EPR Study of Freezing Effect on Structural-Functional State of Mitochondrial Membrane
Keywords:freeze-thawing, mitochondria, electron paramagnetic resonance, spin probe reduction, structure reorganization
Structure and electron transport function of rat liver mitochondria membranes have been studied by EPR method of spin probes. It is established that at single rapid freezing of organelles down to â€“196Â°C there is an increase in the looseness of membranes of mitochondria without its structural changes, affecting the condition of proteins of electron transfer chain. Two-fold rapid freezing of suspension of mitochondria results in significant damage of membrane structural organization, inducing the disappearing of thermotropic structural transition in the range of 10Â°C.
How to Cite
Copyright (c) 2020 O. A. Nardid
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).