Structure of Myocardium of Young Rats with Simulated Alimentary Obesity Following the Application of Combined Rhythmic Extreme Cold Effects and Cryopreserved Cord Blood Nucleated Cells
Keywords:alimentary obesity, rats, rhythmic extreme cold exposures, cord blood nucleated cells, cardiomyocytes, cardiosclerosis, lipidosis
To date, a large amount of data has been accumulated, indicating a positive effect of rhythmic extreme cooling (â€“1200C) and medical products obtained from cord blood, both on different organs, systems, and on the whole body. The histological features of the tissues and blood vessels of the heart were studied in the experimental model of alimentary obesity. The appearance of collagen and elastic fibers of the myocardium of young rats before and after the combined application of rhythmic extreme cold exposures (RECEs) and cryopreserved preparation of cord blood nucleated cells (CBNCs) was histochemically assessed. It has been found that the structural organization of the myocardium of young rats with obesity was characterized by pronounced pathological changes associated with impaired blood circulation. Plethora and thrombosis of blood vessels, diapedemic hemorrhages, the myocardium hypertrophy, interstitial and perivascular edema, focal cardiosclerosis were revealed. To days 7 and 30 after the combined use of 9 sessions of the RECEs and CBNCs, the normalization of the heart morphological structure was noted. In the interstitial connective tissue we have observed a proliferation of fibroblasts, diffuse spread of histiocytes, which indicated the development of processes of neoangiogenesis, less expressed cardiosclerosis, perivascular and interstitial edema.
Probl Cryobiol Cryomed 2018; 28(2): 108â€“119
Agajanyan N.A., Bykov A.T., Medalieva R. Kh. Condition of nonspecific adaptable reactions of the organism and health levels at various modes of extreme cryogenic trainings. Human Ecology 2012; 10: 28â€“33.
Anisimova E.V., Kozlova I.V., Volkov S.V. et al. Pathology of digestive organs at adiposity (review). Saratov Journal of Medical Scientific Research 2011; 7(4): 851â€“856.
Babijchuk L.V., Koval S.N., Babijchuk G.A. Structure of myocardium of young hypertensive rats after injection of cryopreserved human cord blood nucleated cells. Probl Cryobiol and Cryomed 2016; 26(3): 271â€“287. CrossRef
Babiychuk G.O., Kozlov O.V., Lomakin I.I. et al. Cryochamber for experimental cooling of laboratory animals. Patent of Ukraine N 40168. 2009 March 25.
Babiychuk L.A., Grishchenko V.I., GurinÐ° T.M. et al. The method of cryopreservation of cord blood nucleated cells, including hematopoietic stem cells. Patent of Ukraine N 92227. Oct. 11.
Babiychuk L.A., Grishchenko V.I., Ryazantsev V.V. et al. The method of allocation of cord blood nucleated cells. Patent of Ukraine N 234999. 2007 May 25.
Babiychuk V.G., Marchenko V.S., Babiychuk G.A. Cold stress as corrector of functional architecture of blood brain barrier in aged rats. Probl Cryobiol and Cryomed 2012; 22(2): 107â€“117. Full Text
Baranov V.G., Baranov N.F., Belovintseva M.F. Sensitivity to insulin, glucose tolerance and insulin activity of blood in rats with alimentary obesity. Problems of Endocrinology 1972; 6: 52â€“58.
Belenkov Yu.N., Privalov E.V., Chekneva I.S. Cell therapy of chronic heart failure: types of stem cells used the results of recent clinical studies. Ð¡ardiology and cardiovascular disease 2008; 5: 4â€“18.
Bykov A. T., Rybkina V. A., Kovalenko V. V. Hemodynamics state at different modes of systemic air cryogenic exposure. European Researcher 2012; 34(11): 1929â€“1934.
Chernyavskaya E.A., Babiychuk V.G., Mamontov V.V. et al. Method of correction of vegetative disorders of the body of experimental animals with alimentary obesity. Patent of Ukraine N 108528. 2016 July 25.
Chumakova G.A., Veselovskaya N.G., Kozarenko A.A. et al. Heart morphology, structure, and function in obesity. Russian Journal of Cardiology 2012; 4(96): 93â€“99.
Gluckman E.D. Cord blood as the source of hemopoietic stemcells for transplantation. Cellular Transplantation and Tissue Engineering 2011; 6(1): 12â€“14.
Kochegura T.N., Efimenko A.Y., Akopyan Zh.A. et al. Stem cell therapy of heart failure: clinical trials, problems and perspectives. Cellular Transplantation and Tissue Engineering 2010; 5(2): 11â€“18.
Kondakov I.K., Koval S.N., Snegurskaya I.A. et al. To the problem of metabolic syndrome pathogenesis. Adipose tissue and markers of acute inflammation phase. Arterial Hypertension 2009; 3(5): 39â€“43.
Mendelevich D.M., Grishkina M.N. Mental disorders in alimentary obesity. Kazan Med J 2004; 85(5): 363â€“366.
Oleynik O.A., Samoylova Y.G., Vorozhtsova I.N. Clinical metabolic and molecular genetic mechanisms of formation of cardiovascular complication in obesity. Sib Med J 2011; 26(4): 16â€“21.
Petrenko A.Yu., Grischenko V.I. Transplantation of stem cells âˆ’ a promising direction of the xxi century therapy. Stem hemopoietic cells from different sources. IMJ 2003; 1: 123â€“129.
Sarkisov D.S., Petrov D.S. Mykroskopycheskaya technics M.: Medicine; 1996; 544 Ñ€.
Strukov A.I., Serov V.V. Patolohycheskaya anatomy. M.: Medicine; 1993; 688 p.
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