Transformation of Erythrocytes During Cryopreservation with Oxyethylated Glycerol Derivatives with n=25 and n=30 Polymerization Degree
Keywords:oxyethylated glycerol, erythrocyte, shape, diameter, cryopreservation
This paper presents experimental data on the effect of oxyethylated glycerol derivatives with n=25 and n=30
polymerization degree (OEG<sub>n=25</sub> and OEG<sub>n=30</sub>) on shape and diameter of human erythrocytes at the stages of cryopreservation. The shape and diameter of the cells before and after cryopreservation were studied in ‘live drop’ by means of immersion microscopy. It has been shown that the contact with the investigated cryoprotective solutions and following low temperature preservation led to erythrocyte transformation. Character of change in their shape and diameter before and after cryopreservation depended upon the polymerization degree and concentration of cryoprotectant. It was established that 40% OEG<sub>n=25</sub> and OEG<sub>n=30</sub> solutions at the exposure stage caused cell deformation and agglutination. The largest number of erythrocytes of discoid shape was maintained after cryopreservation in 30% OEG<sub>n=25</sub> and OEG<sub>n=30</sub> solutions. It was found that after cooling-warming the cells in 30% OEG<sub>n=30</sub> solution their diameter increased, while using 30% concentration of OEG<sub>n=25</sub> this parameter was within the normal range.
Probl Cryobiol Cryomed 2016; 26(4): 349–360
Albertson P.A. Partition of cell particles and macromolecules. Stockholm: Almqvistand Wiksell; 1971.
Babijchyk L.A. Conformation changes in erythrocytes under the effect of PEO-1500 cryoprotection. Probl Cryobiol 1997; 1–2: 95–99.
Babijchyk L.A., Zemlyanskikh N.G. Optimization and advantages of washing-out method for erythrocytes cryopreservation with PEO-1500. Probl Cryobiol 2001; 1: 35–41.
Bessis M. Living blood cells and their ultrastructure. New York: Springer Verlag; 1973.
Chang A., Kim Y., Hoehn R., Jernigan P. Cryopreservation packed red blood cells in surgical patients: past, present and future. Blood Transfusion 2016. 8 (1): 1–7.
Esipova Y.S., Nikolenko A.V., Kompaniets A.M. Cryoprotective effect of oxyethylated glycerol with n = 30 polymerisation degree under human erythrocytes freezing. Probl Cryobiol 2008; 18 (1):114–118.
Gedde M.M. Yang Е., Huestis W.H. Membrane potential and human erythrocyte shape. Biophys J 1997; 72 (3): 1220–1233. CrossRef
Glaser R., Fujii T., Muller P. et al. Erythrocyte shape dynamics influence of electrolyte conditions and membrane potential. Biomed Biochim Acta 1987; 46 (2): 327–333.
Grachev A.Ye., Nakastoev I.M., Gemdzhan E.G. et al. Effect of duration of cryopreservation of erythrocytes on quality and efficiency of their transfusions. Gematologiya i Transfusiologiya 2013; 58 (2): 32–36.
Guchok V.M., Kozlova V.F. On Cumulative properties of 1,2-propanediol, glycerol and oxyethylated glycerol M.W. 1412 and their tolerance by rats. Kriobiologiya i Kriomeditsyna 1983; 11: 24–28.
Khary K., Foo J., Howard J. Shapes of red blood cells: comparison of 3D confocal images with the bilayer-coupe model. Cell Mol Bioeng 2010; 1 (2–3): 173–181.
Kompaniets A.M., Nikolenko A.V., Chekanova V.V., Yesipova Y.S. Cryoprotective efficiency of media based on oxyethyl derivatives of polyols during freezing of human erythrocytes. Probl Cryobiol 2008; 18 (3): 229–301.
Kozlova V.F. Morphological aspects of cell, tissue and organ response to oxyethylated glycerol (OEG) administered to animal's body. Kriobiologiya i Kriomeditsyna 1980; 6: 29–34.
Kuleshova L.G. Transformation of human erythrocytes in the solutions of non-electrolytes of H-Alcohol series. Part 1. Morphological aspect of interaction. Probl Cryobiol 2006; 16: 164–175.
Lybyany V.G., Bredikhina L.P., Shrago M.I. The cryoprotectant activity of GOE oligomers in low-temperature erythrocytes preservation. Kriobiologiya i Kriomeditsyna 1981; 8: 34–40.
Macczak A., Bukowska B., Michalowicz J. Comparative study of the effect of BPA and its selected analogues on hemoglobin oxidation, morphological alterations and hemolytic changes in human erythrocytes. Comp Biochem Physiol and Toxicol Pharmacol 2015; 176: 62–70. CrossRef PubMed
Miheeva L.M., Zaslavsky B.Y., Rogozhin S.V. Choice of an aqueous polymer two-phase system for cell partition. Biochim Biophys Acta. 1978; 542 (1): 101–106. CrossRef
Pavlova T.V., Pozdnyakova N.M., Proschayev K.I. Change in morphofunctional properties of erythrocytes and content of oxygen in them in patients with risk of premature ageing. Donozologiya: Proc. of Rep. the 7th International Scientific Conference; 2011 Dec 15-16; St-Petersburg, Russian; 2011: 446–448.
Pellerin-Mendes C., Million L., Marchand-Arvier M. et al. In vitro study of the protective effect of trehalose and dextran during freezing of human red blood cells in liquid nitrogen. Cryobiology 1997; 35 (2): 173–186. CrossRef PubMed
Sheet M.P., Singer S.J., Biological membranes as bilayer couples. A mechanism of dryg-erythrocyte interaction. Proc Natl Acad Sci 1974; 71: 4457–4461. CrossRef
Shrago M.I., Kalugin Yu.V., Kochurovskaya G.G. et al. Effect of oxyethylation on some physical-chemical and biological characteristics of glycerol. Kriobiologiya i Kriomeditsyna 1976; 2: 31–32.
Sputtek A., Langer R., Singbartl G. Cryopreservation of red blood cells with the non-penetrating cryoprotectant hydroxyethyl starсh. CryoLetters 1995; 16 (2): 283–288.
Terekhov N.T., Poluboyarinova A.G., Kushko O.V. Functional state of erythrocytes frozen with PVP and their clinical study. Problemy Gematologii i Perelivaniya Krovi 1977; 2: 16–19.
Quan G.B., Zhang L., Guo Y. et al. Intracellular sugars improve survival of human red blood cells cryopreservation at –80°C in the presence of polyvinylpyrrolidone and human serum albumin. CryoLetters 2008; 28 (2): 95–108. PubMed
Voejkov V.L. Physical-chemical and physiological aspects of erythrocytes sedimentation reaction. Uspekhi Phisiologisheskikh Nauk 1998; 29 (4): 55–73.
Weng X., Cloutier G., Pibarot P. et al. Comparison and simulation of different levels of erythrocyte aggregation with pig, horse, sheep, calf, and normal human blood. Biorheology 1996; 33 (4):365–377. CrossRef
Zubov P.M., Zemlyanskikh N.G., Babijchyk L.A. Modification of protein composition of erythrocyte membrane-cytoskeletal complex under PEO-1500 Effect. Probl Cryobiol 2006; 16 (pt. 2): 164–175.
Zubov P.M. Change in lipid asymmetry of erythrocyte membranes of cord and donor's blood when cryopreserving with PEO-1500. Vestnik Problem Biologii i Meditsyny 2013; 2(2): 109–112.
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