Histology of Cryopreserved Neonatal Rat Testes After Intratesticular Allotransplantation

Authors

  • Bo Deng Henan University of Science and Technology, Luoyang, PRС
  • Vitaliy Kireev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Kristina Melentyeva SI ‘I.I. Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine’, Kharkiv
  • Igor Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleksandr Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo30.01.034

Keywords:

testis, intratesticular allotransplantation, cryopreservation, immune suppression, immune privilege

Abstract

Abstract: Cryopreservation and transplantation of testicular fragments and cells are utilized in establishing the germplasm bank of endangered species, line-breeding of animals. Moreover, the transplantation of testicular tissue is involved into investigation of the inborn and acquired defects of testicular tissue. Thus the improvement of cryopreservation and transplantation methods is of great interest. Here, we have compared the fi ndings of histological examination of allotransplanted native and cryopreserved neonatal testis. It has been shown that the usage of DMEM/F12 supplemented with 5 mg/ml bovine serum albumin, 0.05 M sucrose, 20 mM Hepes, 1.5 M dimethylsulfoxide as a cryoprotectant, controlled cooling, preservation at -196°С, water bath warming, followed by intra-testicular allotransplantation of neonatal rat testis results in the development of testicular interstitium and spermatogenic epithelium of donor organs into recipient’ body.

 

Probl Cryobiol Cryomed 2020; 30(1): 034–046

Author Biographies

Vitaliy Kireev , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department for the Low Temperature Cryopreservation

Igor Kovalenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department for the Low Temperature Cryopreservation

Oleksandr Pakhomov , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology  

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Published

2020-03-28

How to Cite

Deng , B., Kireev , V., Melentyeva , K., Kovalenko , I., & Pakhomov , O. (2020). Histology of Cryopreserved Neonatal Rat Testes After Intratesticular Allotransplantation. Problems of Cryobiology and Cryomedicine, 30(1), 34-46. https://doi.org/10.15407/cryo30.01.034

Issue

Section

Theoretical and Experimental Cryobiology