Biologically Active Compositions Containing Neurotrophic Factors Change the Contractile Activity of Detrusor of Rats with Infravesical Obstruction
Keywords:contractile activity, urinary bladder, infravesical obstruction, spinal ganglion, mantle gliocytes, cryoextract, cryopreservation
The contractile activity of urinary bladder (UB) after introduction of biologically active compositions (BAC) containing neurotrophic factors in female rats with infravesical obstruction, which was modeled by a ligature to the urinary tract distal part, was investigated. Cryoextract of the spinal ganglia (SG), conditioned culture media of native and cryopreserved mantle gliocytes (MG), the bulk medium, the drug "Cortexin" were used as BAC. Animals were injected with BAC for 10 days and the contractile activity (CA) of isolated UB strips was determined by measuring KCl-induced and agonist-dependent responses. The use of BAC, in addition to Cortexin, contributed to the CA restoration. The conditioned culture medium of cryopreserved MG and cryoextract of SG increased the agonist-dependent response by 4.6 and 2.5 times, respectively, compared to the CA of the control animals. The rats in these groups showed the highest indices of maximal amplitude of contraction, similar to the control ones.
Probl Cryobiol Cryomed 2020; 30(2): 188-198
Ali S, Bozhok G. Effect of cryopreservation on different passages of porcine dorsal root ganglion cell culture. Recent Adv Biol Med [Internet]. 2019 Nov 2 [cited 2020 Mar 17];5: 941755. Available from: https://rabm.scholasticahq.com/article/10899-effect-of-cryopreservation-on-different-passages-of-porcine-dorsal-root-ganglion-cell-culture CrossRef
AlÑ– SG, Sidorenko ÐžS, Bozhok GÐ. [Influence of nutrient medium composition on the morphological characteristics of culture of dorsal root ganglion cells of neonatal piglets]. The Journal of V. N. Karazin Kharkiv National University. Series Â«BiologyÂ». 2018; 30 (30): 49-59. Ukrainian. CrossRef
Andersson KE. Potential of stem cell treatment in detrusor dysfunction. Adv Drug Deliv Rev. 2015; (82-83): 117-22. CrossRef
Banks FC, Knight GE, Calvert RC, et al. Alterations in purinergic and cholinergic components of contractile responses of isolated detrusor contraction in a rat model of partial bladder outlet obstruction. BJU Int. 2006; 97 (2): 372-8. CrossRef
Barendrecht MM, Chichester P, Michel MC, Levin RM. Effect of short-term outlet obstruction on rat's bladder nerve density and contractility. Auton Autacoid Pharmacol. 2007; 27 (1): 47-53. CrossRef
Baskin LS, Sutherland RS, Thomson AA, et al. Growth factors and receptors in bladder development and obstruction. Lab Invest. 1996; 75 (2): 157-66.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72 (1-2): 248-54. CrossRef
Choppin A. Muscarinic receptors in isolated urinary bladder smooth muscle from different mouse strains. Br J Pharmacol. 2002; 137 (4): 522-8. CrossRef
Cumming JA, Chisholm GD. Changes in detrusor innervation with relief of outflow tract obstruction. Br J Urol. 1992; 69 (1): 7-11. CrossRef
Downi JW, McGuire RP. Antagonism of calcium-induced contraction in potassium-depolarized rabbit detrusor muscle strips by dicyclomine hydrochloride and rodverine. Can J Physiol Pharmacol. 1981; 59 (8): 853-6. CrossRef
Ekman M, Zhu B, SwÃ¤rd K, Uvelius B. Neurite outgrowth in cultured mouse pelvic ganglia - Effects of neurotrophins and bladder tissue. Auton Neurosci. 2017; 205 (1): 41-9. CrossRef
Fry CH. Experimental models to study the physiology, pathophysiology, and pharmacology of the lower urinary tract. J Pharmacol Toxicol Methods. 2004; 49 (3): 201-10. CrossRef
Fusco F, Creta M, De Nunzio C, et al. Progressive bladder remodeling due to bladder outlet obstruction: a systematic review of morphological and molecular evidences in humans. BMC Urol [Internet]. 2018 Mar 09 [Cited 2019 Oct 22]; 18 (1): 15. Available from: https://bmcurol.biomedcentral.com/articles/10.1186/s12894-018-0329-4 CrossRef
Goldman HB, Zimmern PE. The treatment of female bladder outlet obstruction. BJU Int. 2006; 98 (Suppl 1):S17-S23. CrossRef
Gomazkov OA. [Neurotrophic factors of the brain]. Reference-informational edition. [Internet]. Moscow: Institute of Biomedical Chemistry Russian Academy of Medical Sciences; 2004 [cited 2019 Oct 28]. 311 p. Available from: https://www.booksmed.com/biologiya/2166. Russian.
Gomazkov OA. [Neurotrophic therapy and concept of Â«minipeptidesÂ»]. Consilium medicum. 2013; (2): 105-9. Russian.
Juan YS, Chuang SM, Jang MY, et al. Basic research in bladder outlet obstruction. Incon Pelvic Floor Dysfunct [Internet]. 2011 [Cited 2019 Oct 24]; 5 (1):1-6. Available from: http://www.tcs.org.tw/issue/folder/5_1/1-6.pdf.
Kim JC, Yoo JS, Park EY, et al. Muscarinic and purinergic receptor expression in the urothelium of rats with detrusor overactivity induced by bladder outlet obstruction. BJU Int. 2008; 101 (3): 371-5. CrossRef
Kullmann FA, Daugherty SL, de Groat WC, Birder LA. Bladder smooth muscle strip contractility as a method to evaluate lower urinary tract pharmacology. J Vis Exp [Internet]. 2014 Aug 18 [Cited 2019 Oct 24]; (90), e51807. Available from: https://www.jove.com/video/51807/bladder-smooth-muscle-strip-contractility-as-method-to-evaluate-lower CrossRef
Levi-Montalcini R. [Factors of tissue and nerve growth]. Mezhdunarodnyi nevrologicheskii zhurnal [Internet]. 2012 [Cited 28.10.2019]; 3 (49): 29728. Available from: http://www.mif-ua.com/archive/article/29728/. Russian.
Levin R, Chichester P, Levin S, Buttyan R. Role of angiogenesis in bladder response to partial outlet obstruction. Scand J Urol Nephrol Suppl. 2004; 38 (215): 37-47. CrossRef
Levin RM, Longhurst PA, Monson FC, et al. Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder. The Prostate. 1990; 17 (Suppl 3): S9-S26. CrossRef
Levin RM, Ruggieri MR, Velagapudi S, et al. Relevance of spontaneous activity to urinary bladder function: an in vitro and in vivo study. J Urol. 1986; 136 (2): 517-21. CrossRef
Lin WY, Wu CF, Chen CS. Partial bladder outlet obstruction: bladder dysfunction and related issues in animal studies. Urol Sci. 2010; 21 (2): 70âˆ’4. CrossRef
Milicic I, Buckner SA, Daza A, et al. Pharmacological characterization of urinary bladder smooth muscle contractility following partial bladder outlet obstruction in pigs. Eur J Pharmacol. 2006; 532 (1-2): 107-14. CrossRef
Minagawa T, Imamura T, Igawa Y, et al. Differentiation of smooth muscle cells from human amniotic mesenchymal cells implanted in the freeze-injured mouse urinary bladder. European Urology. 2010; 58 (2): 299-306. CrossRef
Zhang NZ, Ma L, Zhang JB, Chen J. Improved model for the establishment and evaluation of detrusor overactivity in female Wistar rats. Int Braz J Urol. 2014; 40 (3): 414-22. CrossRef
Sezginer EK, Yilmaz-Oral D, Lokman U, et al. Effects of varying degrees of partial bladder outlet obstruction on urinary bladder function of rats: A novel link to inflammation, oxidative stress and hypoxia. Low Urin Tract Symptoms. 2019; 11 (2): 193-201. CrossRef
Shavlovskaya OA. [Clinical efficacy of neuropeptides in cerebrovascular pathology]. S.S. Korsakov Journal of Neurology and Psychiatry. 2016; 116 (8): 88-93. Russian. CrossRef
Skaper SD. Neurotrophic Factors. Methods in Molecular Biology (Methods and Protocols). Vol. 846. Totowa, USA: Humana Press; 2012. Chapter 1, The neurotrophin family of neurotrophic factors: an overview; p. 1-12. CrossRef
Speakman MJ, Brading AF, Gilpin CJ, et al. Bladder outflow obstruction-a cause of denervation supersensitivity. J Urol. 1987; 138 (6): 1461-6. CrossRef
Stoll G, MÃ¼ller HW. Nerve injury, axonal degeneration and neural regeneration: basic insights. Brain Pathology. 2006; 9 (2): 313-25. CrossRef
Subota NP, Gryschenko VI, Rozanova KD, inventors; Institute for problems of cryobiology and cryomedicine of the National Academy of Sciences of Ukraine, assignee. [Method for producing fetal preparations]. Patent of Ukraine â„– 36466 A. 2001 April 16. Ukrainian.
Terenghi G. Peripheral nerve regeneration and neurotrophic factors. J Anat. 1999; 194 (1): 1-14. CrossRef
Uvelius B. Isometric and isotonic length-tension relations and variations in cell length in longitudinal smooth muscle from rabbit urinary bladder. Acta Physiol Scand. 1976; 97 (1): 1-12. CrossRef
Vetter I, Pujic Z, Goodhill GJ. The response of dorsal root ganglion axons to nerve growth factor gradients depends on spinal level. J Neurotrauma. 2010; 27 (8): 1379-86. CrossRef
Wein AJ, Rackley RR. Overactive bladder: a better understanding of pathophysiology, diagnosis and management. J Urol. 2006; 175(3 Pt 2): 5-10. CrossRef
Workshop 3: ICS methodology in basic science [Internet]. Tokyo, International Continence Society; 2016 Sept 13. [Cited 2019 Oct 22]. 25p. Available from: https://www.ics.org/Workshops/HandoutFiles/000621.pdf.
Yoshida S, Orimoto N, Tsukihara H, et al. TAC-302 promotes neurite outgrowth of isolated peripheral neurons and prevents bladder denervation related bladder dysfunctions following bladder outlet obstruction in rats. Neurourol Urodyn. 2017; 37 (2): 681-9. CrossRef
Yoshimura N, Ogawa T, Miyazato M, et al. Neural mechanisms underlying lower urinary tract dysfunction. Korean J Urol 2014 ; 55 (2): 81-90. CrossRef
How to Cite
Copyright (c) 2020 Vyacheslav Yu. Globa , Tatyana P. Bondarenko , Galina A. Bozhok , Yana Yu. Samburg , Evgen I. Legach
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).