Antioxidant and Antiradical Effects of Extracts Derived From Cryopreserved Human Placenta

Authors

  • Stanislav V. Narozhnyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-9690-0203
  • Kateryna D. Rozanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olena M. Bobrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleg A. Nardid Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo28.04.322

Keywords:

cryopreservation, human placenta extract, antioxidant action, antiradical activity, oxidative stress, sodium nitrite

Abstract

In this study the data concerning placenta low temperature storage impact on antioxidant action of its aque-ous-saline extracts towards erythrocytes under sodium nitrite-induced oxidative stress are provided. The research pro-tocol included two parts: the first one comprised a simultaneous exposure of erythrocytes to the extracts and nitrite, the second one referred to the nitrite addition to the erythrocytes, preliminary treated with the extracts. The level of oxidative stress was assessed by erythrocytes methemoglobin one, antiradical activity was evaluated by the extracts ability to reduce ABTS+ radical. Aqueous-saline extracts keep their protective ability toward erythrocytes under oxidative stress up to three and up to six months for the placenta stored –20°C and –80°C, correspondently. Placenta storage at –20°C for six months led to the lowering of protective effect, as well as antiradical activity down to 50% in comparison with fresh extracts. The 10–12 kDa fraction of the aqueous-saline extracts showed the highest protective effect, which did not change up to three and up to six months for the placenta, stored –20°C and –80°C, respectively. After six months for the placenta stored –20ºC the antiradical activity of this fraction was significantly reduced.

 

Probl Cryobiol Cryomed 2018; 28(4): 322-332

Author Biographies

Stanislav V. Narozhnyi, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Kateryna D. Rozanova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Olena M. Bobrova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Oleg A. Nardid, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

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Published

2019-03-01

How to Cite

Narozhnyi, S. V., Rozanova, K. D., Bobrova, O. M., & Nardid, O. A. (2019). Antioxidant and Antiradical Effects of Extracts Derived From Cryopreserved Human Placenta. Problems of Cryobiology and Cryomedicine, 28(4), 322–332. https://doi.org/10.15407/cryo28.04.322

Issue

Section

Theoretical and Experimental Cryobiology