Effect of Postural Changes, Postprandial Load, N. Vagus Pharmacological Denervation and Cryodenervation on Heart Rate Variability in Rats

Nikolai A. Chizh


Heart rate variability (HRV) spectral analysis was applied to establish how postural change, accompanied by a change in the central venous pressure are associated with a very low-frequency (VLF)-component of the spectrum. Under postprandial load the total power of HRV spectrum was found out to decrease by 1.9 times due to reducing the very low-frequency component of the spectrum. A statistically significant decrease in the LF/HF ratio from 6.7 down to 4.8 due to an increase in the high-frequency range was noted. After treating the animals with Loperamide and Atropine sulfate, which inhibited a motor-evacuation function of gastrointestinal tract, there was established the effect of digestive system on HRV within the LF and HF ranges. The manifestation rate of HRV changes as a result of nervus vagus cryodenervation was shown to be significantly lower than after pharmacological denervation with Atropine sulfate. The ratio of LF/HF components decreased from 3.4 down to 1.3 due to the augmentation of high-frequency component of the spectrum. The findings demonstrated the parasympathetic nervous system to control all the ranges of spectrum and play a major role in HRV formation.

Probl Cryobiol Cryomed 2017; 27(3): 266-278


heart rate variability; spectral analysis; central venous pressure; nervus vagus; cryodenervation

Full Text:



Ambarish V., Barde P., Vyas A., Deepak K. Comparison between pre-prandial and post-prandial heart rate variability (HRV). Indian J Physiol Pharmacol 2005; 49 (4): 436–442. PubMed

Bayevsky R.M., Chernikova A.G. Heart rate variability analysis: physiological foundations and main methods. Cardiometry 2017; 6 (10): 66–76. CrossRef

Breus T.K., Chibisov S.M., Bayevskii R.M. et al. Chronostructure of heart biorhythms and external environmental factors. Moscow; 2002.

Chizh N.A. Parameters of spectral analysis of heart rate variability in rats. Probl Cryobiol Cryomed 2015; 25 (3): 235–245. CrossRef

Chizh N.A. Ways of registering electrocardiograms in rats for analyzing heart rate variability. Exp Clin Med 2015; 68 (3): 44–47.

Chizh N.A. Effect of respiration and vascular tone on heart rate variability in rats. Probl Cryobiol Cryomed 2016; 26 (3): 260–270. CrossRef

Chizh N.A., Marchenko L.N., Belochkina I.V. et al. Ultrastructure of hepatic artery after cryodenervation. Ukrainskyi Morfologichnyi Almanakh 2008; 6 (3): 84–87.

Heart rate variability: standards of measurement, physiological interpretation, and clinical use. Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology. Circulation 1996; 93 (5): 1043–1065. CrossRef PubMed

Houle M.S., Billman G.E. Low-frequency component of the heart rate variability spectrum: a poor marker of sympathetic activity. Am J Physiol 1999; 276: 215–223.

Khayutin V.M., Lukoshkova E.V. Heart rate oscillation: spectral analysis. Journal of Arrhythmology 2002; (26): 10–18.

Kotelnikov S.A., Nozdrachev A.D., Odinak M.M. et al. Ideas about mechanisms of heart rate variability. Human Physiology 2002; 28(1): 130–143.

Lutsenko D.G., Shylo A.V., Marchenko L.N. et al. Peculiarities of heart rhythm regulation at different types of cold acclimation in rats. Probl Cryobiol Cryomed 2013; 23 (2): 105–115.

Mikhailov V.M. Heart rate variability. Experience of practical application of the method. Ivanovo; 2000.

Mohrman D.E., Heller L.J. Cardiovascular Physiology. Chapter 8.

Central Venous Pressure: An Indicator of Circulatory Hemodynamics 2003: P. 146–156.

Triedman J.K., Saul J.P. Blood pressure modulation by central venous pressure and respiration. Buffering effects of the heart rate reflexes. Circulation 1994; 89 (1): 169–179. CrossRef

Yabluchansky N.I., Martynenko A.V. Heart rate variability. Guidelines for practitioner. Kharkov; 2010.

Zaitseva O.S., Spivak E.M. Postprandial hemodynamic changes in abdominal vessels of healthy children. Perm Medical Journal 2017; 34 (1): 32–34.

DOI: https://doi.org/10.15407/cryo27.03.266


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Institute for Problems of Cryobiology and Cryomedicine

23, Pereyaslavskaya str., Kharkov, Ukraine

Tel. +38057 373 4143; Fax +38057 373 5952

e-mail: journal@cryo.org.ua