Multiple Injections of Cryopreserved Fetal Liver Cells to Ageing Rats Prevent Age-Related Antioxidant System Changes and Increase Lifespan


  • Olga V. Ochenashko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Yuriy V. Nikitchenko Institute of Biology of the V.N. Karazin Kharkiv National University, Kharkiv
  • Oleksandr Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv



cryopreserved fetal liver cells, multiple injection, rats, ageing, prooxidant-antioxidant potential, lifespan


In the present work, the possibility of using cryopreserved fetal liver cells (ÑFLC) as an anti-aging agent, assessing their effect on the prooxidant-antioxidant balance, the physiological parameters and the life span of aging rats was investigated. It has been established that the repeated administration of ÑFLC (every 3 months) to adult rats from 13 to 22 months of age, prevents changes in the prooxidant-antioxidant balance of the liver and blood that form in older animals by 25 months. Similar multiple injections of ÑFLC to old rats (from 23 months to the end of life) contribute to weight gain, hamper the reduction in the quality of the coat and prolong the life span of experimental rats by 100 days. The results of the work allow considering the transplantation of ÑFLC as a promising approach for correcting the age-related changes associated with the development of oxidative stress.


Probl Cryobiol Cryomed 2019; 29(3): 221–236.

Author Biographies

Olga V. Ochenashko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

Oleksandr Yu. Petrenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry


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How to Cite

Ochenashko, O. V., Nikitchenko, Y. V., & Petrenko, O. Y. (2019). Multiple Injections of Cryopreserved Fetal Liver Cells to Ageing Rats Prevent Age-Related Antioxidant System Changes and Increase Lifespan. Problems of Cryobiology and Cryomedicine, 29(3), 221–236.



Theoretical and Experimental Cryobiology