Seed viability of less spread introduced conifer plants after storage in liquid nitrogen

Authors

  • Emma R. Arapetyan Ivan Franko Lviv National University, Lviv, Ukraine https://orcid.org/0009-0007-9517-8192
  • Maryna O. Shcherbyna Ivan Franko Lviv National University, Lviv, Ukraine
  • Yurii M. Usatenko Ivan Franko Lviv National University, Lviv, Ukraine

Keywords:

seeds, liquid nitrogen, cryopreservation, viability, conifers, gene pool, Cupressaceae, Pinaceae

Abstract

Tolerance of seeds of plants from the family Cupressaceae (Cypress) Chamaecyparis lawsoniana (Lawson's cypress), Cryptomeria japonica (Japanese cypress), Platycladus orientalis (Oriental broadleaf) and Pinaceae (Pine) Larix kaempferi (Kaempfer's larch), Pseudotsuga menziesii (Menzies' pseudotsuga), Tsuga сanadensis (Canadian hemlock), which according to the International Classification are less spread plants, to storage conditions in liquid nitrogen were investigated for the first time. The results of scientific research into the preservation of seeds in liquid nitrogen, as a source of genetic information, confirmed that the investigated seeds of plants introduced in Western Ukraine retained their viability after storage. Soil germination of cryopreserved seeds increased compared to the control variant on 0.7% for C. japonica, 7.4% for L. kaempferi or decreased in T. canadensis by 2%. The growth and development of the control seedlings do not differ from seedlings obtained from cryopreserved seeds.

Probl Cryobiol Cryomed. 2026; 36(2): 122—127

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Published

2026-07-14

How to Cite

Arapetyan, E., Shcherbyna, M., & Usatenko, Y. (2026). Seed viability of less spread introduced conifer plants after storage in liquid nitrogen. Problems of Cryobiology and Cryomedicine, 36(2), 122—127. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/2195

Issue

Vol. 36 No. 2 (2026): Probl Cryobiol Cryomed

Section

Cryopreservation of Biological Resources

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