Towards a Medically Approved Technology for Large-Scale Stem Cell Banks: Tools and Methods

Authors

  • A. Katsen-Globa Fraunhofer IBMT, St. Ingbert
  • J. C. Schulz Fraunhofer IBMT, St. Ingbert
  • J. S. Baunach Fraunhofer IBMT, St. Ingbert
  • F. Ehrhart Fraunhofer IBMT, St. Ingbert
  • Y.-J. Oh Fraunhofer IBMT, St. Ingbert
  • U. Schön Fraunhofer IBMT, St. Ingbert
  • O. Kofanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Scienses of Ukraine, Kharkov
  • A.F. J. Beier Fraunhofer IBMT, St. Ingbert
  • S. Wiedemeier Institute for Bioprocessing and Analytical Measurement Techniques, Heilbad Heiligenstadt
  • J. Metze Institute for Bioprocessing and Analytical Measurement Techniques, Heilbad Heiligenstadt
  • S. Shirley Fraunhofer IBMT, St. Ingbert
  • D. Spitkovsky University of Cologne
  • A. Sachinidis University of Cologne
  • J. Hescheler University of Cologne
  • H. Zimmermann Fraunhofer IBMT, St. Ingbert

Keywords:

embryonic stem cells, cryopreservation, , stem cell banking, cryomicroscopy, cryotechnique, scanning electron microscopy

Abstract

The importance, of the development of stem cell cryobanking has increased recently with an augmentation of stem cell research and its therapeutic applications. The development of therapies is, among other things, limited by high sensitivity of stem cells to freezingthawing procedures. Thus, new approaches are needed for preservation and related evaluation methods, as well as new technologies for long term storage of large numbers of stem cells. Here we present selected recent improvements of stem cell cryopreservation, e.g. for freezing of adherent human embryonic stem cells using gel-like matrices. We report the application and performance of novel microsystem-based cryosubstrates and devices and describe new evaluation methods and the results of a thermal stress cycle study.

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Published

2008-12-22

How to Cite

Katsen-Globa, A., Schulz, J. C., Baunach, J. S., Ehrhart, F., Oh, Y.-J., Schön, U., Kofanova, O., Beier, A. J., Wiedemeier, S., Metze, J., Shirley, S., Spitkovsky, D., Sachinidis, A., Hescheler, J., & Zimmermann, H. (2008). Towards a Medically Approved Technology for Large-Scale Stem Cell Banks: Tools and Methods. Problems of Cryobiology and Cryomedicine, 18(4), 468–478. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/355

Issue

Vol. 18 No. 4 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 A. Katsen-Globa, J. C. Schulz, J. S. Baunach, F. Ehrhart, Y.-J. Oh, U. Schön, O. Kofanova, A.F. J. Beier, S. Wiedemeier, J. Metze, S. Shirley, D. Spitkovsky, A. Sachinidis, J. Hescheler, H. Zimmermann

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