Cryopreservation of Newborn Mice Adrenal Cell Suspension. II. Effect Caused by Concentration of Serum as Component of Cryoprotective Medium

Authors

  • Irina V. Tamarina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Galina A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Tatyana M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Nina F. Gubina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Tatyana P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, adrenal glands, newborn mice, DMSO

Abstract

The effect of fetal bovine serum (FBS) and dimethylsulfoxide (DMSO) concentrations within cryoprotective medium on the viability indices by exclusion of trypan blue, survival and hormonal activity of adrenal cells of newborn mice after freeze-thawing has been studied. Application of 10 and 25% serum enabled to increase a number of viable adrenal cells of newborn rats if compared with serum-free media. The highest indices of viability (from 71.4 upto 76.0%) and survival (99.0%) were observed after freeze-thawing of adrenal cells in the media, containing 10, 15, 20% DMSO and 25% FBS. However, hormonal and proliferative activities were reduced if compared with the control culture. Probl Cryobiol Cryomed 2013; 23(1):66–74.

Author Biographies

Irina V. Tamarina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiochemistry and Pharmacology of Neurohumoral Systems

Galina A. Bozhok, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiochemistry and Pharmacology of Neurohumoral Systems

Tatyana M. Gurina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long Term Storage of Biological Objects at Low Temperatures and Microbiology

Nina F. Gubina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Tatyana P. Bondarenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiochemistry and Pharmacology of Neurohumoral Systems

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Published

2013-03-15

How to Cite

Tamarina, I. V., Bozhok, G. A., Gurina, T. M., Gubina, N. F., & Bondarenko, T. P. (2013). Cryopreservation of Newborn Mice Adrenal Cell Suspension. II. Effect Caused by Concentration of Serum as Component of Cryoprotective Medium. Problems of Cryobiology and Cryomedicine, 22(1), 66–74. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/12

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Section

Cryopreservation of Biological Resources