Cardiomyocyte Ultrastructure of Rats with Experimental Myocardial Infarction After Therapeutic Hypothermia and Mesenchymal Stromal Cell Administration
Keywords:experimental myocardial infarction, therapeutic hypothermia, mesenchymal stromal cells, ultrastructure, mitochondria
We studied the ultrastructural changes in cardiomyocytes during necrosis development and re-modelling of the rat heart following experimental myocardial infarction (MI) and performing therapeutic hypothermia and administration of allogeneic mesenchymal stromal cells (MSCs). The infarction was provoked via ligation of left coronary artery. Therapeutic hypothermia was performed in cold chamber for 60 min achieving 4Â°C skin temperature in the collar zone. The suspension of allogeneic cryopreserved MSCs of placenta with 1.2x10Â° cells/ml concentration was intravenously administered. In the animals with MI treated with MSCs and a combination of MSCs and hypothermia we revealed the normalization of mitochondrial structure, appearance of small dense mitochondria, the presence of a large number of glycogen granules, testifying thereby to a sufficient oxygen supply into cardiomyocytes and normalization of synthetic processes together with improved microcirculation under MSCs factors. The combination of therapeutic hypothermia with MSCs administration at the background of MI largely promoted the activation of compensatory-regenerative processes in cardiomyocytes.
Probl Cryobiol Cryomed 2017; 27(4): 334-347
Abdel-Latif A., Bolli R., Tleyjeh I.M. et al. Adult bone marrowderived cells for cardiac repair: A systematic review and metaanalysis. Arch Intern Med 2007; 167(10): 989â€“997.
Avtandilov G.G. Medical morphometry. Moscow: Meditsina; 1990.
BabayevÐ° G.G, Chyzh M.O., Galchenko S.E., Sandomyrsky B.P, inventors. Method for myocardial infarction simulation. Patent of Ukraine IPC G 09B 23/28. 2011 Dec 12.
Cherpachenko N.M., Sokolova R.I. Histochemical and electronmicroscopic characteristics of mitochondria in myofibrils located outside of the area of experimental myocardial infarction. Arkhiv Patologii 1970; 35(2): 23â€“30.
Chizh M.O. Ways of registering electrocardiograms in rats for analyzing heart rate variability. Exp Clin Med 2015; 68(3): 44â€“47.
Egorova M.V., Krakhmal N.V., Afanasiev S.A. et al. Comparative analysis of changes in myocardial structure at the separate and combined post-infarction and diabetic heart damages. Fundamentalnye Issledovaniya 2013; 11(7): 77â€“82.
Hecht A. Introduction to experimental basics of current pathology of heart muscle: Moscow: Meditsina; 1975.
Holzer M., Bernard S.A., Hachimi-Idrissi S. et al. Collaborative group on induced hypothermia for neuroprotection after cardiac arrest. Hypothermia for neuroprotection after cardiac arrest: systematic review and individual patient data meta-analysis. Crit Care Med 2005; 33(2): 414â€“418. CrossRef PubMed
Konoplyannikov M.A., Kalsin V.A., Averyanov A.V. Stem cells for therapy of ischemic heart disease: achievements and prospects. Klinicheskaya Praktika 2012; 3(3): 63â€“77.
Kovalenko V.M., Dorogoy A.P., Sirenko Yu.M. Diseases of circulatory system in mortality structure of population of Ukraine: myths and reality. Ukrainian Cardiology Journal 2013; 4: 22â€“29.
Kruglyakov P.V., Sokolova I.V., Polyntsev D.G. Ð¡ell therapy for myocardial infarction. Tsitologiya 2008; 50(6): 521â€“527.
Maureira P., Marie P.Y., Yu F. et al. Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling. J Biomed Sci 2012; 19(1): 93â€“104. CrossRef PubMed
Rech T.H., Vieira S.R. Mild therapeutic hypothermia after cardiac arrest: mechanisms of action and protocol development. Rev Bras Ter Intensiva 2010; 22(2): 196â€“205.
Samuilov V.D. The biochemistry of programmed cell death (apoptosis) in animals. Soros Educational Journal 2010; 10(7): 18â€“25.
Solodovnikova I.M., Saprunova V.B., Bakeyeva L.E., Yaguzhinsky L.S. Dynamics of mitochondrial ultrastructure in small pieces of cardiac tissue under long-term anoxic conditions. Tsitologiya 2006; 48(10): 848â€“855.
Stepanov A.V., Baidyuk E.V., Sakuta G.A. Characteristics of rat cardiomyocytes mitochondria in chronic heart failure. Tsitologiya 2016; 58(11): 875â€“882.
Svitina H., Kalmukova O., Shelest D. et al. Cellular immune response in rats with 1,2-dimethylhydrazine-induced colon cancer after transplantation of placenta-derived stem multipotent cells. Cell and Organ Transplantology 2016; 4(1):55â€“60. CrossRef
Trofimova Ð.V., Chizh N.Ð., Belochkina I.V. et al. Morphological characteristics of heart after induction of therapeutic hypothermia and the introduction of mesenchymal stromal cells in therapy of experimental myocardial infarction. Morphologia 2016; 10(3): 288â€“292.
Tsyplenkova V.G., Sutyagin P.V., Suslov V.B., Oettinger A.P. Cardiomyocyte mitochondria characteristics in different heart diseases and in experiments. Mezhdunarodny Zhurnal Prikladnykh i Fundamentalnykh Issledovanij 2014; 8 (Pt 2): 53â€“56.
Weakley B.S. Beginners' handbook in biological electron microscopy. Churchill Livingstone; 1972.
Zadnipryany I.V., Tretiakova O.S., Sataeva T.Ð . Morphological substratum of secondary mitochondrial dysfunctions at transitional ischemia of the myocardium at infant rats. Tavricheskiy Mediko-Biologicheskiy Vestnik 2013; 16(3): 174â€“178.
Zamostyanova G.B., Urakov A.L. Pharmacological possibility of limiting calcium energy-dependent accumulation by mitochondria of the ischemic myocardium. Farmakologiya i Toksikologiya 1989: 52(4): 27â€“29.
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