Cryopreserved Placental Explants Increase Lifespan of Male Mice and Change Survival Features of Female Mice

Authors

  • Vladimir Yu. Prokopyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleg V. Chub Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Nadezhda A. Shevchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oksana V. Falko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Irina B. Musatova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Victoria V. Lazurenko Kharkiv National Medical University, Kharkiv
  • Aleksandra N. Tischenko Kharkiv National Medical University, Kharkiv
  • Olga S. Prokopyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo27.02.143

Keywords:

lifespan, survival, mice, placenta, cryopreserved products

Abstract

According to the WHO data, to date the population lifespan is increasing worldwide due to infant mortality decrease, rather than the duration and life quality of elderly people. Most effective geroprotectors affect the immune and nervous systems, cell
cycle, metabolism of carbohydrates, stimulate autophagy. Bioobjects of placental origin possess similar properties. The research aim was to study the effect of cryopreserved human placental explants on lifespan of BALB/c mice. Kaplan-Meier survival curves were analyzed and survival indices were calculated. The differences were determined using the Log-rank test. It has been shown that implantation of cryopreserved placental explants to experimental animals significantly increases the indices in male mice as follows: median of lifespan - by 17.6%; average life expectancy – by 18.1%; 90% survival rate – by 20.8%; maximum lifespan – by 17.9%. The administration of placental explants to female mice did not affect their lifespan, but reduced the probability of death in the reproductive period.

Probl Cryobiol Cryomed 2017; 27(2): 143–150

Author Biographies

Vladimir Yu. Prokopyuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproduction System

Oleg V. Chub, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproduction System

Nadezhda A. Shevchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproduction System

Oksana V. Falko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproduction System

Irina B. Musatova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproduction System

Victoria V. Lazurenko, Kharkiv National Medical University, Kharkiv



Aleksandra N. Tischenko, Kharkiv National Medical University, Kharkiv



Olga S. Prokopyuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproduction System

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Published

2017-06-25

How to Cite

Prokopyuk, V. Y., Chub, O. V., Shevchenko, N. A., Falko, O. V., Musatova, I. B., Lazurenko, V. V., Tischenko, A. N., & Prokopyuk, O. S. (2017). Cryopreserved Placental Explants Increase Lifespan of Male Mice and Change Survival Features of Female Mice. Problems of Cryobiology and Cryomedicine, 27(2), 143–150. https://doi.org/10.15407/cryo27.02.143

Issue

Section

Cryomedicine, Clinical and Experimental Transplantology