Effect of Incubation in NaCl Hypertonic Solutions on Changes of Adrenocorticocytes' Volume and Arrangement of Lipid Droplets

Authors

  • T. A. Yurchuk V.N.Karazin Kharkov National University, Kharkov
  • N. A. Chernobay Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

adrenocorticocytes, lipid droplets, Nile red, re-hydration, dehydration, steroid hormones

Abstract

Volumetric changes of cells and adrenocorticocytes’ (AC) intracellular space, within the range of which lipid droplets (LD) located, after the effect of NaCl hypertonic solutions were studied by the fluorescence and light microscopy. Under re-hydration after effect of 0.75 and 1M NaCl solutions the cell volume recovered in average up to 85% of the initial one during 20 min, and the volume of the space, occupied with LD increased up to 77 and 80%, correspondingly, i. e. the delayed recovery of space, occupied with LD and AC was observed. It is suggested that approaching of LD (depot of cholesterol) to the site of hormone synthesis due to volumetric changes of AC results in the increase of basal steroidogenesis rate after hypertonic effect.

Downloads

Published

2010-09-20

How to Cite

Yurchuk, T. A., Chernobay, N. A., Bozhok, G. A., Kovalenko, I. F., Bondarenko, T. P., & Rozanov, L. F. (2010). Effect of Incubation in NaCl Hypertonic Solutions on Changes of Adrenocorticocytes’ Volume and Arrangement of Lipid Droplets. Problems of Cryobiology and Cryomedicine, 20(3), 282–287. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/187

Issue

Vol. 20 No. 3 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. A. Yurchuk, N. A. Chernobay, G. A. Bozhok, I. F. Kovalenko, T. P. Bondarenko, L. F. Rozanov

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).