Peculiarities of Cryopreservation Effect on Functional Potential of Fetal Liver Hemopoietic Stem Cells of Various Gestation Terms

Authors

  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. G. Dubrava Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. V. Ostankova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. I. Grischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. Ye. Yampolskaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. A. Sirous Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

fetal liver cells, cryopreservation, hemopoietic precursors

Abstract

The capability of cryopreservation to modify structural and functional status of bioobject, including fetal liver cells (FLCs), may be applied for cell and tissue therapy optimisation. The efficiency of FLCs application is determined by the presence in them of a wide spectrum of cell populations, including hemopoietic stem cells. There has been established a change in phenotypic and functional characteristics of FLCs with gestation term prolongation, manifesting in a decrease in their colony-forming potential, CD34+ number and a simultaneous increase in Mac-1+ and LFA-1+ cells. Cryopreservation was shown as capable for a selective enrichment of 18 gestation day’s FLCs by hemopoietic precursors with a high proliferative potential. This fact may be determining one in their therapeutic effect manifestation.

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Published

2009-07-06

How to Cite

Goltsev, A. N., Dubrava, T. G., Ostankova, L. V., Grischenko, V. I., Yampolskaya, E. Y., Ostankov, M. V., Bondarovich, N. A., & Sirous, M. A. (2009). Peculiarities of Cryopreservation Effect on Functional Potential of Fetal Liver Hemopoietic Stem Cells of Various Gestation Terms. Problems of Cryobiology and Cryomedicine, 19(2), 186–199. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/273

Issue

Vol. 19 No. 2 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. N. Goltsev, T. G. Dubrava, L. V. Ostankova, V. I. Grischenko, E. Ye. Yampolskaya, M. V. Ostankov, N. A. Bondarovich, M. A. Sirous

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