Role of Forming Aggregates in Post-Thaw Survival of Isolated Neural Cells from Newborn Rats

Authors

  • A. N. Sukach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov G.S. Skovoroda Kharkov National Pedagogical University, Kharkov
  • T. D. Lyashenko G.S. Skovoroda Kharkov National Pedagogical University, Kharkov

Keywords:

neural cells of newborn rats, multicellular aggregates, survival, viability, cryopreservation, cultivation

Abstract

The role of formation of newborn rat neural cell aggregates on cell recovery after isolation and cryopreservation was studied. Short-term cultivation of neural cells within aggregates eliminate the damages that appear during cell isolation and possibly protects from apoptosis. It was shown that freezing of neural cells within aggregates under protection of 10% DMSO is effective for storage of stem/progenitor and differentiated neural cells. Maintenance of integrity of neural cell aggregates after cryopreservation is a necessary condition for survival in culture.

Downloads

Published

2011-12-19

How to Cite

Sukach, A. N., & Lyashenko, T. D. (2011). Role of Forming Aggregates in Post-Thaw Survival of Isolated Neural Cells from Newborn Rats. Problems of Cryobiology and Cryomedicine, 21(4), 395–406. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/111

Issue

Vol. 21 No. 4 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. N. Sukach, T. D. Lyashenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).