Activity of Chymase, Tonin and Calpains in Rat Tissues Under Moderate Craniocerebral Hypothermia
The effect of moderate (32°C) craniocerebral hypothermia (CCH) on the activity of specific proteases such as chymase, tonin and calpains was studied. The proteases activity was determined in the blood serum, tissues of the central nervous system structures (cortex, hypothalamus, brain stem, cerebellum) and internal organs (heart, liver, lungs, kidneys) in animals. It has been revealed that the moderate CCH performed together with anesthesia promotes a sharp suppression of chymase and especially tonin activities (by 3-5 orders), accompanied with significant activation (in 10–50 times) of calpains. In comparison to the control the CCH led to more significant changes were found in hypothalamus, cerebellum and liver (for chymase), in the cerebellum and liver (for calpains), and if compared with the action of anesthesia the differences were present in the cerebellum, lungs, heart and kidneys (for tonin), in hypothalamus and cerebellum (for calpains).
Probl Cryobiol Cryomed 2017; 27(3): 230–241
Babijchuk G.О., Samokhina L.M., Shylo O.V. et al. Chymase, tonin and calpains under hypometabolic state in rats. Probl Сryobiol 2005; 15(3): 465–466.
Bobr О.А., Myadelets O.D., Dubovsky V.V. Dynamics of the population of mast cells during the wound process in rats subjected to hypobiotic conditions (starvation, hypothermia). Vestnik of Vitebsk State Medical University 2006; 5(4): 21–27.
Chakraborti S., Alam M.N., Paik D. et al. Implications of calpains in health and diseases. Indian J Biochem Biophys 2012; 49(5): 316–328.
Emirbekov E.Z., Pashaeva M.E., Aydunbekov F.T., Magomedov K.K. Influence of cerebramin and mild hypothermia on freeradical processes in rat's brain within carotid artery occlusion. Fundamental Research 2013; 10(part 4): 797–801.
Granic I., Nyakas C., Luiten P.G. et al. Calpain inhibition prevents amyloid-beta-induced neurodegeneration and associated behavioral dysfunction in rats. Neuropharmacol 2010; 59(4–5): 334–342.
Hochachka P.W. Defense strategies against hypoxia and hypothermia. Science 1986; 231(4735): 234–241. CrossRef
Inoue K., Nishimura H., Kubota J. et al. Alternative angiotensin II formation in rats arteries occurs only at very high concentrations of angiotensin I. Hypertension 1999; 34(3): 525–530. CrossRef PubMed
Karpenko М.N., Tikhomirova M.S. The role of calpaines in the regulation of synaptic transmission. Neuroscience and Behavioral Physiology 2014; 100(4): 385–393.
Korolev V.V., Babiychyk G.A., Begunov V.G. et al., inventors. Apparatus for cooling and warming of the brain. Certificate of Authorship of the USSR №904695. 1982 Feb. 15.
Lesnikov D.V., Radushkevich V.L. Craniocerebral hypothermia in intensive therapy complex of metalcohol psychosis. Prikladnye Informatsyonnye Aspekty Meditsyny 2014; 17(1): 111–114.
Ley O., Bayazitoglu Y. Effect of physiology on the temperature distribution of a layered head with external convection. Int J Heat and Mass Transfer 2003; 46(17): 3233–3241. CrossRef
Lomako V.V., Samokhina L.M. Effect of rhythmic cooling on some ethological and biochemical indices in rats with experimental depression. Probl Cryobiol 2011; 21(1): 22–33.
Lomako V.V., Shylo A.V., Babijchuk G.A., Samokhina L.M. Craniocerebral hypothermia stimulates reactions of limited proteolysis in rat tissues. Probl Cryobiol Cryomed 2016; 26(3): 238–248. CrossRef
Lomonosova J.N., Shenkman B.S., Nemirovskaya T.L. Calpinedependent processes in skeletal muscles with functional discharge. Doklady Akademii Nauk 2014; 458(6): 714–717.
Lysenko L.A., Kantserova N.P., Rendakov N.L., Nemova N.N. Calpaines and their endo- and exogenous regulators at various models of neurodegeneration. Rus J Bioorganic Chem 2014; 40(6): 695–702.
Medeiros R., Kitazawa M., Chabrier M.A. et al. Calpain inhibitor A-705253 mitigates Alzheimer's disease-like pathology and cognitive decline in aged 3xTgAD mice. Am J Pathol 2012; 181(2): 616–625. CrossRef PubMed
Nurmagomedova P.M., Abasova M.M., Emirbekov E.Z. The activity of Ca2+-dependent neutral proteases in squirrel tissues in the dynamics of hibernation and during self-warming after induced awakening. Bull Exp Biol Med; 2011: 151 (5): 509–512.
Samokhina L.M. Stress, hypertension and adaptation. Enzymes of vasoconstriction and destruction at stress, hypo- and hypertension. The rhythmic cold effects. Lambert Academic Publishing, Saarbrucken; 2015.
Samokhina L.M., Dubinin A.A., inventors. A method for determining the activity of proteinases or their inhibitors in biological fluids. Patent of Ukraine №20171, IPC C 12 Q 1/38. 1997 Dec 25.
Samokhina L.M., Babijchuk G.О., Lomako V.V. Influence of the rhythmic cold effect on the activity of calpaines in rats with alcohol-dependent hypertension. Theoretical and practical aspects of modern cryobiology. Proc Int Correspond Sci Pract Conf; 2014 March 24; Syktyvkar, Russian; 2014: 373–382.
Samokhina L.M., Lomako V.V., Shylo O.V. Chymase, tonin and calpains under conditions of natural hibernation in hamsters. Dosyagnennya Biologii ta Medytsyny 2010; (2): 29–32.
Samokhina L.M. Lomako V.V., Shylo A.V. Influence of cold exposure on the activity of chymase and tonin in the tissues of rats. Biofizika Zhyvoy Kletki; 2014; 10: 180–182.
Samokhina L.M., Starodub N.F., Babiychuk G.A., Lomako V.V. Rhythmic cold effect on activity of elastases in female rats with alcohol-depended hypertension. Probl Cryobiol 2012; 22(1): 49–60.
Shimizu M., Tanaka R., Uchida M. et al. Effect of Angiotensin II Type 1 receptor blocker on cardiac angiotensin-converting enzyme and chymase-like activities, and cardiac fibrosis in cardiomyopathic hamsters. J Vet Med Sci 2006; 68(3): 227–233. CrossRef PubMed
Shiraha H., Glading A., Chou J. et al. Activation of m-calpain (calpain II) by epidermal growth factor is limited by protein kinase A phosphorylation of m-calpain. Mol Cell Biol 2002; 22(8): 2716–2727. CrossRef PubMed
Suzuki K., Hata S., Kawabata Y., Sorimachi H. Structure, activation, and biology of calpain. Diabetes 2004; 53(1): S12–S18.
SzczygieіJ., Mazurek J., Swiatkowski A. et al. The neuroprotective effect of mild therapeutic hypothermia after out-of-hospital cardiac arrest with successful reanimation – a case report. Pol Merkur Lekarski 2016: 40(237): 177–181.
Takai S., Shiota N., Sakaguchi M. et al. Characterization of chymase from human vascular tissues. Clin Chim Acta 1997; 265(1): 13–20. CrossRef
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.
23, Pereyaslavskaya str., Kharkov, Ukraine
Tel. +38057 373 4143; Fax +38057 373 5952