Impact of Craniocerebral Hypothermia and Cryopreserved Cord Blood on Reproductive Function of Male Rats with Chronic Alcohol Intoxication


  • Іvan І. Lomakin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga V. Kudokotseva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Vladyslav G. Babiichuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Yevhenii V. Kryshtal Kharkiv Medical Academy of Postgraduate Education, Kharkiv



chronic alcohol intoxication, rhythmic craniocerebral hypothermia, cryopreserved cord blood leukoconcentrate, reproductive function, rats


Abstract: In this work, we have studied the impact of chronic alcohol intoxication (CAI) on reproductive function in male rats. Prolonged alcohol abuse has been shown to significantly reduce the manifestation rate of sexual activation and testosterone level in blood serum of male rats with CAI following exposure to a receptive female, as well as to change their seminal fluid composition. The rhythmic craniocerebral hypothermia (rCCH), combined with the administration of cryopreserved cord blood leukoconcentrate (cCBL) ensured to a greater extent the dynamics of functional integrity recovery of the CAI-altered reproductive system in male rats, if compared to each of these methods used solely. The authors have hypothesized the rCCH and cCBL to activate the hypothalamic-pituitary system, affecting thereby the testosterone synthesis and spermatogenesis stimulation.


Probl Cryobiol Cryomed 2020; 30(4): 369–379


Abrahao KP, Salinas AG, Lovinger DM. Alcohol and the brain: neuronal molecular targets, synapses, and circuits. Neuron. 2017; 96 (6):1223-38. CrossRef

Aidarova VS, Babiichuk VG, Kudokotseva OV, et al. Experimental substantiation of therapeutic hypothermia and cell therapy application at dyscirculatory encephalopathy in SHR rats. Part 2. Structural changes in brain tissue. Probl Cryobiol Cryomed. 2019; 29(1): 58-72. CrossRef

Amstislavskaya TG, Osipov KV. [Sexual arousal in the male rat: behavioral and hormonal response]. Byulleten' Sibirskogo Otdeleniya Rossiyskoy Akademii Meditsinskikh Nauk. 2003;(3): 112-4. Russian.

Goltsev АN, Kalinichenko ТА. Human umbilical cord blood as the source of hemopoietic cells for clinical application. Part 1. Characteristics of hemopoietic potential. Problems of Cryobiology. 1998; (1): 3-24.

Grishchenko VI, Koval'ov GA, Petrenko AYu, et al. [Regenerative-plastic therapy of alcoholic visceropathies]. Kyiv: Naukova Dumka; 2010. Russian. 152 p.

Herman M, Lee S, Kang SS, James P. Systemic administration of alcohol to adult rats inhibits leydig cell activity: time course of effect and role of nitric oxide. Alcoholism. Clin Exp Res. 2006; 30(9): 1479-91. CrossRef

Krishtal EV, Marchenko VG, Krishtal TV. [Motivational training as one of the components of psychotherapeutic correction of family health at psychosexual development retardation in men]. Medychna Psykholohiya. 2013; (3): 14-9. Russian.

Krylova EV, Potemina TE, Koryagin AS, Nesterov GA. [Preventive effect of bee royal jelly on rat spermatogenesis indices during acute heat stress]. Bulletin of the Nizhny Novgorod University N.I. Lobachevsky. 2011; 6(1): 138-43. Russian.

Lasek AW. Effects of ethanol on brain extracellular matrix: implications for alcohol use disorder. Alcohol Clin Exp Res. 2016; 40(10): 2030-42. CrossRef

Lee ACK, Ho LM, Yip AWC, et al. The effect of alcohol drinking on erectile dysfunction in Chinese men. Int J Impot Res. 2010; 22: 272-8. CrossRef

Lomakin II. Substantiation of medical cooling methods in therapy of chronic alcoholism. Problems of Cryobiology. 2008; 18(3): 383-5.

Lomakin II, Babiychuk GA. Use of medical cooling methods against chronic alcohol intoxication. Problems of Cryobiology. 2012; 22(3): 355.

Martynova YuV, Babiychuk VG, Sirotenko LA, et al. Neurohumoral changes in rats of diff erent age groups on the background of injection of cryopreserved cord blood nucleated cells. Adv Gerontol. 2016; 6(4): 304-10. CrossRef

Pendharkar S, Mattoo SK, Grover S. Sexual dysfunctions in alcohol-dependent men: a study from north India. Indian J Med Res. 2016; 144(3): 393-9. CrossRef

Ponizovsky AM. Clinical and psychosocial factors associated with quality of life in alcoholdependent men with erectile dysfunction. J Sex Med. 2008; 5(10): 2347-58. CrossRef

Potyomina TE, Kuznetsova SV, Lyalyaev VA. [Alteration of the white rat male seminal fluid parameters at different types of experimental stress]. Sovremennyye Tekhnologii v Meditsine. 2009; (2): 23-6. Russian.

Rachdaoui N, Sarkar DK, Phil D. Pathophysiology of the effects of alcohol abuse on the endocrine system. Alcohol Res. 2017; 38(2): 255-76.

Rasmussen DD, Wilkinson CW, Raskind MA. Chronic daily ethanol and withdrawal: long-term changes in plasma testosterone regulation, but no effect on GnRH gene expression or plasma LH concentrations. Endocrine. 2003; 22(2): 143-50. CrossRef

Romanov YuA, Romanov AYu. [Tissues of perinatal origin is a unique source of cells for regenerative medicine. Part I. Cord blood]. Neonatology: News, Opinions, Training. 2018; 6(2): 64-77. Russian.

Roney JR, Lukaszewski AW, Simmons ZL. Rapid endocrine responses of young men to social interactions with young women. Horm Behav. 2007; 52(3): 326-33. CrossRef

Sansone A, Di Dato C, de Angelis C, et al. Smoke, alcohol and drug addiction and male fertility. Reprod Biol Endocrinol [Internet]. 2018 Jan 18 [cited 2020 Jun 11]; 16(1): 3. Available from: 018-0320-7 CrossRef

Siembida J, Frończuk P, Morylowska-Topolska J, et al. An overlooked issue: sexual dysfunctions in men addicted to alcohol. Curr Probl Psychiatry 2018; 19(2): 112-24. CrossRef

Schill WB, Comhaire FN, Hargreave TB. Andrology for the clinician. Berlin, Heidelberg: Springer-Verlag; 2011.

Shim J-H, Kim Y-T, Kim S, Baek H-M. Volumetric reductions of subcortical structures and their localizations in alcohol-dependent patients. Front Neurol. [Internet]. 2019 Mar 19 [cited 2020 Jun 11]; 10: 247. Available from: https://www.frontiersin. org/articles/10.3389/fneur.2019.00247/full CrossRef

Tikhonova MA, Amstislavskaya TG. [Phenotyping the males of mouse and rat strains with genetically defined behavioral disturbances in a model of sexual activation]. Vavilovskii Zhurnal Genetiki i Selektsii. 2015; 19(4): 413-9. Russian. CrossRef

Tsutsaeva AO, Grischenko VI, Kudokotseva OV, et al. [Procurement, cryopreservation and clinical application of hemopoietic cells of human cord blood]. Methodical Recommendations. Kharkiv; 2000. Russian. 16 p.

Weber MF, Smith DP, O'Connell DL, et al. Risk factors for erectile dysfunction in a cohort of 108 477 Australian Men. Med J Aust. 2013; 199: 107-11. CrossRef




How to Cite

Lomakin І., Kudokotseva , O., Babiichuk , V., & Kryshtal , Y. (2020). Impact of Craniocerebral Hypothermia and Cryopreserved Cord Blood on Reproductive Function of Male Rats with Chronic Alcohol Intoxication. Problems of Cryobiology and Cryomedicine, 30(4), 369–379.



Cryomedicine, Clinical and Experimental Transplantology