DMSO Effect on Phase Transitions and Vitrification in Cord Blood Erythrocyte Suspension Under 0°C

Authors

  • A. V. Zinchenko
  • E. N. Bobrova
  • M. I. Schetinskiy

Keywords:

differential scanning calorimetry, NMR, DMSO, erythrocytes, cord blood

Abstract

Using the DSC method there were investigated the cord blood erythromass, mixed in 1:1 ratio with DMSO aqueous solutions of different concentrations, as well as the samples of dense sediment and supernatant fluid, obtained after these mixtures’ centrifugation. The temperatures of vitrification, devitrification, crystallisation and melting of eutectic compositions and the temperature of complete melting of systems were determined. There was built a part of diagram for physical states of water-DMSO binary system within the concentration range from 0 up to 60 % (w/w) of DMSO. Using the NMR method it was shown, that the DMSO concentration in supernatant fluid was higher, than in a dense suspension of erythrocytes. This explains the differences in eutectics crystallisation in the samples of erythrocyte dense suspension and in supernatant fluid.

Author Biographies

A. V. Zinchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

E. N. Bobrova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

M. I. Schetinskiy

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

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Published

2006-06-25

How to Cite

Zinchenko, A. V., Bobrova, E. N., & Schetinskiy, M. I. (2006). DMSO Effect on Phase Transitions and Vitrification in Cord Blood Erythrocyte Suspension Under 0°C. Problems of Cryobiology and Cryomedicine, (2), 16–21. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1026

Issue

No. 2 (2003): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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