Conditions for Loach (Misgurnus fossilis) Embryo Vitrification in Cryoprotective Media

Authors

  • K. B. Mikson Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. F. Kopeika Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

embryos, mud loach, Misgurnus fossilis, cryoprotectant medium, vitrification

Abstract

Resistance of loach (Misgurnus fossilis) embryos to the effect of 5 cryoprotective media was investigated and the conditions of their vitrification were determined. The obtained experimental data demonstrated, that at a developmental stage, corresponding to the heart beating onset, the highest number of vitrified embryos (up to 100%) was observed during 30 min incubation within a cryoprotective medium, consisting of 30% sucrose, 10% ethylene glycol, 3% polyethylene oxide–1500 and 20% 1,2-propane diol. Vitrification temperature of loach embryos at this developmental stage was within –80...–176°C range.

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Published

2009-07-06

How to Cite

Mikson, K. B., Kopeika, E. F., & Linnik, T. P. (2009). Conditions for Loach (Misgurnus fossilis) Embryo Vitrification in Cryoprotective Media. Problems of Cryobiology and Cryomedicine, 19(2), 154–162. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/270

Issue

Vol. 19 No. 2 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 K. B. Mikson, E. F. Kopeika, T. P. Linnik

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