Study of Possibility to Obtain and Cryopreserve Adrenal Cell Primary Culture of Newborn Piglets

Authors

  • O. S. Sidorenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. I. Legach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

adrenal fragments, primary culture of adrenal cells, cryopreservation, viability, adhesion

Abstract

There was investigated the effect of method of biological material cryopreservation (composition of cryoprotective medium, cooling rate, cryopreservation of tissue fragments or primary cell culture) on viability of newborn piglets adrenal cells as well as their behavior during further culturing. The cryopreservation method, enabling to achieve a high survival and viability of cells as well as to preserve their ability to adhesion and flattening during further culturing was developed.

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Published

2011-02-21

How to Cite

Sidorenko, O. S., Bozhok, G. A., Legach, E. I., & Gurina, T. M. (2011). Study of Possibility to Obtain and Cryopreserve Adrenal Cell Primary Culture of Newborn Piglets. Problems of Cryobiology and Cryomedicine, 21(1), 58–67. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/155

Issue

Vol. 21 No. 1 (2011): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 O. S. Sidorenko, G. A. Bozhok, E. I. Legach, T. M. Gurina

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