Effect of Oxyethylated Glycerol with Polymerization Degree n = 5 on Human Hemoglobin Thermostability

Authors

  • Aleksandra V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yulianna S. Govorova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Antonina M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

melting, hemoglobin, thermal stability, oxyethylated derivative of glycerol with polymerization degree n = 5, differential adiabatic scanning microcalorimetry

Abstract

The effect of oxyethylated derivative of glycerol with polymerization degree n = 5 (OEGn=5) on thermodynamic and kinetic parameters of hemoglobin melting was studied by the method of differential adiabatic scanning microcalorimetry. It was shown that increase of OEGn=5 concentration in solution resulted in reduction of the temperature and calorimetric enthalpy of hemoglobin thermodenaturation. It was suggested that the increasing of cryoprotective concentration induced the loosening of hemoglobin molecules thereby contributing to the reduction of thermal stability of protein. The possibility of using kinetic approach when analyzing hemoglobin melting process was shown. Probl Cryobiol Cryomed 2013; 23(2):135–142.

Author Biographies

Aleksandra V. Zinchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Yulianna S. Govorova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Antonina M. Kompaniets, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryoprotectants

References

Kovrigin E.L., Potekhin S.A. Microcalorimetric study of dimethyl sulfoxide effect on thermal denaturation of lysozyme. Biofizika 1996; 41 (6): 1201–1206.

Lapshina E.A., Zavodnik I.B., Ignatenko V.A., Stepuro I.I. Thermal stability and functional properties of human hemoglobin in the presence of aliphatic alcohols. Molekulyarnaya Biologiya 1992; 26 (2): 315–320.

Lyubarev A.E., Kurganov B.I. Investigation of irreversible thermal denaturation of proteins by differential scanning calorimetry. Uspekhi Biologicheskoy Khimii 2000; 40: 43–84.

Solovyeva A.S. Calorimetric studies of thermal denaturation of isolated and membrane-bound proteins with some cryoprotectants prior to and after cooling down to –196°C [Author's abstract of thesis of the candidate of biological sciences]. – Kharkov, 1999.

Shrago M.I., Guchok M.M., Kalugin Yu.V. Some principles of directed synthesis of cryoprotectants. In: Aktualnye problemy kriobiologii. Ed. by N.S. Pushkar, A.M. Belous. Kiev: Naukova Dumka, 1981: 157–201.

Arosio D., Kwansa H.E., Gering H. et al. Statistic and dynamic light scattering approach to the hydration of hemoglobin in the presence of osmolites. Biopolymers 2002. – 63 (1): 1–11.

Meng F.-G., Hong Y.-K., He H.-W. et al. Osmophobic Effect of Glycerol on Irreversible Thermal Denaturation of Rabbit Creatine Kinase. Biophys J 2004; 87 (4): 2247–2254.

Michnik A., Drzazga Z., Kluczewska A., Michalik K. Differential scanning microcalorimetry study of the thermal denaturation of haemoglobin. Biophys Chem 2005; 118 (2–3): 93–101.

Sanchez-Ruiz J.M. Theoretical analysis of Lumry-Eyring models in differential scanning calorimetry. Biochem J 1992; 64 (4): 921–935.

Sengupta B., Swenson J. Properties of normal and glycated human hemoglobin in presence and absence of antioxidant. Biochem Biophys Res Commun 2005; 334 (3): 954–959.

Zhivotova E.N., Zinchenko A.V., Kuleshova L.G. et al. Low-temperature phase behaviour of the binary system water-oxyethylated glycerol of polymerization degree n = 5 and intermolecular interactions in the system. Mol Phys 2008; 106 (14): 1751–1759.

Published

2013-06-20

How to Cite

Zinchenko, A. V., Govorova, Y. S., & Kompaniets, A. M. (2013). Effect of Oxyethylated Glycerol with Polymerization Degree n = 5 on Human Hemoglobin Thermostability. Problems of Cryobiology and Cryomedicine, 22(2), 135-142. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/30

Issue

Section

Theoretical and Experimental Cryobiology