Morphogenetic Responses of Triticum aestivum L. Calus Culture During Long-Term Effect of Low Positive Temperatures




Triticum aestivum L., in vitro callus culture, VRN genes, vernalization, morphogenetic responses, chlorophylogenesis, hemogenesis, rhizogenesis


The paper presents the findings on the impact of cold exposure at 4°C on chlorophylogenesis, hemogenesis and rhizogenesis in callus culture of winter wheat cultivars. The patterns of morphogenesis in the soft winter wheat in vitro culture during exposure to positive low temperature of various durations (15, 30 and 45 days) have been established. The callus culture of 2–3 passages of cv. Doridna, Statna and Astet was used. The results of experiments showed the preliminary vernalization of calluses for 30 and 45 days to strongly stimulate the chlorophylogenesis and hemogenesis, thereby increasing the frequency of obtaining the regenerant plants during further cultivation. The efficiency of these processes depended on the original cultivar genotype and cold treatment duration. The vernalization, associated with the VRN genes expression, manifested in morphogenesis change, was assumed to occur in the soft winter wheat in vitro culture.


Probl Cryobiol Cryomed 2022; 32(3): 214–225


Achrem M, Skuza L, Kalinka A, et al. Role of epigenetic mechanisms in plant response to low temperature. Acta Biol Crac Ser Bot. 2012; 54 (1): 7-15. CrossRef

Adamczuk A, Siegien I, Ciereszko I. Morphogenesis of plants in vitro under stress conditions. In: Łaska G. editor. Biological diversity - from cell to ecosystem. Białystok: Polish Botanical Society - Branch in Białystok; 2012. P. 25-40.

Atramentova LO, Utevska OM. [Statistical methods in biology]. Kharkiv: V.N. Karazin Kharkiv National University; 2007. 288 p. Ukrainian.

Avksentieva OO, Shulik VV. [Allelic state and effects of soft wheat VRN genes in vivo and in vitro]. Biosystems Diversity. 2016; 24(1): 222-30. Ukrainian. CrossRef

Avksentieva OO, Shulik VV. [Biotechnology of higher plants: in vitro culture]. Kharkiv: V.N. Karazin Kharkiv National University; 2017. 96 р. Ukrainian.

Avksentieva OO, Shulik VV, Taran NY. Research into the influence of contrasting trophic conditions of vernalization on the allelic state of VRN genes and the development rates of Triticum aestivum L. Biologija. 2018; 64 (1): 73-81. CrossRef

Benderradji L, Brini F, Kellou K. et al. Callus induction, proliferation, and plantlets regeneration of two bread wheat (Triticum aestivum L.) genotypes under saline and heat stress conditions. Int Sch Res. [Internet] 2011 15 Dec [Cited 11.09.2021]; 2012: 367851. Available from: CrossRef

Bilynska OV. Influence of spike pretreatment at low temperatures on efficiency of spring barley haploid production in anther culture in vitro. Probl Cryobiol Cryomed. 2020; 30 (1): 68-76. CrossRef

Delporte F, Pretova A, Jardin P, Watillon B. Morphohistology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat. Protoplasma. 2014; 251: 1455-70. CrossRef

Dennis ES, Peacock WJ. Vernalization in cereals. J Biol. 2009; 8(6): 57-67. CrossRef

Dubrovna OV, Morhun BV, Bavol AV. [Wheat biotechnology: selection and genetic engineering]. Kyiv: Lohos, 2014. 375 p. Ukrainian.

Fait VI. [Genetic control system for differences in the duration of vernalization in winter wheat]. Tsitol Genet. 2003; 37(5): 69-76. Russian. PubMed

Henderson IR, Shindo C, Dean C. The need for winter in the switch to flowering. Annu Rev Genet. 2003; 37: 371-92. CrossRef

Karimzadeh G, Francis D, Davies MS. Low temperature induced accumulation of protein is sustained both in root meristems and in callus in winter wheat but not in spring wheat. Ann Bot. 2000; 85: 769-77. CrossRef

Kondic-Sipka A, Hristov N, Kobiwski B. In vitro screening for low temperature tolerance of wheat genotypes. Genetika. 2006;38(2): 137-44. CrossRef

Morgun VV, Dubrovna OV, Morgun BV. [Obtaining stressresistant wheat plants by biotechnological methods]. In: [Plant physiology: achievements and new directions of development]. Kyiv: Lohos; 2017. p. 393-413. Ukrainian

Morgun VV, Kyryzyi DA, Shadchyna TK. [Ecophysiological and genetic aspects of adaptation of cultivated plants to global climate change]. Fiziologiya i biokhimiya kulturnykh rasteniy. 2010; 42(1): 3-23. Russian.

Muterko AF, Balashova IA, Fayt VI, Sivolap YuM. Molecular genetic mechanisms of regulation of growth habit in wheat. Cytol Genet. 2015; 49(1): 58-71. CrossRef

Parmar S, Sainger M, Chaudhary D, et al. Plant regeneration from embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars. Physiol Mol Biol Plants. 2012; 18: 177-83. CrossRef

Roesler EA, Manfroi E, Morás A, et al. Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes. Ciência Rural. [Internet] 2018 [cited 11.09. 2021]; 48(11): e20170806. Available from: CrossRef

Seldymyrova OA, Kruhlova NN, Zynatullyna AE. [The role of phytohormones in the induction of callusogenesis and the regulation of morphogenesis pathways of cereal calli in vitro: a review of the problem]. Nauchnyj rezultat. Ser. Fizioligiya. 2017; 3 (1): 8-13. Russian.

Shcherban' AB, Salina EA. [Epigenetic regulation of vernalization gene expression]. Tsitologiia. 2013; 55(4): 234-7. Russian. PubMed

Stelmakh AF, Fait VI, Martyniuk VR. [Genetic systems of the type and control of the rate of development of common wheat]. Tsitol Genet. 2000; 34(2): 39-45. Russian. PubMed

Zhuravlev YN, Omelko AM. Plant morphogenesis in vitro. Russ J Plant Physiol 2008; 55(5): 579-96. CrossRef




How to Cite

Avksentieva, O., Zhmurko, V., & Chumakova, V. (2022). Morphogenetic Responses of Triticum aestivum L. Calus Culture During Long-Term Effect of Low Positive Temperatures. Problems of Cryobiology and Cryomedicine, 32(3), 214–225.



Theoretical and Experimental Cryobiology