. Probl Cryobiol Cryomed 2013; 23(1):58–65.

Author Biographies

Olga Yu. Kozhina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Maksim V. Ostankov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Igor G. Grisha, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Nikolay A. Bondarovich, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

References

Actual problems of pathophysiology: selected lectures. Study guide for students of medical universities. Ed. by B.B. Moroz Moscow: Meditsyna, 2001.

Aleksandrov M.G., Kudryavitsky A.I., Rumyantseva E.G. et al. Determination method of absolute indices of phagocytosis. Laboratornoe Delo 1988; (90: 30–32.

Boyko V.V., Grischenko V.I., Krivoruchko I.A. et al. Application of cord blood in patients with gastric hemorrhage of ulcerative genesis. Ukr Zhurnal Maloinvazivnoi ta Endoskopichnoi Khirurgii 2001; 5(1): 11–12.

Goltsev A.N., Kalinichenko T.A. Umbilical human cord blood as a source of hemopoietic cells for clinical application. Part 2. Immunological characteristics. Problems of Cryobiology 1988; (1): 3–24.

Goltsev K.A., Krivoruchko I.A., Achgibesov K.A. et al. Application of cryopreserved cord blood in complex therapy of acute pyoperitonitis in rats. Meditsyna Segodnya i Zavtra 2011; (1–2): 24–30.

Grischenko V.I., Goltsev A.N. Modification of state of lympho-hemopoietic complex of an organism using products of fetopla-cental complex. Transplantologiya 2001; (3): 5.

Lakin G.F. Biometry Moscow: Vysshaya shkola, 1980.

Lobyntseva G.S. Theoretical tasks of stem cell cryopreservation. Operating peculiarities of umbilical blood cryobank. Transplantologiya 2007; 3 (1): 104.

Microscopic technique: Manual for doctors and laboratory assistants / Ed. by D.S. Sarkisova and Yu.A. Perova Moscow: Meditsyna, 1996.

Plyasunova S.A. Cell composition of umbilical blood of full-term newborns [Abstract of the Thesis of Candidate of Medical Sciences]. Moscow, 2006.

Raydan M. Increased resistance of non-differentiated and cancer cells to impairing effect of low temperatures [Abstract of the Thesis of Candidate of Biological Sciences]. St. Petersburg, 2011 26 p.

Tsutsayeva A.A., Kudokotseva O.V., Scheglov A.V. et al. Cord blood as a component of maintenance therapy. Problems of Cryobiology 2001; (3): 93.

Tsutsayeva A.A., Grischenko V.I., Prokopyuk O.S. et al. Washing-free method of cryopreservation of hemopoietic cells from human cord blood for clinical application [Guidelines]. Kharkov, 2000.

Tsutsayeva A.A., Glushko T.O., Lobasenko N.P. et al. Properties of cryopreserved transplants of stem hemopoietic cells. Transplantologiya 2004; (3): 374–377.

Tsutsayeva A.A., Tsyganenko A.Ya., Zheltyakova I.A. Influence of hypothermic storage before and after cryopreservation on properties of nucleated components and whole cord blood plasma. Problems of Cryobiology 2006; 16 (1): 45–55.

Tsymbalyuk V.I., Zhilka N.Ya., Kidon' V.P., Bakhantsova N.M. Umbilical blood: perspectives of stem cell application. Zdorov'ya Ukrainy 2007; (9): 74.

Patent of Ukraine N31847A IPC A01N1/02. Method of cryopreservation of cord blood hemopoietic cells. A.O. Tsutsayeva, V.I. Grischenko, O.V. Kudokotseva et al. Applied 05.11.98. Publ. 15.12.2000. Bull. N7: 1–10.

Berz D., McCormack E.M., Winer E.S. et al. Cryopreservation of hematopoietic stem cells. Am J Hematol 2007; 82 (6): 463–472.

Harner S., Noessner E., Nadas K. et al. Cord blood Vb24-Vb11+ natural killer T cells display a Th2-chemokine receptor profile and cytokine responses. PLoS One 2011; 6 (1): e15714.

Kadowaki N., Antonenko S., Ho S. et al. Distinct cytokine profiles of neonatal natural killer T cells after expansion with subsets of dendritic cells. J Exp Med 2001; 193 (10): 1221–1226.

Ketheesan N., Whiteman C., Malczewski A.B. et al. Effect of cryopreservation on the immunogenicity of umbilical cord blood cells. Transfus Apher Sci 2004; 30 (1): 47–54.

Lecoeur H. Nuclear apoptosis detection by flow cytometry: influence of endogenous endonucleases. Exp Cell Res 2002; 277 (1): 1–14.

Urbanitz D., Fechner T., Grob R. Nitroblau–Tetrazolium (NBT) Test bei isolierten menschlichen Monozyten. Blut 1975; 30 (3): 187–198.

Wachstein M.S. Histochemistry of leucocytes. Ann NY Acad Sci 1955; 59 (5): 1052–1065.

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Published

2013-03-15

How to Cite

Kozhina, O. Y., Ostankov, M. V., Grisha, I. G., & Bondarovich, N. A. (2013). Effect of Cryopreservation According Two-Stage Program in High-Molecular Dextran Solutions on Cytomorphological and Functional Properties of Human Cord Blood Cells. Problems of Cryobiology and Cryomedicine, 22(1), 58–65. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/10

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

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Copyright (c) 2020 Olga Yu. Kozhina, Maksim V. Ostankov, Igor G. Grisha, Nikolay A. Bondarovich

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