Experimental Substantiation of Therapeutic Hypothermia and Cell Therapy Application at Dyscirculatory Encephalopathy in SHR Rats. Part 2. Structural Changes in Brain Tissue
DOI:
https://doi.org/10.15407/cryo29.01.058Keywords:
SHR rats, brain, dyscirculatory encephalopathy, craniocerebral hypothermia, cord bloodAbstract
Nowadays the cerebral vascular diseases are recognized as the main causes of disability and mortality, and the issues of optimizing the pathogenetic therapy of this disease remain far from being solved. In this regard, the development of new effective methods to combat the development and progression of cerebrovascular disorders in dyscirculatory encephalopathy (DE) is the most important task of medicine and biology. In this paper, we evaluated the morphological and morphometric parameters of brain tissue in the SHR rats 30 days after applying the rhythmic craniocerebral hypothermia and cryopreserved cord blood nucleated cells. It has been shown that each of the studied methods of influence has a more or less positive effect on reducing the severity of degeneration in the tissue of rat’s brain with the DE signs. The combined use of these methods contributed to their mutual potentiation, manifested in a decrease in the degree of degenerative-dystrophic lesions of brain tissue, as evidenced by a significant decrease in the neuroglial index.Â
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Probl Cryobiol Cryomed 2019; 29(1): 058–072
References
Aidarova VS, Babiichuk VG, Kudokotseva OV, et al. Experimental substantiation of therapeutic hypothermia and cell therapy application at dyscirculatory encephalopathy in SHR rats. Part 1. Spontaneously hypertensive SHR rats as a model of dyscirculatory encephalopathy. Probl Cryobiol Cryomed. 2018; 28(3): 224–36. CrossRef
Aidarova VS, Kudokotseva OV, Lomakin II, Babijchuk GA. Applications of cord blood cells in neurology. Probl Сryobiol Сryomed. 2016; 26(2): 103–15. CrossRef
Amenta F, Di Tullio MA, Tomassoni D. Arterial hypertension and brain damage-evidence from animal models (review). Clin Exp Hypertens. 2003; 25(6): 359–80. CrossRef
Amenta F, Tayebati SK, Tomassoni D. Spontaneously hypertensive rat neuroanatomy: applications to pharmacological research. Ital J Anat Embryol. 2010; 115(1–2): 13–7. PubMed
Babijchuk GA, Marchenko VS, Lomakin II, Belostotskiy AV. [Neurophysiological processes of cooled brain]. Kyiv: Naukova dumka; 1992. Russian.
Babijchuk LA, Kudokotseva OV, Zubov PM, Zubova OL. [New approaches for cryopreservation and estimation of cord blood nucleated cells viability]. Ukrainskiy Khimioterapevticheskiy Zhurnal. 2008; (1–2): 85–8. Russian.
Babijchuk LA, Zubov PM, Ryazantsev VV, et al. [Cord blood as an alternative source of stem cells for regenerative medicine: new approaches to problem of cryopreservation]. Bukovinian Medical Herald. 2009; 13(4): 23–6. Russian.
Babijchuk LV, Koval SN, Babijchuk G.A. Structure of myocardium of young hypertensive rats after injection of cryopreserved human cord blood nucleated cells. Probl Сryobiol Сryomed. 2016; 26(3): 271–87. CrossRef
Chen J, Venkat P, Zacharek A, Chopp M. Neurorestorative therapy for stroke. Front Hum Neurosci [Internet]. 2014 [cited 08.09.2018]; 27(8): 382. Available from: https://www.frontiersin.org/articles/10.3389/fnhum.2014.00382/full
Cheng J, Liu A, Shi MY, Yan Z. Disrupted glutamatergic transmission in prefrontal cortex contributes to behavioral abnormality in an animal model of ADHD. Neuropsychopharmacology. 2017; 42(10): 2096–104. CrossRef
De Deyn PP, Dam DV. Animal models of dementia. NY: Humana Press; 2011. CrossRef
Golovchenko YuI, Treshchinskaya MA. [Pathogenetic features of the development of circulatory brain hypoxia in hypertension]. Meditsyna Neotlozhnykh Sostoyaniy. 2011; 35(4): 86–93. Russian.
Gusev E, Konovalov A, Skvortsova V. [Neurology. National guidelines]. Moscow: GEOTAR; 2009. Russian.
Hachinski V. World stroke day proclamation. Stroke. 2008; 39(9): 2409–20. CrossRef
Lilly R. [Pathohistological technique and practical histochemistry]. Moscow: Mir; 1960. Russian.
Lomakin II. Substantiation of medical cooling methods in therapy of chronic alcoholism. Problems of Cryobiology. 2008; 18(3): 335–8.
Lomakin II, Kudokotseva OV, Purysheva VYu. [Therapeutic effect of cord blood preparations at an example of dermal structural changes under experimental hypothyreosis and its potentiation by aerocryotherapy]. In: Panchenko O.A., editor. [Cryotherapy: safe application technology]. Кyiv: KVIC; 2012. p. 51–9. Russian.
Lomakin II, Shilo OV, Kozlov OV, et al. [Potentiation of tissue therapy effect in the model of pathological aging of the brain in animals]. Transplantologia. 2000; 1(1): 270–1. Ukrainian.
Marchenko VS, Polyschuk LV, Babijchuk VG. Effect of rhythmic cooling on BBB permeability for exogenic norepinephrine. Problems of Сryobiology. 2000; (1): 36–41.
Newman MB, Willing AE, Manressa JJ, et al. Cytokines produced by cultured human umbilical cord blood (HUCB) cells: implications for brain repair. Exp Neurol. 2006; 199(1): 201–8. . CrossRef
O’Brien JT. Vascular cognitive impairment. Lancet Neurology. 2003; 2(2): 89–98. CrossRef
Rahn KY, Barenbrock M, Hausberg M. The sympathetic nervous system in the pathogenesis of hypertension. J Hypertens Suppl. 1999; 17(3): S11–S14. PubMed
Skvortsova VI, Botzina AYu, Koltsova KV. [Arterial hypertension and the brain]. Zhurnal nevrologii i psikhiatrii imeni SS Korsakova. 2006; 10: 68–76. Russian.
Sokolova IB, Polyntsev DG. [Efficacy of mesenchymal stem cells used for the improvement cerebral microcirculation in spontaneously hypertensive rats]. Tsitologiya. 2017; 59(4): 279–84. Russian.
Sokolova IB, Sergeev IV, Fedotova OR, et al. Age-related changes of microcirculation in pia mater of rats’ sensorimotor cortex. Adv Gerontol. 2016; 29(4): 567–72. PubMed
Solovieva AO, Poveshchenko AF, Poveshchenko OV. [Comparative study on the migration and distribution of bone marrow and spleen cells into lymphoid and non-lymphoid organs of CBA mice in vivo in different periods after transplantation]. Bulleten SO RAMN. 2013; 33(4): 35–41. Russian.
Venkat P, Shen Y, Chopp M, Chen J. Cell-based and pharmacological neurorestorative therapies for ischemic stroke. Neuropharmacology. [Internet]. 2018 [cited 08.09.2018]; 134(PtB): 310–22. Available from: https://www.sciencedirect.com/science/article/pii/S0028390817303994?via%3Dihub
Wei L, Fraser JL, Lu ZY, et al. Transplantation of hypoxia preconditioned bone marrow mesenchymal stem cells enhances angiogenesis and neurogenesis after cerebral ischemia in rats. Neurobiol Dis [Internet]. 2012 [cited 08.09.2018]; 46(3): 635–45. Available from: https://www.sciencedirect.com/science/article/pii/S0969996112000733?via%3Dihub
Willing AE, Lixian J, Milliken M, et al. Intravenous versus intrastriatal cord blood administration in a rodent model of stroke. J Neurosci Res. 2003; 73(3): 296–307. CrossRef
Yanhong Z, Honghong Y. Potential therapeutic mechanisms and tracking of transplanted stem cells: implications for stroke treatment. Stem Cells Int. 2017 [Internet]. 2017 [cited 08.09.2018]; (17): Article ID 2707082. Available from: https://www.hindawi.com/journals/sci/2017/2707082/
Yarygin KN, Semchenko VV, Yereniev SI, et al. [Regenerative biology and medicine. Book II. Cellular technologies in therepay of nervous diseases]. Omsk: Omsk Regional Printing House; 2015. 360 p. Russian.
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