Blood Characteristics in Dogs During Treatment of Babesiosis Using Transfusion of Cryopreserved Erythrocytes

Authors

  • Оlha M. Denysova State Biotechnological University, Institute of Veterinary Medicine and Animal Husbandry
  • Gennadiy F. Zhegunov State Biotechnological University, Institute of Veterinary Medicine and Animal Husbandry https://orcid.org/0000-0002-7224-0375
  • Tatyana I. Yakimenko State Biotechnological University, Institute of Veterinary Medicine and Animal Husbandry
  • Nataliia I. Hladka State Biotechnological University, Institute of Veterinary Medicine and Animal Husbandry
  • Vita O. Prichodchenko State Biotechnological University, Institute of Veterinary Medicine and Animal Husbandry
  • Olha M. Bobrutska State Biotechnological University, Institute of Veterinary Medicine and Animal Husbandry

DOI:

https://doi.org/10.15407/cryo31.04.304

Keywords:

babesiosis, canine erythrocytes, transfusion, cryopreservation, cryoprotectant

Abstract

Babesiosis is one of the most common protozoan diseases in dogs, which in the absence of the necessary veterinary care often leads to the animal death. The changes of hematological parameters in dogs during complex treatment of babesiosis and transfusion of cryopreserved erythrocytes were evaluated in the research. Erythrocytes were frozen using 20% DMSO by immersion into liquid nitrogen. Transfusion of cryopreserved erythrocytes together with antiparasitic chemotherapy has been shown to promote rapid recovery of hematological parameters of animal blood (hemoglobin, hematocrit and erythrocyte counts), as well as improves their well-being, promotes faster recovery. Cryopreserved with DMSO erythrocytes can be recommended for long-term storage of blood at cryobanks.

 

Probl Cryobiol Cryomed 2021; 31(4): 304–315

References

Aktaran Bala D, Özcan M. The effects of freezing on long-term storage of canine erythrocytes. Pol J Vet Sci. 2016; 19(2):401-6. CrossRef

Contreras TJ, Lindberg JR, Lowrie GB, et al. Liquid and freeze-preservation of dog red blood cells. Transfusion. 1979; 19 (3): 279-92. CrossRef

Соtter S.M. Advances in veterinary science and comparative medicine: comparative transfusion medicine, V. 36. San Diego: Academic Press; 1991. 343 p.

DeLuca LA, Glass SG, Johnson RE, Burger M. Description and evaluation of a canine volunteer blood donor program. J Appl Anim Welf Sci. 2006; 9 (2): 129-41. CrossRef

Denisova ON, Zhegunov GF, Babijchuk LA. Cryopreservation of animal's erythrocytes under dimethyl sulfoxide, polyethylene oxide and glycerol protection. Problems of Cryobiology. 2005; 15 (2): 195-201. Full Text

Denysova ON, Zhegunov GF, Zemlianskykh NG. [Resistance of erythrocytes of animals against the influence of low temperatures.] Naukovyi Vіsnyk Natsіonalnogo Agrarnogo Unіversytetu. 2008; 126: 67-75. Ukraine

Dobry E, Novac J. Hematologie a transfuzni sluzba, Zdravotnicke actuality 211, Praha: Avicenum; 1987. 188 p.

Graham JE, Meola DM, Kini NR, Hoffman AM. Comparison of the effects of glycerol, dimethyl sulfoxide, and hydroxyethyl starch solutions for cryopreservation of avian red blood cells. Am J Vet Res. 2015; 76 (6): 487-93. CrossRef

Kim H, Itamoto K, Une S, et al. Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch. CryoLetters. 2005; 26 (1): 1-6. PubMed

Kim H, Tanaka S, Une S, et al. A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation. J Vet Med Sci. 2004; 66 (12): 1543-7. CrossRef

Kostyaev AA, Martusevich AK, Andreev AA. [Toxicity of cryoprotectants and cryoconservants on their basis for blood components and bone marrow]. Nauchnoe obozrenie. Meditsinskie nauki.2016; (6): 54-74. Russian.

Langer R, Albrecht R, Hempel K, et al. Characterization of 24-hour survival rate and duration of survival of hydroxyethyl starch cryopreserved erythrocytes after autologous transfusion in the dog. Infusionsther Transfusionsmed. 1994; 21 (6): 393-400. CrossRef

Leisewitz AL, Guthrie AJ, Berry WL. Evaluation of the effect of whole - blood transfusion on the oxygen status and acid-base balance of Babesia canis infected dogs using the oxygen status algorithm. J S Afr Vet Assoc. 1996; 67 (6): 20-6.

Meyer D, Kharvy D. [Veterinary laboratory medicine. Interpretation and diagnosis] Moskow: Sofyon; 2007. 456. Russian.

Pogozhykh D, Pakhomova Y, Pervushina O, et al. Exploring the possibility of cryopreservation of feline and canine erythrocytes by rapid freezing with penetrating and non-penetrating cryoprotectants. PLoS One One [Internet]. 2017 Jan 10 [cited 2020 May 29]; Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169689 CrossRef

Rozanski E, de Laforcade AM. Transfusion medicine in veterinary emergency and critical care medicine. Clin Tech Small Anim Pract. 2004; 19: 83-7. CrossRef

Sant'Anna Leal PD, Flausino W, Gomes Lopes CW. [Diagnosis of concomitant infections due to Neospora caninum, Babesia canis and Erhlichia spp. in adult canine Golden Retriever breed - Case report]. Rev Brasileira de Med Vet. 2012; 34 (1): 47-51. Portuguese.

.

Singbartl K, Langer R, Henrich A. Altered membrane skeleton of hydroxyethylstarch-cryopreserved human erythrocytes. Cryobiology. 1998; 36 (2): 115-23. CrossRef

Smirnov AA, Fedosov AA, Klimov PV. [Drug Pyro-stop - present and effective solution in the fight against animal piroplasmidosis.] Voprosy' normativno-pravovogo regulirovaniya v veterinarii. 2011; 1: 45-7. Russian.

Sputtek A, Langer R, Schmid H, et al. Cryopreservation of erythrocytes with hydroxyethyl starch. In vitro results leading to an autologous retransfusion model in the dog. Beitr Infusionsther. 1992; 30: 292-6.

Swanson BN. Medical use of dimethyl sulfoxide (DMSO).Rev Clin Basic Pharm. 1985; 5(1-2):1-33.

Wardrop KJ, Owen TJ, Meyers KM. Evaluation of an additive solution for preservation of canine red blood cells. J Vet Intern Med. 1994; 8: 253-7. CrossRef

Weiss DJ, Wardrop KJ, editors. Schalm's veterinary hematology. Singapore: Wiley; 2010. 1232 p.

Wintrobe MM. Principles of hematologic examination. In: Wintrobe MM, editor. Clinical hematology. 8th ed. Philadelphia: Lea & Febiger; 1981. p.7-19.

Wong LK, Reinertson EL. Clinical considerations of dimethyl sulfoxide. Iowa State University Veterinarian. 1984; 46 (2): 89-95.

Zemlianskykh NG, Babijchuk LA. [Spatial-conformational modifications of proteins in membrane]. Biologіscheskie membrany.2019;36 (2): 125-36. Russian. CrossRef

Zemlianskykh NG, Denisova ON. [Changes in erythrocyte membrane-cytoskeleton complex, induced by dimethylsulfoxide, polyethyleneglycol, and low temperature]. Biofizika. 2009; 54 (4): 490-6. Russian. CrossRef

Zhegunov GF, Denisova ON. [Permeability of mammalian erythrocytes for the molecules of glycerol and DMSO and the level of cellular viability after freezing-thawing.] Dopov Nac akad nauk Ukr. 2010; 12: 139-43. Russian.

Downloads

Published

2021-12-24

How to Cite

Denysova О., Zhegunov, G., Yakimenko , T., Hladka, N., Prichodchenko, V., & Bobrutska, O. (2021). Blood Characteristics in Dogs During Treatment of Babesiosis Using Transfusion of Cryopreserved Erythrocytes. Problems of Cryobiology and Cryomedicine, 31(4), 304–315. https://doi.org/10.15407/cryo31.04.304

Issue

Section

Theoretical and Experimental Cryobiology