Comparative Evaluation of Biological Activity of Fraction Below 5 kDa from Cattle Cord Blood After Low-Temperature Storage (at –80°C) or Lyophilization to Treat Burn Wounds in Rats

Authors

  • Oleksandr Gulevskyy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Natalia Moiseyeva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga Gorina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Andriy Nikolchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Ivan Schenyavsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo30.01.047

Keywords:

low molecular weight fraction, cord blood, low temperature storage, lyophilization, biological activity, burn

Abstract

Abstract: Biological activity of the low molecular weight (down to 5 kDa) fraction from cattle cord blood after storage in lyophilized state at 4°C and –80°C in the form of solution in a model of thermal burn of rats’ skin was investigated. Actovegin with a pronoun-ced wound healing activity was used as a comparator drug. The storage of low molecular weight fraction for 3 months under the chosen conditions was found not to reduce its biological activity. Introduced and lyophilized cord blood fraction to experimen-tal animals after simulation of thermal skin burns contributed to a reduced burn wound area, normalization of leukocyte content and phagocytic activity of neutrophils in peripheral blood. The fi ndings enable considering the lyophilization of the low molecular weight fraction followed by storage at 4°C as sensibly more expedient option of storing and transporting the biologically active substance.

 

Probl Cryobiol Cryomed 2020; 30(1): 047–057

Author Biographies

Oleksandr Gulevskyy, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Natalia Moiseyeva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Olga Gorina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Andriy Nikolchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Ivan Schenyavsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

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Published

2020-03-28

How to Cite

Gulevskyy, O., Moiseyeva, N., Gorina, O., Nikolchenko, A., & Schenyavsky, I. (2020). Comparative Evaluation of Biological Activity of Fraction Below 5 kDa from Cattle Cord Blood After Low-Temperature Storage (at –80°C) or Lyophilization to Treat Burn Wounds in Rats. Problems of Cryobiology and Cryomedicine, 30(1), 47-57. https://doi.org/10.15407/cryo30.01.047

Issue

Section

Theoretical and Experimental Cryobiology