Protective Effect of Methylcellulose during Cryopreservation of Inoculated Cells in Serum-Free Medium
Keywords:inoculated cells, cryopreservation, preservation medium, methylcellulose
There was experimentally shown a possibility to replace blood serum for methylcellulose (MC) in the media for cryopreservation of inoculated cells. Combination of 0.1% MC with 5% dimethyl sufoxide (DMSO) in the preservation media provided the highest indices of post-thaw cell survival. Freeze-thawing of cells in the medium contained MC, but without DMSO resulted in a significant reduction of survived cells. The cells, which were pre-cultured in the medium with 0.5% fetal serum (FS) and 0.1% MC, showed higher survival post freeze-thawing in serum-free medium comparing to the cells, which we pre-cultured in the medium with 10% FS. Probl Cryobiol Cryomed 2013; 23(2):116â€“123.
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Copyright (c) 2020 Svetlana V. Koschiy, Anatoliy N. Goltsev, Igor P. Vysekantsev, Valentina F. Martsenyuk, Tatyana M. Gurina, Larisa V. Sokol
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