Effect of Cryopreserved Cord Blood Serum Administration on Structural Changes of Joints During Induced Gonarthrosis in Rats

Authors

  • Boris P. Vvedenskiy V.N. Karazin Kharkiv National University, Kharkiv Kharkiv Railway Clinical Hospital â„–2 of «Healthcare Center» Branch of the Ukrainian Railways PJSC, Kharkiv
  • Gennadiy A. Kovalev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Ninel V. Dedukh Sytenko Institute of Spine and Joint Pathology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Boris P. Sandomirskiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.04.361

Keywords:

cryopreserved cord blood serum, gonarthrosis, joint capsule, cartilage, chondrocytes

Abstract

In the paper we assessed the morphological changes of rat's knee joint tissues in arthrosis simulatiion and therapy with cryopreserved human cord blood serum. Gonarthrosis was simulated by a single injection into a joint cavity of 35° ethanol solution cooled down to –25°C. It has been found that in the control animals (untreated) at all the stages of monitoring the signs of gonarthrosis progressed. To day 7 in joint cavity there were found the tissue fragments containing detritus, and starting from day 14 the pannus formation was revealed. Intramuscular injection of either Alflutop drug (control group) or the cryopreserved human cord blood serum (experimental group) was accompanied by a positive dynamics of reparation, which was more pronounced when treating with cryopreserved human cord blood serum, but a complete restoration of an articular cartilage was not observed. Treatment with cryopreserved human cord blood serum was helpful in slowing down the degeneration of articular coating, preventing the pannus formation, it reduced the pathology activity in a joint capsule. More dense packing of erythrocytes in a cartilage was accompanied by restoration of their functional activity.

Probl Cryobiol Cryomed 2016; 26(4): 361–374

Author Biographies

Gennadiy A. Kovalev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Experimental Cryomedicine

Boris P. Sandomirskiy, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Experimental Cryomedicine

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Published

2016-12-23

How to Cite

Vvedenskiy, B. P., Kovalev, G. A., Dedukh, N. V., & Sandomirskiy, B. P. (2016). Effect of Cryopreserved Cord Blood Serum Administration on Structural Changes of Joints During Induced Gonarthrosis in Rats. Problems of Cryobiology and Cryomedicine, 26(4), 361–374. https://doi.org/10.15407/cryo26.04.361

Issue

Section

Cryomedicine, Clinical and Experimental Transplantology