Effect of Electrocoagulational, Cryo- and Ultrasound Treatment of the Gallbladder Bed After Experimental Cholecystectomy


  • Yevgen D Khvorostov V.N. Karazin Kharkiv National University, Kharkiv
  • Sergii O Bychkov V.N. Karazin Kharkiv National University, Kharkiv
  • Olexii I Tsivenko V.N. Karazin Kharkiv National University, Kharkiv
  • Nataliia V. Cherkova V.N. Karazin Kharkiv National University, Kharkiv
  • Liudmyla M. Dushyk V.N. Karazin Kharkiv National University, Kharkiv




hemostasis, cryoeffect, liver, reparative changes, ultrasound exposure, electrocoagulation


The results of electron microscopic examination of reparative processes in liver tissue of rabbits after cholecystectomy performed with monopolar electrocoagulation, ultrasonic scalpel and at low temperatures on days 1, 3, 14, 21 and 30 were comparatively analyzed. Experimental findings allowed to compare the hemo- and cholestatic effects of different types of energy, to assess the degree of trauma to the liver parenchyma in the surgery area and the impact of different methods on the quality and timing of repair. Ultrastructural changes in hepatocytes depending on the methods of influence were characterized with various dystrophic and destructive processes. Electron microscopic studies showed that the ultrastructure of liver cells after electrocoagulation on the bed of the removed gallbladder corresponded the norm on day 30, after cryoeffect it did on day 21, and after ultrasound this was on day 14. It has been proven that the use of ultrasound and cryoeffect in cholecystectomy compared to electrocoagulation occurred to be the least traumatic for liver tissue.


Probl Cryobiol Cryomed 2020; 30(2): 178-187


Aleksandrov VV, Maskin SS, Igolkina LA, et al. [Prospects for the use of local cryohemostasis in cases of liver and splenic trauma]. Kubanskii nauchnyi meditsinskii vestnik. 2013; 142(7): 45−51. Russian.

Astashov VL, Solontsova EN, Mukhtarulina SV. [Application of high technology in liver surgery]. Voen Med Zh. 2013; 334(8): 30−4. Russian. PubMed

Bayzharkinova AB. [Surgical aspects of complications of LCE and TCE]. International journal of applied and fundamental research 2014;12(1): 86−9. Russian.

Bautkin AV, Eleev AA, Melikova SG. [Study of efficaay of ultrasonic scalpel 'UltraÑision Harmonic Scalpel' in the experiment on laboratory animals]. Meditsinskii vestnik Yuga Rossii. 2013; (3): 28−30. Russian.

Boyko VV, Remnyova NA, Chernyayev NS, et al. [Some pathomorphological features of the resection edge of the liver immediately after using the apparatus of high-frequency electrosurgical welding and monopolar electrocoagulator]. Novosti Khirurgii. 2015; 23(3): 256−61. Russian. CrossRef

Melkonyan GG, Mumladze RB. [Using laser technology for laparoscopic cholecystectomy]. Almanakh Instituta khirurgii imeni A.V. Vishnevskogo. 2015; 10(1): 2−3. Russian.

Odishelashvili GD, Ismailov EH. [The choice of ways of hemorrhage stoppage in case of liver damage]. Astrakhanskii meditsinskii zhurnal. 2012; 7(1): 124−6. Russian.

Pakhlevanyan VG, Kolesnikov SA. [Electrode coagulation hemostasis, advantages and disadvantages]. Belgorod State University Scientific bulletin Medicine Pharmacy 2016; 33(5): 5−9. Russian.

Perez Lezana MA, Villarreal CG, Cumplido LP, at al. Comparative study of ambulatory laparoscopic cholecystectomy versus management of laparoscopic cholecystectomy with conventional hospital stay. Cir Esp. 2013; 9(7): 424−31. CrossRef

Solej M, Martino V, Mao P, at al. Early versus delayed laparoscopic cholecystectomy for acute cholecystitis. Minerva Chir. 2012; 67 (5): 381−7.

Sukhіn ІA, Furmanov YuO, Khudetskiy ІYu, et al. [Practical application of a high-frequency coagulators, owing different electrophysical characteristics]. Klіn khіr. 2013; (7): 52−5. Ukrainian.




How to Cite

Khvorostov, Y., Bychkov, S. ., Tsivenko, O. ., Cherkova, N., & Dushyk, L. (2020). Effect of Electrocoagulational, Cryo- and Ultrasound Treatment of the Gallbladder Bed After Experimental Cholecystectomy. Problems of Cryobiology and Cryomedicine, 30(2), 178–187. https://doi.org/10.15407/cryo30.02.178



Cryomedicine, Clinical and Experimental Transplantology