Use of Modern Cryomethods in Combined Surgical Treatment of Patients with Purulent Chronic Wounds

Authors

  • Kyrylo A. Goltsev Kharkiv National Medical University, Kharkiv;
  • Igor A. Kryvoruchko Kharkiv National Medical University, Kharkiv
  • Аnatoliy М. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center of the National Academy of Sciences of Ukraine, Academy of Medical Sciences of Ukraine and Ministry of Health of Ukraine, Kharkiv https://orcid.org/0000-0002-5289-5876
  • Kyrylo Yu. Parkhomenko Municipal Health Care Institution ‘Regional Clinical Hospital - Center for Emergency Care and Disaster Medicine’, Kharkiv
  • Eduard M. Horoshun Military Medical Clinical Center of the Northern Region of the Ministry of Defense of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo31.02.109

Keywords:

purulent wounds, combined surgical treatment, cryopreserved cord blood serum, vacuum therapy

Abstract

The paper considers the possibilities of using in medical practice various cryomethods and techniques of cryopreservation of biological objects to treat the patients with purulent chronic wounds (PCW). The variety of methods and approaches to apply cold in medical practice: general and local cryotherapy, cryosurgery etc. has been emphasized. As a result of using each of these methods, regenerative processes begin, which promote the healing of PCW. Particular attention is paid to cryotechniques, ensuring the preservation of cord blood at low temperatures. Cord blood cells and serum have been shown to have immune modulatory and trophic-stimulating therapeutic effects, that is extremely important when treating the patients with PCW. The issue of combined use of cord blood serum and innovative vacuum therapy for the patients with PCW has been considered.

Probl Cryobiol Cryomed 2021; 31(2): 109–115

Author Biography

Аnatoliy М. Goltsev , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center of the National Academy of Sciences of Ukraine, Academy of Medical Sciences of Ukraine and Ministry of Health of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

References

Abdel-Meguid AM, Weshahy AH, Sayed DS, et al. Intralesional vs. contact cryosurgery in treatment of keloids: a clinical and immunohistochemical study. Int J Dermatol. 2015; 54(4): 468- 75. CrossRef

Anatichuk LI, Denisenko OI, Kobylianskiy RR, Kadinuk TY. [About using thermoelectrical cooling in dermatology and cosmetology]. Thermoelectric. 2015; (3): 57-71. Ukranian.

Babaeva AG. [Past, present, and future problems in lymphoid regulation of nonlymphoid cell proliferation]. Biull Eksp Biol Med. 1995 Sep;120(9): 230-4. Russian. CrossRef

Babiychuk VG, Ershova OV, Malysheva GV, et al. Clinical application of rhythmic hypothermia. Problems of Cryobiology. 2002; (1): 85-92.

Eve DJ, Ehrhart J, Zesiewicz T, et al. Plasma derived from human umbilical cord blood modulates mitogen-induced proliferation of mononuclear cells isolated from the peripheral blood of ALS patients. Cell Transplant. 2016; 25(5): 963-71. CrossRef

Gavrilenko AV, Voronov DA. [Results of comprehensive management of patients with chronic lower limb ischaemia using gene-engineering technologies of angiogenesis stimulation (part 1)]. Angiology and vascular surgery. 2015; 21(3): 7-15. Russian.

Gavrilenko AV, Voronov DA. [Results of comprehensive management of patients with chronic lower limb ischaemia using gene-engineering technologies of angiogenesis stimulation (part 2)]. Angiology and vascular surgery. 2015; 21 (4): 29-34. Russian.

Goltsev AN, Yurchenko TN, editors. [Placenta: cryopreservation, clinical use: monograph]. Kharkiv: Brovin AV; 2013. 318 p. Russian.

Goltsev KA, Parkhomenko KIu, Kryvoruchko IA, Shevchenko OM, Azhgybesov KA, Bondarovych MO, Ostankov MV, Goltsev AM. inventors; Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, assignee. [A method of treating acute purulent peritonitis]. Patent of Ukraine N 139447. 2020 Jan 01. Ukrainian.

Goltsev KA, Volina VV, Kozhina OYu, Ostankov MV. Effect of cryopreserved cord blood on structure of immunocompetent and other vital organs at acute pyoperitonitis. Problems of Cryobiology. 2011; 21(4): 429-44. Full Text

Grischenko VI, Prokopyuk OS. Lipina OV. [Procurement, cryopreservation of serum and plasma of cord blood and their clinical use. Methodical recommendations]. Kharkiv; 2000. 11 p. Ukranian.

Grishchenko VI, Goltsev AN. Transplantation of products of embryo-placental complex. From understanding the mechanism of action to improving the effectiveness of the application. Problems of Cryobiology. 2002; (1): 54-84.

Grischenko VI, Krivoruchko IA, Goltsev KA, et al. [Correction of immune impairments by cryopreserved cord blood use during development of acute pyoperitonitis]. Medicine Today and Tomorrow. 2011; (3): 53-62. Russian.

Ischenko IO, Tynynyka LN, Kovaliev GA, et al. [Effect of cryopreserved cord blood serum on biochemical markers of destruction of tissues]. Experimental and clinical medicine. 2016; (1): 19-25. Russian.

Ivanova AA, Antonova AA, Fynageev DM, et al. [Trophic ulcer is a problem of modern surgery]. Youh Scientific Forum: Natural and Medical Sciences. 2017; (5): 93-8. Russian.

Khiao IM, Wallmeyer L, Hedtrich S, et al. The effect of endothelialization on the epidermal differentiation in human three-dimensional skin constructs - a morphological study. Clin Hemorheol Microcirc. 2015;61(2):157-74. CrossRef

Koval AK, Lutsenko ED, Grisha IG, et al. Impact of lyophilisation on integrity of structural and functional characteristics of human cord blood leukoncentrate. Probl Cryobiol Cryomed. 2019; 29 (4): 333-43. CrossRef

Kovalev GA, Vysekantsev IP, Ischenko IO, et al. Effect of cryopreserved cord blood serum and placental extract on cold wound healing. Probl Cryobiol Cryomed. 2015; 25(1): 57-66. CrossRef

Lipina OV, Prokopyuk OS, Savchenko YuA. To the question about cord blood plasm cryopreservation. Problems of Cryobiology. 2000; (4): 83-4.

Olefirenko AA, Tynynyka LN, Sandomirsky BP. Combined usage of cryodestruction and xenoextract to stimulate liver regeneration in rats with experimental cirrhosis. Problems of Cryobiology. 2006; 16(1): 94-100. Full Text

Patel K, Bakshi N, Freehill MT, Awan TM. Whole-body cryotherapy in sports medicine. Curr Sports Med Rep. 2019; 18(4): 136-40. CrossRef

Popovych YaO. Cryopreserved cord blood serum preparation Cryocord-C in treatment of diabetic foot syndrome. Problems of Cryobiology. 2005. 15(1): 63-70. Full Text

Posypenko OB, Stelmakh GO, Bakaluk TG. [Effectiveness of local cryotherapy in treatment of musculoskeletal disorders]. Nursing. 2020; (3): 35-7. Ukranian. CrossRef

Razmakhnin EV, Shangin VA, Kudryavtseva OG, Okhlopkov DY. [Possibilities of vacuum-instillation therapy with dimexidum and betadine in the treatment of purulent wounds]. Acta Biomedica Scientifica (East Siberian Biomedical Journal). 2017;2(6): 153-6. Russian. CrossRef

Sandomirsky BP, Volkova NA. [Skin viability during cryopreservation]. Kyiv: Naukova Dumka; 1985. 87 p. Russian.

Siqueira AF, Vieira A, Ramos GV, et al. Multiple cryotherapy applications attenuate oxidative stress following skeletal muscle injury. Redox Rep. 2017; 22(6): 323-9. CrossRef

Skrzep-Poloczek B, Romuk E, Wisnowiska B, et al. Effect of whole-body cryotherapy on antioxidant systems in experimental rat model. Oxid Med Cell Longev. [Internet]. 2017 Jun 27 [cited 2021 February 12]; 2017;2017:8158702. Available from: https://www.hindawi.com/journals/omcl/2017/8158702/ CrossRef

Sugasawa T, Tome Y, Takeuchi Y, et al. Influence of intermittent cold stimulations on CREB and its targeting genes in muscle: investigations into molecular mechanisms of local cryotherapy. Int J Mol Sci. [Internet]. 2020 Jun 28 [cited 2021 February 12]; 21(13): 4588. Available from: https://www.mdpi.com/1422-0067/21/13/4588 CrossRef

Tettelbach W, Cazzell S, Sigal F, et al. A multicentre prospective randomised controlled comparative parallel study of dehydrated human umbilical cord (EpiCord) allograft for the treatment of diabetic foot ulcers. Int Wound J. 2019; 16(1): 122-30. CrossRef

Vlasov OO, Ishchenko IO, Kovalov GA, et al. Wound repair with cryopreserved cord blood serum treatment In: Proceedings of the Central European conference on regenerative medicine, 2015, March 14-15 Bydgoszcz, Poland. Bydgoszcz; 2015. p. 50-1.

Zembron-Lacny A, Morawin B, Wawrzyniak-Gramacka E, et al. Multiple cryotherapy attenuates oxi-inflammatory response following skeletal muscle injury. Int J Environ Res Public Health. [Internet]. 2020 Oct 27 [cited 2021 February 12]; 17(21): 7855. Available from: https://www.mdpi.com/1660-4601/17/21/7855. CrossRef

Zouboulis CC, Wild T, Zouboulis VA, Har-Shai Y. Intralesional cryosurgery of keloids: required treatment hold time. Br J Dermatol. 2021; 184(1):173-5. CrossRef

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Published

2021-07-15

How to Cite

Goltsev, K., Kryvoruchko, I., Goltsev А., Parkhomenko, K., & Horoshun, E. (2021). Use of Modern Cryomethods in Combined Surgical Treatment of Patients with Purulent Chronic Wounds. Problems of Cryobiology and Cryomedicine, 31(2), 109–115. https://doi.org/10.15407/cryo31.02.109