Effect of Cryopreserved Cord Blood Serum Administration on Structural Changes of Joints During Induced Gonarthrosis in Rats


  • Boris P. Vvedenskiy V.N. Karazin Kharkiv National University, Kharkiv Kharkiv Railway Clinical Hospital â„–2 of «Healthcare Center» Branch of the Ukrainian Railways PJSC, Kharkiv
  • Gennadiy A. Kovalev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Ninel V. Dedukh Sytenko Institute of Spine and Joint Pathology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Boris P. Sandomirskiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv




cryopreserved cord blood serum, gonarthrosis, joint capsule, cartilage, chondrocytes


In the paper we assessed the morphological changes of rat's knee joint tissues in arthrosis simulatiion and therapy with cryopreserved human cord blood serum. Gonarthrosis was simulated by a single injection into a joint cavity of 35° ethanol solution cooled down to –25°C. It has been found that in the control animals (untreated) at all the stages of monitoring the signs of gonarthrosis progressed. To day 7 in joint cavity there were found the tissue fragments containing detritus, and starting from day 14 the pannus formation was revealed. Intramuscular injection of either Alflutop drug (control group) or the cryopreserved human cord blood serum (experimental group) was accompanied by a positive dynamics of reparation, which was more pronounced when treating with cryopreserved human cord blood serum, but a complete restoration of an articular cartilage was not observed. Treatment with cryopreserved human cord blood serum was helpful in slowing down the degeneration of articular coating, preventing the pannus formation, it reduced the pathology activity in a joint capsule. More dense packing of erythrocytes in a cartilage was accompanied by restoration of their functional activity.

Probl Cryobiol Cryomed 2016; 26(4): 361–374

Author Biographies

Gennadiy A. Kovalev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Experimental Cryomedicine

Boris P. Sandomirskiy, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Experimental Cryomedicine


Ainola M.M., Mandelin J.A., Liljestrom M.P. et al. Pannus invasion and cartilage degradation in rheumatoid arthritis: involvement of MMP-3 and interleukin-1beta. Clin Exp Rheumatol 2005; 23(5): 644–650. PubMed

Dhawan A., Mather R., Karas V. et al. An epidemiologic analysis of clinical practice guidelines for non-arthroplasty treatment of osteoarthritis of the knee. Arthroscopy 2014; 30(1): 65–71. CrossRef PubMed

Farr J., Miller L., Block J. Quality of life in patients with knee osteoarthritis: a commentary on nonsurgical and surgical treatments. Open Orthop J 2013; (7): 619–623.

Fransen M., Bridgett L., March L. et al. The epidemiology of osteoarthritis in Asia. Int J Rheum Dis 2011; 14(2): 113–121. CrossRef PubMed

Goldring M.B. Articular cartilage degradation in osteoarthritis. HSS Journal 2012; 8(1): 7–9. CrossRef PubMed

Goltsev A.N., Yurchenko T.N. Placenta: cryopreservation, clinical application. – Kharkiv, 2013.

Grischenko V.I., Kovalov G.A., Petrenko A.Yu. et al. Regenerative plastic therapy of alcohol visceropathies. – Kyiv: Naukova dumka, 2010. – 152 p.

Grischenko V.I., Prokopyuk O.S., Lipina O.V. Procurement, cryopreservation of serum and plasma of cord blood and their clinical use. Methodical recommendations. – Kharkiv, 2000. – 11 p.

Gul A., Lone K., Ahmad A. Implications of umbilical cord blood serum in clinical applications. The Health 2013; 4(4): 71–75.

Hafez A., Al-Johani A., Zakaria A. et al. Treatment of knee osteoarthritis in relation to hamstring and quadriceps strength. J Phys Ther Sci 2013; 25(11): 1401–1405. CrossRef PubMed

Haga P., Ek J., Kran S. et al. Plasma tocopherol levels and vitamin E/beta-lipoprotein relationships during pregnancy and in cord blood. Am J Clin Nutr 1982; 36(6): 1200–1204.

Heidari B. Knee osteoarthritis prevalence, risk factors, pathogenesis and features: Part I. Caspian J Intern Med 2011; 2(2): 205–212.

Hu D., Gu H., Cao L. et al. Study on the relationship between endogenous opioid peptides and fetal distress. Zhonghua Fu Chan Ke Za Zhi 2002; 37(12): 718–720.

Kanno H. Regenerative therapy for neuronal diseases with transplantation of somatic stem cells. World J Stem Cells 2013; 5(4): 163–171. CrossRef PubMed

Kapoor M., Martel-Pelletier J., Lajeunesse D. et al. Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol 2011; 7(1): 33–42. CrossRef PubMed

Kedzia A., Petriczko E., Tarka A. Placental growth hormone, pituitary growth hormone, insulin-like growth factor, and ghrelin in umbilical cord blood serum and amniotic fluid. Endokrynol Pol 2013; 64(4): 293–299. CrossRef PubMed

Kiely M., Cogan P., Kearney P. et al. Concentrations of tocopherols and carotenoids in maternal and cord bloodplasma. Eur J Clin Nutr 1999; 53(9): 711–715. CrossRef PubMed

Korzhevskiy D.E. Bases of histological technique. – St-Petersburg.: SpetsLit, 2010.

Lowin T., Weidler C., Jenei-Lanzl Z. et al. Relationship between placenta growth factor 1 and vascularization, dehydroepiandrosterone sulfate to dehydroepiandrosterone conversion, or aromatase expression in patients with rheumatoid arthritis and patients with osteoarthritis. Arthritis Rheum 2012; 64(6): 1799–1808. CrossRef PubMed

Neogi T., Zhang Y. Epidemiology of OA. Rheum Dis Clin North Am 2013; 39(1): 1–19. CrossRef PubMed

Olson S., Horne P., Furman B. et al. The role of cytokines in posttraumatic arthritis. J Am Acad Orthop Surg 2014; 22(1): 29–37. CrossRef PubMed

Orth P., Rey-Rico A., Venkatesan J. et al. Current perspectives in stem cell research for knee cartilage repair. Stem Cells Cloning 2014; 7: 1–17.

Perveen S., Altaf W., Vohra N. et al. Effect of gestational age on cord blood plasma copper, zinc, magnesium and albumin. Early Hum Dev 2002; 69(1–2): 15–23.

Regenerative biology and medicine. Cell technologies in therapy of nervous system diseases/ Ed. By V.N. Yarygin, V.P. Puzyrev, K.N. Yarugin, V.V. Semchenko. – Yekaterinburg – Moscow – Omsk – Tomsk – Khanty-Mansiysk: Omsk Regional Printing Office, 2015.

Roman-Blas J., Jimenez S. NF-kappaB as a potential therapeutic target in osteoarthritis and rheumatoid arthritis. Osteoarthritis Cartilage 2006; 14(9): 839–848. CrossRef PubMed

Scanzello C., Goldring S. The role of synovitis in osteoarthritis pathogenesis. Bone 2012; 51(2): 249–257. CrossRef PubMed

Sharifi B., Mosavi Zadeh A., Famil Tokhmechi M. Efficiency assessment of topical fetal human cord blood serum on alkaline corneal epithelial defects healing in a rabbit model. Life Science Journal 2013; 10(7): 731–734.

Sharma N., Goel M., Velpandian T. et al. Evaluation of umbilical cord serum therapy in acute ocular chemical burns. Invest Ophthalmol Vis Sci 2011; 25(2): 1087–1092. CrossRef PubMed

Sokolove J., Lepus C. Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Biomed Res Int 2013; 5(2): 77–94.

Sun R., Zhang B., Chen L., Sun J. Role of nuclear factor of activated T cells 1 in the pathogenesis of osteoarthritis. Exp Ther Med 2014; 7(1): 195–198.

Sun T., Ma Q. Repairing neural injuries using human umbilical cord blood. Mol Neurobiol 2013; 47(3): 938–945. CrossRef PubMed

Tutdibi E., Hunecke A., Lindner U. et al. Levels of cytokines in umbilical cord blood in relation to spontaneous term labor. J Perinat Med 2012; 40(5): 527–532. CrossRef PubMed

Vvedenskiy B.P., Dedukh N.V., Kovalev G .A., Sandomirskiy B.P. Influence of low temperatures and ethanol on the state of structural elements of the knee joint. Orthopaedics, Traumatology and Prosthetics 2013; (2): 28–33.

Vvedenskiy B.P., Kovalev G.A., Tynynyka L.N. et al. Cryopreserved cord blood serum in treatment of destructive and dystrophic processes in joints. Bulletin of Urgent and Recovery Medicine 2012; 13(1): 41–43.

Wang H.S., Kuo P.Y., Yang C.C. et al. Matrix metalloprotease-3 expression in the medial plica and pannus-like tissue in knees from patients with medial compartment osteoarthritis. Histopathology 2011; 58(4): 593–600. CrossRef PubMed




How to Cite

Vvedenskiy, B. P., Kovalev, G. A., Dedukh, N. V., & Sandomirskiy, B. P. (2016). Effect of Cryopreserved Cord Blood Serum Administration on Structural Changes of Joints During Induced Gonarthrosis in Rats. Problems of Cryobiology and Cryomedicine, 26(4), 361–374. https://doi.org/10.15407/cryo26.04.361



Cryomedicine, Clinical and Experimental Transplantology