Osmotic Characteristics of Erythrocytes After Freezing with Composition-Modified Cryopreservatives

Authors

  • Viktor V. Ramazanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Yelyzaveta L. Volovelska Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Valeriy A. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Keywords:

erythrocytes, freezing, glycerol, 1,2-propanediol, osmotic stress

Abstract

Here, we have studied the osmotic parameters of erythrocytes frozen in the composition-modified media with glycerol and 1,2-propanediol. A decreased glycerol concentration in a cryopreservative and an increased content of impermeable protective component sorbitol were established to affect the erythrocyte characteristics at different stages of freezing and hypothermic storage. Reduction of 1,2-propanediol and NaCl concentrations when augmenting the sucrose content in cryopreservative agent enabled changing a two-stage mode of erythrocyte freeze-thawing to the single-stage one. The presented here modification of cryoprotective medium allowed to diminish the level of damage to erythrocytes during freeze-thawing-washing out, that might prevent the inflammation development in a body when transfusing these cells.

Probl Cryobiol Cryomed 2022; 32(2):092103

Author Biographies

Viktor V. Ramazanov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Yelyzaveta L. Volovelska, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Valeriy A. Bondarenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

References

Bakhach J. The cryopreservation of composite tissues: Principles and recent advancement on cryopreservation of different type of tissues. Organogenesis. 2009; 5(3): 119-26. CrossRef

Bogner V, Keil L, Kanz KG, et al. Very early posttraumatic serum alterations are significantly associated to initial massive RBC substitution, injury severity, multiple organ failure and adverse clinical outcome in multiple injured patients. Eur J Med Res. 2009; 14(7): 284-91. CrossRef

De Loecker R, Goossens W, Van Duppen V. Osmotic effects of dilution on erythrocytes after freezing and thawing in glycerol-containing buffer. Cryobiology. 1993; 30(3): 279-85. CrossRef

Guchok VM, Vorotilin AM, Lougovov VI, Shrago MI. Therapeutic efficiency of RBC, cryopreserved under protection of a "Propanediosaccharole" preparation. Problems of Cryobiology. 1994; (4): 43-6.

Henkelman S, Noorman F, Badloe JF, Lagerberg JW. Utilization and quality of cryopreserved red blood cells in transfusion medicine. Vox Sang. 2015; 108: 103-12. CrossRef

Hod EA, Zhang N, Sokol SA, et al. Transfusion of red blood cells after prolonged storage produces harmful effects that are mediated by iron and inflammation. Blood. 2010; 115(21): 4284-92. CrossRef

Lelkens CC, Noorman F, Koning JG, et al. Stability after thawing of RBCs frozen with the high- and low-glycerol method. Transfusion. 2003; 43(2): 157-64. CrossRef

Miller RH, Mazur P. Survival of frozen-thawed human red cells as a function of cooling and warming velocities. Cryobiology. 1976; 13(4): 404-14. CrossRef

Pakhomova YuS, Kompaniets AM, Kuleshova LG. Transformation of erythrocytes during cryopreservation with oxyethylated glycerol derivatives with n=25 and n=30 polymerization degree. Probl Сryobiol Cryomed. 2016; 26(4): 349-60. CrossRef

Ramazanov VV. Effect of combined media on damage of erythrocytes, frozen with different hematocrit values. Problems of Cryobiology. 2006; 16(2): 155-63. Full Text

Ramazanov VV, Volovelska YeL, inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [The method of cryopreservation of human erythrocytes]. Patent of Ukraine №114838A. March 27 2017. Ukrainian.

Rall WF, Mazur P, Souzu H. Physical-chemical basis of the protection of slowly frozen human erythrocytes by glycerol. Biophys J. 1978; 23(1):101-20. CrossRef

Spitalnik SL. Stored red blood cell transfusions: iron, inflammation, immunity, and infection. Transfusion. 2014; 54(10): 2365-71. CrossRef

Thuret I. Post-transfusional iron overload in the haemoglobinopathies. C R Biol. 2013; 336(3): 164-72. CrossRef

Valeri CR, Ragno G. An approach to prevent the severe adverse events associated with transfusion of FDA-approved blood products. Transfus Apher Sci. 2010; 42(3): 223-33. CrossRef

Downloads

Published

2022-10-30

How to Cite

Ramazanov, V., Volovelska, Y., & Bondarenko , V. (2022). Osmotic Characteristics of Erythrocytes After Freezing with Composition-Modified Cryopreservatives . Problems of Cryobiology and Cryomedicine, 32(2), 104–110. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1791

Issue

Section

Cryopreservation of Biological Resources