Pituitary-Thyroid System in Rats of Different Ages Under Desynchronosis, Whole-Body Cryostimulation, and Cord Blood Injection
Keywords:thyroid-stimulating hormone, thyroid hormones, desynchronosis, cryostimulation, cryopreserved cord blood, age
The state of the pituitary-thyroid system central and peripheral links (according to the levels of thyroid stimulating hormone (TSH) and thyroid hormones T4 and T3 in blood serum) were studied in 6- and 18-month-old rats under initiated circadian desynchronosis (CD) by the light regime inverting. The CD was simulated against the background of separate and combined eff ect of both the whole body cryostimulation (–120°C) (WBC) and injections of the nucleated cells (NCs) of human cord blood, applied preventively (for a day and week, respectively). In 6-month-old rats, the TSH level decreased under the CD and its initiation after separate NCs administration and combined with WBC (by 3.4, 3.9 and 6.3 times, respectively) and remained unchanged under CD against the WBC. In 18-month-old rats the TSH level was reduced only at CD by 1.8 times. The T4 level in rats of both age groups did not change under the CD as well as during its development against the background of a combined effect of WBC and NCs administration in 6-month-old animals and against the NCs injection in 18 months-old rats. The T4 level in rats of both age groups decreased at the background of separate effect of the WBC (by 1.6 and 1.4 times, respectively). In 18-month-old animals, the T4 increased under the CD development after a combined effect of WBC and NCs injection by 1.3 times. No changes in the T3 level were found.
Probl Cryobiol Cryomed 2022; 32(3):196–205
Babiychuk VG, Kozlov OV, Lomakin II, Babiychuk GO, inventors; Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, assignee. [Cryochamber for experimental cooling of laboratory animals]. Ukraine patent № 40168. 2009 March 25. Ukrainian.
Babijchuk LO, Grishchenko VI, Gurina TM, et al., inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [The method of cryopreservation of nucleated cord blood cells, including hematopoietic stem cells]. Patent of Ukraine N 92227. 11.10.2010. Ukrainian.
Berglund S, Magalhaes I, Gaballa A, et al. Advances in umbilical cord blood cell therapy: the present and the future. Expert Opin Biol Ther. 2017: 17(6):691–9. CrossRef
Castellano JM, Mosher KI, Abbey RJ, et al. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature. 2017; 544 (7651): 488–92. CrossRef
Chernyavskaya EA, Volina VV, Babiichuk VG. Structure of myocardium of young rats with simulated alimentary obesity following the application of combined rhythmic extreme cold effects and cryopreserved cord blood nucleated cells. Probl Cryobiol Cryomed 2018; 28(2): 108–19. CrossRef
Douzi W, Dupuy O, Theurot D, et al. Partial body cryostimulation after training improves sleep quality in professional soccer players. BMC Res Notes [Internet]. 2019 15 Mar [cited 20.05.2021]; 12:141. Available from: https://bmcresnotes.
Fahrenkrug J, Georg B, Hannibal J, Jørgensen HL. Hypophysectomyabolishes rhythms in rat thyroid hormones but not in the thyroid clock. Endocrinol. 2017; 233(3):209–16. CrossRef
Gubin DG. [Molecular basis of circadian rhythms and principles of circadian disruption] Usp Fiziol Nauk. 2013; 44(4): 65–87. Russian. PubMed
Hansen J. Night shift work and risk of breast cancer. Curr Environ Health Rep. 2017; 4 (3): 325–39. CrossRef
Ikegami K, Refetoff S, Van Cauter E, Yoshimura T. Interconnection between circadian clocks and thyroid function. Nat Rev Endocrinol. 2019; 15(10): 590–600. CrossRef
Lomako VV, Shylo AV, Babijchuk GA, Samokhina LM. Craniocerebral hypothermia stimulates reactions of limited proteolysis in rat tissues. Probl Cryobiol Cryomed 2016; 26(3): 238–48. CrossRef
Lombardi G, Ziemann E, Banfi G. Whole-body cryotherapy in athletes: from therapy to stimulation. An updated review of the literature. Front Physiol [Internet]. 2017 May 2 [cited 20.05.2021]; 8: 258. Available from: https://www.frontiersin.org/
Peeters RP. Thyroid hormones and aging. Hormones. 2008; 7(1): 28–35.
Puttonen S, Oksanen T, Vahtera J, et al. Is shift work a risk factor for rheumatoid arthritis? The Finnish public sector study. Ann Rheum Dis. 2010; 69(4): 779–80. CrossRef
Sholohov LF, Kolesnikova LI, Chudinova EL, et al. [Evaluation of the functional state of the pituitary-thyroid system in women of reproductive age with acute hepatitis A]. Fundamentalnyye issledovaniya. 2014; (10, Part 2): 390–3. Russian.
Sudo A, Miki K. Circadian rhythm of catecholamine excretion in rats after phase shift of light-dark cycle. Indust Health. 1995; 33 (2): 57–66. CrossRef
Villeda SA, Plambeck KE, Middeldorp J, et al. Young blood reverses age-related impairments in cognitive function and synaptic plasticity in mice. Nat Med. 2014; 20(6):659–63. CrossRef
How to Cite
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).