Impact of Biologically Active Complexes of L. rhamnosus GG and S. boulardii After Storage Under Low Temperatures on Biofilm Forming Ability in Corynebacteria

Authors

  • Olena Yu. Isayenko Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Oksana V. Knysh Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Mykola M. Popov Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Valeriy V. Minukhin Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Eugeny M. Babych Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Olena G. Peretyatko Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo31.02.127

Keywords:

corynebacteria, biofilm, biologically active complexes, Lactobacillus rhamnosus GG, Saccharomyces boulardii, antibiofilm formation activity, low temperature regimens of storage

Abstract

This paper describes the temperature regimens and shelf life of biologically active complexes (structural components and metabolites) of Lactobacillus rhamnosus GG and Saccharomyces boulardii, which have antimicrobial activity and reduce biofi lm formation in pathogenic agents of Corynebacterium spp. Preservation of biological activity of complexes after 6-month storage (observation period) at (−23 ± 1)°C and for 60 days (observation period) under hypothermia at (4 ± 1)°C has been demonstrated. The degree of inhibition of biofi lm formation in pathogenic corynebacteria depended on sensitivity of the Corynebacterium spp. test-culture strain to products of microbial origin. A mixture of lactobacillus and saccharomycete metabolites displayed weak biofi lm formation by toxicogenic strains of corynebacteria (p < 0.05). Our fi ndings testifi ed to a possible use of biologically active substances, stored at 4 and −23°C in designing the L. rhamnosus GG and S. boulardii complexes and in technological processes of their production to prevent the persistence of the diphtheria pathogens.

Probl Cryobiol Cryomed 2021; 31(2): 127–138

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Published

2021-07-15

How to Cite

Isayenko, O., Knysh, O., Popov, M., Minukhin, V., Babych, E., & Peretyatko, O. . (2021). Impact of Biologically Active Complexes of L. rhamnosus GG and S. boulardii After Storage Under Low Temperatures on Biofilm Forming Ability in Corynebacteria. Problems of Cryobiology and Cryomedicine, 31(2), 127–138. https://doi.org/10.15407/cryo31.02.127

Issue

Section

Theoretical and Experimental Cryobiology