Clinical Case of Using Cryopreserved Spermatozoa Obtained by Biopsy in Patients with Azoospermia

Authors

  • Viktoriya V. Podufaliy Department of Cryobiology of Reproduction System, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov Third Municipal Clinical Hospital, Lviv

Keywords:

spermatozoa, cryopreservation, assisted reproductive technologies

Abstract

Previously developed highly effective method for cryopreservation of normozoospermic ejaculate was applied for single epididymal spermatozoa for following using in assisted reproductive technologies (ART). The cells were cooled in microcapillaries with solution of 20% sucrose and 15% glycerol from 22 down to –35°C with rate of 1 deg/min, from –35 down to –70°C with rate of 20 deg/min and thereafter plunged into liquid nitrogen. Post-thaw survival of spermatozoa was 92%. Oocytes were fertilized by injection of single spermatozoa, the embryos were cultured, assessed for pronuclei peresence and transfered into uterus. Pregnancy rate after using cryopreserved cells was higher (53.1%) than after using fresh asperated cells (46.1%). Thus the utilized method of cryopreservation could be effectively used in ART for patients with azoospermy. Probl Cryobiol Cryomed 2013; 23(3):260–266.

References

Treatment of female and male infertility. Assisted reproductive technologies. Ed. by Kulakov V.I., Leonov B.V., Kuzmichev L.N. Moscow: Med. Inform. Agenstvo, 2005.

Khilkevich L.V., Zdanovsky V.M., Togobetsky S., Gogolevsky P.A. Additional reproductive technologies (PESA and MESA) at infertility treatment stipulated by male factor. Problemy Reproduktsii. – 1998; 2: 29–33.

Yurchenko G.G., Dunaevskaya A.V., Kramar M.I. Effect of derivation method of motile fractions of gametes from human ejaculate on their morphofunctional integrity after cryopreservation. Problems of Cryobiology. – 2001; (2): 30–35.

Craft I., Bennet V., Nicholson N. Fertilising ability of testicular spermatozoa. Lancet.– 1993; 2 (342).– C. 864.

Craft I., Tsirigotis M. Simplified recovery, preparation and cryopreservation of testicular spermatozoa. J Hum Reprod 1995; 10 (7): 1623–1627.

Desai N., AbdelHafez F., Sabanegh E. Paternal effect on genomic activation, clinical pregnancy and live birth rate after ICSI with cryopreserved epididymal versus testicular spermatozoa. Reprod Biol Endocrinol 2009; 3 (7): 142–149.

Devroey P., Liu J., Nagy Z. et al. Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection (TESE and ICSI). Fertil Steril 1994; 62 (3): 639–641.

Devroey P., Nagy Z., Tournaye H. et al. Outcome of itracytoplasmic sperm injection with testicular spermatozoa in obstructive and non-obstructive azoospermia. J Hum Reprod 1996; 11 (5): 1015–1018.

Gardner D., Lane. M., Stevens J. Schoolcraft W. Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential. Fertil Steril 2001; 76 (6): 1175–1180.

Hovatta O., Moilanen J., von Smitten K. et al. Testicular needle biopsy, open biopsy, epididymal aspiration and intracytoplasmic sperm injection in obstructive azoospermia. J Hum Reprod 1995; 10 (10): 2595–2599.

Jacques I., Phillips S., Hemmingset R. et al. Ongoing pregnancy after ICSI of frozen-thawed PESA-retrieved spermatozoa and IVF in a controlled natural cycle. Reprod BioMed Online 2005; 10 (5): 650–652.

Janzen N. Use of electively cryopreserved microsurgically aspirated epididymal sperm with IVF and intracytoplasmic sperm injection for obstructive azoospermia. Fertil Steril 2000; 74 (4): 696–701.

Jin L., Jiang L.Y., Zhu G.J. et al.Comparison between the results of ICSI with fresh and with frozen-thawed sperm obtained by PESA to treat azoospermia. Zhonghua Nan Ke Xue 2006; 12 (5): 443–449.

Kalsi J., Thum MY., Muneer A. Analysis of the outcome of intracytoplasmic sperm injection using fresh or frozen sperm. Br J Urol 2011; 107 (7): 1124–1128.

Munne S., Alikani M., Tomkin G. et al. Embryo quality and chromosome abnormalities after ICSI. Abstract from the IXth World Congress on IVF and Alternate Assisted Reproduction. J Assist Reprod Genet 1995; 76 (12): 76.

Ou L., Guo YH., Sun YP. et al. Outcomes of ICSI with micro-amount frozen-thawed sperm obtained by PESA or TESA in the treatment of azoospermia. Zhonghua Nan Ke Xue 2010; 16 (4): 328–332.

Patrizio P. Cryopreservation of epididymal sperm. Mol Cell Endocrinol 2000; 169 (1–2): 11–14.

Schoysman R., Vanderzwalmen P., Nus M. Pregnancy after fertilization with human testicular spermatozoa. Lancet 1993; 13 (342): 1237.

Silber S., Nagy Z., Liu J. et al. The use of epididymal and testicular spermatozoa for intracytoplasmic sperm injection: the genetic implications for male infertility. J Hum Reprod 1995; 10 (8): 2031–2043.

Silber S., Van Steirteghem A.C., Liu J. et al. High fertilization and pregnancy rates after sperm obtained from testicle biopsy. J Hum Reprod 1995; 10 (1): 148–152.

Tournaye H., Merdad T., Silber S. et al. No differences in out-come after intracytoplasmic sperm injection with fresh or with frozen-thawed epididymal spermatozoa. J Hum Reprod 1999; 14 (1): 90–95.

Westlander G., Hamberger L., Hanson Ch. et al. Diagnostic epididymal and testicular sperm recovery and genetic aspects in azoospermic men. J Hum Reprod 1999; 14 (1): 118–122.

World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge, UK, 1999.

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Published

2013-09-30

How to Cite

Podufaliy, V. V. (2013). Clinical Case of Using Cryopreserved Spermatozoa Obtained by Biopsy in Patients with Azoospermia. Problems of Cryobiology and Cryomedicine, 23(3), 260–266. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/302

Issue

Vol. 23 No. 3 (2013): Problems of Cryobiology and Cryomedicine (Problemy Kriobiologii i Kriomediciny) (formerly Problems of Cryobiology)

Section

Cryomedicine, Clinical and Experimental Transplantology

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Copyright (c) 2020 Viktoriya V. Podufaliy

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