Peripheral Blood Erythrocytes at Various Types of Hypothermia of Homoiothermal Organism
Keywords:craniocerebral hypothermia, general hypothermia, immersion hypothermia, erythrocytes, erythrocytes forms, osmotic fragility, hemolysis
The research deals with effect of hypothermia of homoiothermal organism on populations, osmotic fragility and hemolysis rate in erythrocytes of peripheric blood of rats. The hypothermic state was initiated by different methods: craniocerebral hypothermia (down to rectal temperature (RT) of 32.5 ±0.5°C) on a background of blockade of thermoregulation centres (TC) using special device for programmed cooling, whole body immersion hypothermia (WBIH) (RT of 27.5 ±0.5°C) using Porsolt’s modified test (swimming in ice cold water) and whole body hypothermia by method of closed vessel according Anjus-Bakhmet’ev-Giaja (CVWBH) on a background of TC blockade resulted from increasing level of hypoxia-hypercapnia (RT of 16.5 ±0.5°C). Analysis of obtained results showed that independently of depth and the method of initiating hypothermia of homoiothermal organism we observed reversible changes differing by expression rate: the discocytes number decreased, the share of changed shapes increased (stomatocytes and spherocytes); as well as the rise in osmotic fragility and hemolysis level of erythrocytes. In 24 hrs post hypothermia we observed the tendency for rising discocyte content on account of flattened forms, an increase of proportion of changed erythrocyte forms (stomatocytes and spherocytes), the osmotic fragility of erythrocytes turned back to the control level, and after CVWBH it decreased even more, the tolerance of cells to hemolysis factors rised, especially after WBIH.
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