Kinetics of Phase Transformations During Cooling-Warming of Saccharomyces cerevisiae Cells in Alginate-Containing Cryoprotective Media

Victoria L. Ponomareva, Larisa G. Kuleshova, Igor P. Vysekantsev, Olena S. Onasenko, Olga О. Mykhailova


The effect of sodium alginate on the viability and kinetics of phase transformations of Saccharomyces cerevisiae cells during cryopreservation was investigated. There were determined the optimal conditions for yeast cell cryopreservation such as: the cooling rate of 1 deg/min in alginate-containing cryoprotective media (cell viability of (90.79 ± 2.01)% and (88.11 ± 1.75)% in solution of 1% sodium alginate and in combination of 5% dimethyl sulfoxide (DMSO) and 1% sodium alginate, respect tively). Using cryomicroscopy, the presence of sodium alginate in cryoprotective medium was shown to change the crystallization performance and to shift the phase transition zone towards lower temperatures. The beginning of phase transformations in the specimens, cooled in 5% DMSO solution was recorded at –8.5°C, in those, containing 1% sodium alginate and in a three-component system, comprising the combination of 1% sodium alginate and 5% DMSO, it was found at –16 and –15°С, respectively.


Probl Cryobiol Cryomed 2018; 28(3): 212223


cryopreservation; yeast; sodium alginate; viability; cryomicroscopy

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Aksenova PV, Aibazov AMM, inventors; State Scientific Institution Stavropol Scientific Research Institute of Livestock and Feed Production of the Russian Academy of Agricultural Sciences (RU), assignee. [Preservation medium for goat sperm]. Patent RU 2010118373A, 2010 Jun 05. Russian.

Balezin SA, Erofeyev BV, Podobayev NI. [Fundamentals of physical and colloid chemistry]. Moscow: Prosvescheniye; 1975. 398 p. Russian.

Belous АМ, Gordienko ЕА, Rozanov LF. [Freezing and cryoprotection]. Moscow: Vysshaya Shkola; 1987. 398 p. Russian.

Belous АМ, Shrago МI, Pushkar NS. [Cryopreservatives]. Kyiv: Naukova Dumka; 1979. 198 p. Russian.

Bolshakova LS, Litvinova EB, Zhmurina ND, Burtseva EI. [The influence of various technological factors on rheological characteristics of alginate gels]. Sovremennye Problemy Nauki i Obrazovaniya [Internet]. 2012; (6): [Cited Jul 9 2018] Available from: Russian.

Breunig HG, König K. Two-photon imaging of intact living plants during freezing with a flexible multiphoton tomography. Laser Physics Letters [Internet]. 2015; [Cited 2018 Aug 21]; 12(2): 025601. Available from:

Egorova NS. [Practical course on microbiology]. Moscow: Moscow State University; 1976. 307 p. Russian.

Fuller B, Green C, Grischenko VI. Cryopreservation for cell banking: current concepts at the turn of the 21st century. Problems of Cryobiology. 2003; (2): 62–83. Full Text

Gurina TM, Vysekantsev IP, Polyakova AL. Influence of speed of cooling in area of eutectic um temperature of water solution of cryoprotectant on viability of cages of Saccharomyces cerevisiae. Probl Cryobiol Cryomed. 2013; 24(1): 15–26. Full Text

Han B, Bischof JC. Direct cell injury associated with eutectic crystallization during freezing. Cryobiology. 2004; 48(1): 8–21. CrossRef PubMed

Kailasapathy K. Microencapsulation of probiotic bacteria: technology and potential applications. Curr Issues Intest Microbiol. 2002; 3: 39–48. PubMed

König K., Uchugonova A., Breunig H. G. High-resolution multiphoton cryomicroscopy. Methods. 2014; 66(2): 230–6. CrossRef PubMed

Kozlova OG. [Morphogenic analysis of crystals]. Moscow: Moscow State University; 1991. 224 p. Russian.

Kuleshova LG. Cryomicroscopic complex for cryobiological studies. Tsitologiya. 2004; 46(9): 809–10.

Mazur P, Cole KW. Roles of unfrozen fraction salt concentration and changes in cell volume in the survival of frozen human erythrocytes. Cryobiology. 1989; 26(1): 1–29. CrossRef

Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol Cell Physiol. 1984; 247(3): 125–42. CrossRef PubMed

Ponomareva VL, Vysekantsev IP, Gurina TM, Onasenko ES. [Study of the influence of cooling regimes and protective media containing sodium alginate on the viability of yeast cells Saccharomyces cerevisiae]. Bulletin of Problems Biology and Medicine. 2011; (1): 35–7. Russian.

Polezhaeva ТV, Zaitseva ОО, Khudyakov АN, et al. Use of pectin polysaccharides for cryopreservation of biological objects. Arch Biol Sci. 2014; 66(3): 1025–33. CrossRef

Shchipunov YA., Koneva EL, Postnova IV. [Homogeneous alginate gels: phase behavior and rheological properties]. Polymer Sciences Series A. 2002; 44(6): 1201–11. Russian.

Yusova AA, Gusev IV, Lipatova IM. [Properties of the hydrogels based on a mixture of sodium alginate with other polysaccharides of natural origin]. Khimiia Rastitel'nogo Syr'ia. 2014; (4): 59–66.



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