Developing of Cryopreservation Method of Marek’s Disease Virus

Authors

  • M. Yu. Stegniy Institute of Experimental and Clinical Veterinary Medicine, Kharkov
  • B. T. Stegniy Institute of Experimental and Clinical Veterinary Medicine, Kharkov
  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, Marek’s disease, virus, vaccine

Abstract

The cryopreservation method, consisting in application of two-stage freezing regimen down to -30°C with 1°C/min rate, from –30 down to –70°C with 5–10°C/min rate with following plunging into liquid nitrogen (–196°C) at that composition of protective media 199 nutrient media 80% with adding of inactivated serum of cattle blood 10% and dimethyl sulfoxide 10% has been developed for Marek’s disease virus.

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Published

2013-12-22

How to Cite

Stegniy, M. Y., Stegniy, B. T., & Goltsev, A. N. (2013). Developing of Cryopreservation Method of Marek’s Disease Virus. Problems of Cryobiology and Cryomedicine, 18(4), 417–419. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/342

Issue

Vol. 18 No. 4 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 M. Yu. Stegniy, B. T. Stegniy, A. N. Goltsev

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