Effect of Different Cooling Regimens (Craniocerebral and Immersion Hypothermia, Surface Rhythmic Cold Exposures and Whole Body Cryostimulation) on Leukocyte Indices of Rat Blood

Victoria V. Lomako


A comparative analysis of leukocyte types number in rat blood smears at the craniocerebral (CCH 32°C) and  immersion  (IH  27°C)  hypothermia,  surface rhythmic  cold  exposures  (RCEs  5°C)  and  whole  body  cryostimulation (WBC –120°C), as well as in 24 hrs revealed the leukocytosis (at IH and 24 hrs after CCH and IH), leukopenia (at  CCH, RCEs and 24 hrs after WBC), lymphopenia, an increase in band and segmented cells (under all conditions except IH) and eosinophils (only at CCH and 24 hrs after WBC). Plasmatic cells (except WBC), polychromatophilic and immature cells (immediately and in 24 hrs after CCH and IH), cytoplasm plasmatization in some leukocytes (24 hrs after CCH and IH) were observed. Leukocyte indices showed the immunoreactivity disorder, decreased allergization, activation of cell immunity, nonspecific cell protection and inflammation (under all conditions, except IH), as well as microphages (at WBC and 24 hrs after CCH and IH), an effector component of immunity (at CCH, RCEs and 24 hrs after WBC), immediatetype hypersensitivity responses (except at CCH and 24 hrs after WBC). The nuclear shift index was increased (except RCEs and WBC). The adaptation index was raised only at IH.


Probl Cryobiol Cryomed 2018; 28(4): 293-310


craniocerebral hypothermia; immersion hypothermia; rhythmic cold exposures; whole body cryostimulation; leukocytes; integral leukocyte indices; rats

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Abdulkadyrov KM, editor. [Hematology, the newest guidance]. Moscow: EKSMO; 2004: 928 p. Russian.

Abel EL. Physiological correlates of the forced swim test in rats. Physiol Behav. 1993; 54(2): 309–17.


Alva N, Azuara D, Palomeque J, Carbonell T. Deep hypothermia protects against acute hypoxia in vivo in rats: a mechanism related to the attenuation of oxidative stress. Exp Physiol. 2013; 98(6): 1115–24.



Babiichuk VG, Kozlov AV, Lomakin II, Babiichuk GA, inventors; Institute for problems of cryobiology and cryomedicine of NAS of Ukraine, assignee. [Cryochamber for experimental cooling of laboratory animals]. Ukraine patent № 40168. 2009 March 25. Ukrainian.

Billeter AT, Galbraith N, Walker S, et al. TRPA1 mediates the effects of hypothermia on the monocyte inflammatory response. Surgery. 2015; 158(3): 646–54.



Brinkevich VN, Myadelets OD. [Quantitative changes in the peripheral blood during prolonged hypothermia]. Problems of Cryobiology. 1991; (3): 25–8. Russian. Full Text

Cuttell S, Hammond L, Langdon D, Costello J. Individualising the exposure of –110°C whole body cryotherapy: The effects of sex and body composition. J Therm Biol. 2017 Apr; 65: 41–7. CrossRef PubMed

Dolgushin II, Bukharin OV. [Neutrophils and homeostasis]. Ekaterinburg: UrО RAS Publishing, 2001: 284 p. Russian

Garkavi LKh, Kvakina EB, Ukolova MA. [Adaptive reactions and resistance of the organism]. Rostov-on-Don: Rostov University Publishing; 1990. 223 p. Russian.

Goranchuk VV, Shustov EB, Andreeva LI, et al. [Adaptation of a person to hyperthermia and changes in peripheral blood leukocytes]. Ros Fiziol Zhurn im. IM Sechenova. 2001; (9): 1208–16. Russian.

Kepinska M, Gdula-Argasinska J, Dabrowski Z, et al. Fatty acids composition in erythrocyte membranes of athletes after one and after a series of whole body cryostimulation sessions. Cryobiology. 2017; 74(1): 121–5.



Korolev VV, Babiychyk GA, Begynov VG, et al. inventors; Institute for problems of cryobiology and cryomedicine of NAS of Ukraine, assignee. [Apparatus for cooling and warming of the brain]. USSR C.A. (certificate of authorship) № 904695. 1982 Feb. 15.

Kulis A, Misiorek A, Marchewka J, et al. Effect of whole-body cryotherapy on the rheological parameters of blood in older women with spondyloarthrosis. Clin Hemorheol Microcirc. 2017; 66(3): 187–95.



Lomako VV, Babijchuk GA, Marchenko VS, et al. Noradrenergic processes in rat hypothalamus after repeated craniocerebral hypothermic treatment. Problems of Cryobiology. 1994; (4):11–7. Full Text

Lomako VV, Pyrozhenko LN. [Influence of hyperthermia on leukocyte indices]. Medychnyy Forum. 2015; (5 Pt 2): 50–2. Russian.

Lomako VV, Pyrozhenko LN. [Leukocytes at hypometabolic states]. Visnuk Problem Biologii i Medycyny. 2010; (3): 61–5. Russian.

Lomako VV, Shilo AV, Kovalenko IF, Babiichuk GA. Erythrocytes of hetero- and homoiothermic animals under natural and artificial hypothermia. J Evol Biochem Physiol 2015; 51(1): 58–66.


Lombardi G, Lanteri P, Porcelli S, et al. Hematological profile and martial status in rugby players during whole body cryostimulation. PLoS ONE [Internet]. 2013 Feb 1 [cited Dec 15 2017]; 8(2): e55803. Evaluable from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0055803 CrossRef PubMed

Lombardi G, Ziemann E, Banfi G. Whole-Body Cryotherapy in Athletes: From Therapy to Stimulation. An Updated Review of the Literature. Front Physiol [Internet]. 2017 May 2 [cited Dec 15 2017];8:258. Evaluable from: https://www.frontiersin.org/articles/10.3389/fphys.2017.00258/full CrossRef PubMed

Losyenok SA, Brovkina IL, Prokopenko LG. [Correction with ethanol and fat-soluble vitamins immunometabolic disorders in acute cold stress]. Chelovek i Zdoroviye. 2008; (3): 11–21. Russian.

Lugovskaya SA, Kozinets GI. [Hierarchy hematopoetic cells: kinetics, structure and function]. Klin Lab Diagnostic. 2009; (5):21–37. Russian.

Mustafina ZhG, Kramarenko YuS, Kobtseva VYu. [Integral hematological indices in the evaluation of the immunological reactivity of the organism in patients with ophthalmopathology]. Klin Lab Diagnostic. 1999; (5): 46–8. Russian.

Myadelets OD, Sukhanov AF. [The effect of total deep hypothermia on the morphological and functional state of skin neutrophilic leukocytes and macrophages]. Problems of Cryobiology. 1991; (2): 23–7. Russian. Full Text

Nadezhdin SV, Fedorova MZ, Pavlov NA, Zubareva EV. [Changes in the functional activity of leukocytes in conditions of acute overheating of the organism]. Nauchnyie Vedomosti. Biologiya. 2008; 43(3): 5–11. Russian.

Oleynik GA. [Leukocyte indices in prognosis of the course and outcome of cold injury]. International medical journal. 2010;16(2): 63–9. Russian.

Pervushin YuV. [Resonance mechanisms of the change of biological states]. Biofizika. 1991; 3(3): 534–6. Russian.

Pournot H, Bieuzen F, Louis J, et al. Time-course of changes in inflammatory response after whole-body cryotherapy multiexposures following severe exercise. PLoS ONE [Internet]. 2011 Jul 28 [cited Dec 15 2017]; 6(7): e22748. Evaluable from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0022748 CrossRef

Raznatovskaya EN. Integral indices of endogenous intoxication in patients with chemoresistant pulmonary tuberculosis. Aktualʹni pytannya farmatsevtychnoyi i medychnoyi nauky ta praktyky. 2012; 9(2): 119–20. Russian.

Samokhina LM, Starodub NF, Babiychuk GA, Lomako VV. Rhythmic cold effect on activity of elastases in female rats with alcohol-depended hypertension. Problems of Cryobiology. 2012; 22(1): 49–60. Full Text

Shilo AV, Lutsenko DG, Ventskovskaia EA, et al. [Effect of different types of cold acclimation on osmotic fragility and sphericity index of rat erythrocytes]. Ros Fiziol Zhurn im. I.M. Sechenova 2014; 100(1): 105–11. Russian. PubMed

Siplivy VA, Kon EV, Evtushenko DV. [Use of leukocyte indices for predicting the outcome of peritonitis]. Klіnichna Hіrurgіya. 2009: (9): 21–6. Russian.

Stanek A, Sieroń-Stołtny K, Romuk E, et al. Whole-Body Cryostimulation as an Effective Method of Reducing Oxidative Stress in Healthy Men. Adv Clin Exp Med. 2016; 25(6): 1281– 91.



Szygula Z, Lubkowska A, Giemza C, et al. Hematological parameters, and hematopoietic growth factors: EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students. PLoS ONE [Internet]. 2014 Apr 2 [cited Dec 15 2017]; 9(4): e93096. Evaluable from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0093096. CrossRef PubMed

Turk EE. Hypothermia. Forensic. Sci Med Pathol. 2010; 6(2): 106–15. CrossRef PubMed

Ziemann E, Olek RA, Kujach S, et al. Five-day whole-body cryostimulation, blood inflammatory markers, and performance in high-ranking professional tennis players. J Athl Train. 2012; 47(6): 664–72.



Zubareva EV. [Influence of intensive heat load on morphometric indices and functional activity of leukocytes of rat blood]. Nauchniy rezultat. Seriia Fiziologiya. 2014; (1): 27–34. Russian.

DOI: https://doi.org/10.15407/cryo28.04.293


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