Cryopreservation of Insect Germplasm

Authors

  • Liana I. Relina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Aleksandr K. Gulevsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, insects, reproductive products

Abstract

The review describes the basic problems of cryopreservation of insect germplasm as well as approaches to their solution, which have been developed to date. The peculiarities of cryopreservation procedures for insect embryos and sperm are discussed in comparison with cryopreservation methods for reproductive products of other species. A brief description of species, for which the cryopreservation protocols are considered, substantiates the need for long-term storage of their genetic material. In the review we categorized the insect species, for which the designed low-temperature storage techniques are presented, in systematic taxa (orders). The prospects for the further development in this area are discussed. Probl Cryobiol Cryomed 2013; 23(3):205–227.

Author Biographies

Liana I. Relina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Biochemistry of Cold Adaptation

Aleksandr K. Gulevsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Biochemistry of Cold Adaptation

References

Anderson K.V., Lengyel J.A. Changing rates of DNA and RNA synthesis in Drosophila embryos. Dev. Biol. – 1981; 82 (1): 127–138.

AQUAGAMETE COST European Cooperation in Science and Technology Fish reproductive biology and biotechnology Fish Physiology and Genomics laboratory Institut National de la Recherche Agronomique (INRA). [web-site] http://aquagamete.webs.upv.es/france/ (accessed on 24.04 2013).

Cantwell G.E. (appi A.J. Stoffolano J.G. Embryonic and postembryonic development of the house fly (Musca domestica L.) Agriculture Research Service, United States Department of Agriculture, Washington, DC. – 1976.

Collins A.M., Mazur P. Chill sensitivity of honey bee, Apis melli-fera, embryos. Cryobiology. – 2006; 53 (1): 22–27.

Cosi E., Abidala M.T., Roversi P.F. The effect of Tween80 on eggshell permeabilization in Galleria mellonella (L.) (Lepidoptera, Pyralidae). Cryo-Letters. – 2010; 31 (4): 291–300.

Davisson M. FIMRe: Federation of International Mouse Resources: global networking of resource centers. Mamm. genome 2006; 17 (5): 363–364.

Gulevsky A.K., Relina L.I. Molecular and genetic aspects of protein cold denaturation. Cryo-Letters 2013; 34 (1): 62–82.

Hatakeyama M., Sumitani M. Preservation of a transgenic strain of the sawfly, Athalia rosae (Hymenoptera) by artificial fertilization using cryopreserved sperm. Insect Mol Biol; 14 (1): 105–109.

Harbo J.R. Survival of honey bee (Hymenoptera: Apidae) spermatozoa after two years in liquid nitrogen (–196°C). Ann Entomol Soc Am 1983; 76 (5): 890–891.

Holler T.C., Davidson J.L., Suarez A., Garsia R. Release of sterile Mexican fruit flies for control of feral populations in the Rio Grande Valley of Texas and Mexico. J. Rio Grande Valley Hort. Soc. – 1984;37 – P. 113–121.

Hopkins B.K., Herr C. Factors affecting the successful cryopreservation of honey bee (Apis mellifera) spermatozoa. Apidologie 2010; 41 (5): 548–556.

Houle D., Kondrashov A.S., Yampolsky L.Y. et al. The effect of cryopreservation on the lethal mutation rate in Drosophila melanogaster. Genet Res 1997; 69 (3): 209–213.

Kaftanoglu O., Peng Y.S. Preservation of honeybee sper-matozoa in liquid nitrogen. J Apicult Res 2012; 23 (3): 157–163.

Karja N.M., Otoi T., Wongstrikeao P. et al. In vitro development and post–thaw survival of blastocysts derived from delipidated zygotes from domestic cats. Theriogenology 2006;65 (2): 415–423.

Kusuda J. Noguchi T., Onimaru K., Yamashita O. Maturation and hatching of eggs from silkworm ovaries preserved in liquid nitrogen. J Insect Physiol 1985; 31 (12): 963–967.

Leopold R.A. Cold storage of insects: using cryopreservation and dormancy as an aid to mass rearing. Area-wide control of insect pests integrating the sterile insect and related nuclear and other techniques. FAO/IAEA International Conference, Penang, Malaysia. 1998: 235–267.

Leopold R.A., Atkinson P.W. Cryopreservation of sheep blow fly embryos, Lucilia cuprina (Diptera: Calliphoridae). Cryo-Letters. – 1999; 20 (1): 37–44.

Leopold R.A., Rinehart J.P. A template for insect cryopreservation. In: Low Temperature Biology of Insect / Ed. by D.L. Denlinger, R.E. Lee. – Cambridge: University Press, 2010: 325–341.

Leopold R.A., Wang W.B., Berkebile D.R., Freeman T.P. Cryopreservation of embryos of the New World screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae). Ann Entomol Soc Amer 2001; 94 (5): 695–701.

Limbourg B., Zalokar M. Permeabilization of Drosophila eggs. Dev Biol 1973; 35 (2): 382–377.

Liu X.H., Mazur P. Effects of sugars on the kinetics of drying and on the survival of partially dehydrated larvae of Anopheles mosquitoes. J Insect Physiol 2003; 49 (7): 685–695.

Liu X.H., Zhang T., Rawson T.M. Effect of cooling rate and partial removing of yolk on the chilling injury in zebrafish (Danio rerio) embryos. Theriogenology 2001; 55 (8): 1719–1731.

Luo L., Pang Y., Chen Q., Li G. Cryopreservation of the late stage embryos of Spodoptera exigua (Lepidoptera: Noctuidae). Cryo-Letters 2006; 27 (6): 341–352.

Lynch D.V., Lin T.T., Myers S.P. et al. A two-step method for permeabilization of Drosophila eggs. Cryobiology 1989; 26 (5): 445–452.

Mazur P. Equilibrium, quasi-equilibrium, and nonequilibrium freezing of mammalian embryos. Cell Biophys 1990; 17 (1): 53–92.

Mazur P., Cole K.W., Hall J.W. et al. Cryobiological preservation of Drosophila embryos. Science 1992; 258 (5090): 1932–1935.

Mazur P, Cole K.W., Mahowald A.P. Critical factors affecting the premeabilization of Drosophila embryos by alkanes. Cryobiology 1992; 29 (2): 210–239.

Mazur P., Cole K.W., Schreuders P.D., Mahowald A.P. Contributions of cooling and warming rate and developmental stage to the survival of Drosophila embryos cooled to –205°C. Cryobiology 1993; 30 (1): 45–73.

Mazur P., Leibo S.P., Seidel G.E.Jr. Cryopreservation of the germplasm of animals used in biological and medical research: Importance, impact, status and future directions. Biol Reprod 2008; 78 (1): 1–12.

Mazur P., Schneider U., Mahowald A.P. Characteristics and kinetics of subzero chilling injury in Drosophila embryos. Cryobiology 1992; 29 (1): 39–68.

McGrath J.J. Cold shock: thermoelastic stress in chilled bio-logical membranes. Network thermodynamics, heat and mass transfer in biotechnology / Ed. by K.R. Diller. – New York: American Society of Mechanical Engineers, 1987: 57–66.

Mochida Y., Takemura Y., Kanda T., Horie Y. Fertilized eggs obtained from transplantation of frozen ovaries and par-thenogenesis in combination with artificial insemination of frozen semen of the silkworm, Bombyx mori. Cryobiology 2003; 46 (2): 153–160.

Mochida Y., Takemura Y., Ohnuma A. et al. Fertilization of eggs developed from the cryopreserved and transplanted ovaries by artificial insemination with cryopreserved semen in the silkworm, Bombyx mori. J Insect Biotechnol Sericol 2007; 76 (2): 97–100.

Myers S.P., Lynch D.V., Knipple D.C. et al. Low-temperature sensitivity of Drosophila melanogaster embryos. Cryobiology 1988;25 (6): 544–545.

Myers S.P., Pitt R.E., Lynch D.V., Steponkus P.L. Charac-terization of intracellular ice formation in Drosophila melanogaster embryos. Cryobiology 1989; 26 (5): 472–484.

Myers S.P., Steponkus P.L. Subzero chilling sensitivity of Drosophila melanogaster embryos. Cryobiology 1990; 27 (6): 651–652.

National Project Peproductive Cryobank (Omaha's Henry Doorly Zoo & Aquarium). [web-site] http://www.omahazoo.com/conservation/reproductive-sciences/national-projects/reproductive-cryobank (accessed on 24.04 2013).

Nunamaker R.A., Lockwood J.A. Cryopreservation of embryos of Culicoides sonorensis (Diptera: Ceratopogonidae). J Med Entomol 2001; 38 (1): 55–58.

Oishi K., Sawa M., Hatakeyama M., Kageyama Y. Genetics and biology of the sawfly, Athalia rosae (Hymenoptera). Genetica 1993; 88 (2–3): 119–127.

Papassideri I., Margaritis L.H., Gulik-Krzywicki T. The eggshell of Drosophila melanogaster VI. Structural analysis of the wax layer in laid eggs. Tissue Cell 1991; 23 (4): 567–575.

Rall W.F., Meyer T.K. Zona fracture damage and its avoidance during the cryopreservation of mammalian embryos. Theriogenology 1989; 31 (3): 683–692.

Rajamohan A., Leopold R.A. Cryopreservation of Mexican fruit flies by vitrification stage selection and avoidance of thermal stress. Cryobiology 2007; 54 (1): 44–54.

Rajamohan A., Leopold R.A., Wang W.B. et al. Cryopreservation of Mediterranean fruit fly embryos. Cryo-Letters 2003; 24 (2): 125–132.

RDA-Genebank Information Center, Republic of Korea (Silk-worm breeding lab., Sericulture & Apiculture Division, Department of Sericulture and Entomology). [web-site] http://www.genebank.go.kr/eng/silkworm/introduction.jsp (accessed on 24.04 2013).

Research Project: Development of A Robotic System for High Through-Put Insect Embryo Cryopreservation (United States Department of Agriculture, Agricultural Research Service; Location: Insect Genetics and Biochemistry Research, Project Number: 5442-21220-027-04). [web-site] http://www.ars.usda.gov/research/projects/projects.htm?accn_no=421831 (accessed on 09.11 2012).

Research Project: Development of Cold Storage Technology for Mass-Reared and Laboratory-Colonized Insects (United States Department of Agriculture, Agricultural Research Service; Location: Insect Genetics and Biochemistry Research, Project Number: 5442-22000-040-00) [web-site] http://www.ars.usda.gov/research/projects/projects.htm?accn_no=409603 (accessed on 09.11 2012).

Research Project: Evaluation of a Mass-Cryopreservation System for Insect Embryos (United States Department of Agriculture, Agricultural Research Service; Location: Insect Genetics and Biochemistry Research, Project Number: 5442-21220-027-02). [web-site] http://www.abadrl.ars.usda.gov/research/projects/projects.htm? ACCN_NO=414373] (accessed on 09.11 2012).

Research Project: Insect Cryopreservation, Dormancy, Genetics and Biochemistry (United States Department of Agriculture, Agricultural Research Service; Location: Insect Genetics and Biochemistry Research Project Number: 5442-21220-027-00). [web-site] http://www.larrl.ars.usda.gov/research/projects/projects.htm? ACCN_NO=421077 (accessed on 09.11 2012).

Roversi P.F., Cosi E., Irdani T. Chill sensitivity and cryopreser-vation of eggs of the great wax moth Galleria mellonella (Lepidoptera, Pyralidae). Cryobiology 2008; 56 (1): 1–7.

Schierenberg E. Developmental strategies during early embryo-genesis of Caenorhabditis elegans. J Embryol Exp Morphol 1986; 97: 31–44.

Shinbo H. Survival of larval ovaries and testes frozen in liquid nitrogen in the silkworm, Bombyx mori. Cryobiology 1989; 26 (4): 389–396.

Sonnenblick B.P. The early embryology of Drosophila melanogaster. In: Biology of Drosophila / Ed. by M. Demerec. New York: Hafner, Wiley and Son, 1965: 62–167.

Spradbery J.P. A manual for the diagnosis of screwworm fly. Canberra: CSIRO Division of Entomology. – 1991.

Steponkus P.L., Caldwell S. An optimized procedure for the cryopreservation of Drosophila melanogaster embryos. Cryo-Letters 1993;14 (6): 375–380.

Steponkus P.L., Myers S.P., Lynch D.V. et al. Cryopreservation of Drosophila melanogaster embryos. Nature 1990; 345 (6271): 170–172.

Stucky M., Hopkins B.K., Herr C. Cryopreservation of Honey Bee Spermatozoa. In: The 34th Annual IETS conference. Denver, Colorado, 2008: 94.

Sulston J.E., Schierenberg E., White J.G., Thomson J.N. The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. – 1983 – Vol.100 (1): 64–119.

Takemura Y., Kanda T., Horie Y. Artificial insemination using cryopreserved sperm in the silkworm, Bombyx mori. J Insect Physiol 2000; 46 (4): 491–497.

Takemura Y., Sahara K., Mochida Y., Ohnuma A. Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori. J Insect Physiol 2006; 52 (10): 1021–1026.

Tamura T., Sakate S. Preservation of spermatozoa of the silk-worm, Bombyx mori, by freezing. Acta Sericologica 1985; (13): 123–128.

Taylor M.A., Guzman-Novoa E., Morfin N., Buhr M.M. Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratios. Theriogenology 2009; 72 (2): 149–159.

Umeya Y. Morphological study on ovarian transplantation in the silkworm, Bombyx mori. Zool Magaz Japan 1933; 45 (2): 59–66.

Ushijima H., Yamakawa H. Nagashima H. Cryopreservation of bovine pre-morula stage in vitro matured/in vitro fertilized embryos after delipidation and before use in nucleus transfer. Biol Reprod 1999; 60 (2): 534–539.

Valencia M.D., Miller L.H., Mazur P. Permeability of intact and dechorionated eggs of the Anopheles mosquito to water vapor and liquid water: a comparison with Drosophila. Cryobiology 1996; 33 (1): 142–148.

Valencia M.D., Miller L.H., Mazur P. Permeabilization of eggs of the malaria mosquito Anopheles gambiae. Cryobiology 1996; 33 (1): 149–162.

Wang W.B., Leopold R.A., Freeman T.P. Cryopreservation of Dipteran embryos. Cryobiology 2000; 41 (4): 349–350.

Wang W.B., Leopold R.A. (elson D.R., Freeman T.P. Cryopreservation of Musca domestica (Diptera: Muscidae) embryos. Cryobiology 2000; 41 (2): 153–166.

Wyss J.H. Screwworm eradication in the Americas. Ann NY Acad Sci 2000; 916: 186–193.

Yocum G.D., Rinehart J.P., West M., Kemp W.P. Interrupted incubation and short-term storage of the alfalfa pollinator Megachile rotundata (Hymenoptera: Megachilidae): a potential tool for synchronizing bees with bloom. J Econ Entomol 2010;103 (2): 234–241.

Downloads

Published

2013-09-30

How to Cite

Relina, L. I., & Gulevsky, A. K. (2013). Cryopreservation of Insect Germplasm. Problems of Cryobiology and Cryomedicine, 23(3), 205–227. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/298