Cryopreservation of Newborn Mice Adrenal Cell Suspension: 1. Effect of DMSO Concentration as a Component of Cryoprotective Medium

Authors

  • I. V. Tamarina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. F. Gubina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, adrenal glands, newborn mice, DMSO

Abstract

Influence of DMSO concentrations as a component of cryopreservative medium on viability, survival and functional activity of the newborn mice adrenal cells after freeze-thawing cycle has been established in this study. Maximal values of viability (63.7; 66.7%) and survival (78.3; 95.7%) are observed after freezing of newborn mice adrenal cell suspension (ACS) in the medium with 15 and 20% DMSO. Regardless the high values of ACS viability and survival after freeze-thawing cycle, none of the DMSO concentrations being used was not capable to preserve proper post-thaw cell proliferation and hormone production.

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Published

2024-05-21

How to Cite

Tamarina, I. V., Bozhok, G. A., Gurina, T. M., Gubina, N. F., & Bondarenko, T. P. (2024). Cryopreservation of Newborn Mice Adrenal Cell Suspension: 1. Effect of DMSO Concentration as a Component of Cryoprotective Medium. Problems of Cryobiology and Cryomedicine, 22(1), 79–87. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/100

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 I. V. Tamarina, G. A. Bozhok, T. M. Gurina, N. F. Gubina, T. P. Bondarenko

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