Effect of Equine Erythrocyte Incubation at 49°C on Amphiphilic Substance Efficiency Under Hypertonic Stress Conditions

Authors

  • N. M. Shpakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Pisarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. V. Orlova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

equine erythrocytes, hypertonic hemolysis, spectrin, amphiphilic compounds

Abstract

The effect of amphiphilic compounds (C10, DM, TFP) and heat preliminary treatment of equine erythrocytes (49°C) on cell sensitivity to hypertonic stress (4.0 M NaCl) has been investigated. All amphiphilic compounds were shown as reducing the level of hypertonic hemolysis in equine erythrocytes. Erythrocyte incubation at 49°C, resulting in spectrin denaturation, reduces the level of erythrocyte hypertonic damage at 37°C and increases it at 0°C. A decrease in the efficiency of all studied amphiphilic compounds is observed under the mentioned conditions.

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Published

2009-05-25

How to Cite

Shpakova, N. M., Pisarenko, N. A., & Orlova, N. V. (2009). Effect of Equine Erythrocyte Incubation at 49°C on Amphiphilic Substance Efficiency Under Hypertonic Stress Conditions. Problems of Cryobiology and Cryomedicine, 19(1), 49–55. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/286

Issue

Vol. 19 No. 1 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. M. Shpakova, N. A. Pisarenko, N. V. Orlova

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