Temperature Changes During Cryoeffect Potentiated With Distilled Water Assessed in Porcine Liver Model Without Splanchnic Blood Flow

Oleksiy I. Dronov, Dmytro I. Khomenko, Petro P. Bakunets, Viktoria V. Teteryna


The efficiency of local cryoeffect on pathological tissue area depends on the values of achieved temperature at a certain depth. This study presents the results on comparative analysis of the dynamics of temperature changes measured by intraoperative four-channel thermocouple unit KVIT-4 at discrete depths of 3, 8, 13 and 18 mm. We carried out two successive cycles of local cryoeffect with preliminary introduction of a distilled water into parenchyma of porcine liver (main group) and without any additional action (control group) in experimental model of porcine liver without splanchnic blood flow. The average temperature in animal liver tissue of the main group at a distance of 3 mm deep from the cryoapplicator operating surface was lower by 35 degrees at the end of the 10th min of cryoeffect of the 2nd cycle if compared with the control (–85.2 and –49.2°C, respectively), at a depth of 8 mm it was lower by 52,5 degrees (–61.1 and –8.5°C, respectively), at 13 mm this index was lower by 51,5 degrees (–59.5 and –3.0°C, respectively), and at 18 mm it was lower by 40,4 degrees (–40.5 and –0.1°C, respectively). An introduction of a distilled water into biological tissue 5 min prior to the initiation of local cryoeffect increased its effectiveness, as evidenced by the reaching of lower temperatures in the areas distant from the operating surface of the cryoapplicator.

Probl Cryobiol Cryomed 2017; 27(4): 348–355


odestruction; local cryoeffect; enhanced cryodestruction; distilled water; thermocouple

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Cooper I.S., Lee A. Cryostatic congelation: a system for producing a limited controlled region of cooling or freezing of biologic tissues. J Nerv Ment Dis 1961; 133(3): 259–263. CrossRef

Degtev M.V., Kozhevnikov E.V., Kozhevnikov V.A. The method of warts and hemangiomas cryodestruction using sodium etamzylate. Vestnik Dermatologiii Venerologii 2011; (1): 69–72.

Chzhao A.V., Chekmareva I.A., Paklin O.V. et al. Early morphological changes of hepatocytes after cryodestruction. Journal of Anatomy and Histopathology 2017; 6(1): 109–114. CrossRef

Gage A.A., Baust J. Mechanism of tissue injury in cryosurgery. Cryobiology 1998; 37(3): 171–186. CrossRef PubMed

Goltsev A.N., Diabina O.A., Ostankov M.V. et al. Cancer stem cells in tumor pathogenesis after cryoablation. Probl Сryobiol Сryomed 2015; 25(3): 205–218. CrossRef

Khomenko D.I., Lukyanova N.Yu., Kozachuk E.S. Study results of local cryosurgery in experimental models of 36% NaCl, distilled H2O and 0,9% NaCl and prospects of their use during cryofixation of pancreas solid tumors. Advancements in Clinical and Experimental Medicine: Proc. of Scientific Conference Devoted to the 60th Anniversary of I. Horbachevsky Ternopil State Medical University; 2017 June 14; Ternopil: TSMU; 2017: 182–184.

Khomenko D.I., Lukyanova N.Yu., Onoshko M.M., Kozachuk E.S. Study results of morphological changes of Guerin carcinoma cells depending on the exposure time in distilled H2O solution. Proc. of Scientific Conference of Young Scientists devoted to the 40th Anniversary of Shupyk National Medical Academy of Postgraduate Education; 2017 May 18; Kyiv; 2017: 91–93.

Kubyshkin V.A., Ionkin D.A., Kungurtsev S.V., Chzhao A.V. History of cryosurgery. Khirurgiya. Zhurnal im N.I. Pirogova 2015; (5): 62–74.

Shafranov V.V., Borkhunova E.N., Kostylev M.A. et al. The mechanism of destruction of biological tissue at a local cryodestruction. Vestnyk Rossiyskoy Akademii Estestvennykh Nauk 2012; (1): 68–77.

Shafranov V.V., Borhunova E.N., Taganov A.V. et al. Modern treatment technologies of large angiomas with critical localization in children. Al'manah Kliniceskoj Mediciny 2008; 17(2): 287–288.

Shafranov V.V., Ten Yu.V., Reznitsky V.I. et al. UHF and cryoeffect on biological tissues in experiment and clinics. Cryobiology 1988; (3): 38–42.

State Pharmacopoeia of Ukraine / 2nd ed. – Kharkiv: State Enterprise 'Ukrainian Scientific Pharmacopoeial Center for Quality of Medicinal Products'; 2014; 2: 724 p.

Tabolinovskaya T.D. Pustynsky I.N. Analysis of the efficiency of cryogenic treatment for tongue cancer. Head and Neck Tumors 2014; (1): 15–23.

Yu Q., Yi G., Zhao G., Zhang Y. Effect of vascular network and nanoparticles on heat transfer and intracellular ice formation in tumor tissues during cryosurgery. CryoLetters 2014; 35(2): 95–100.

DOI: https://doi.org/10.15407/cryo27.04.348


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