Temperature Ranges of Phase Transformations in the Cryoprotective Media Components Determined by Thermoplastic Deformation Method
Keywords:thermoplastic deformation, fluidity limit, recrystallization, eutectic crystallization (melting), cryoprotectant
Phase transformations in multicomponent cryoprotective media and their components were investigated during freeze-thawing using the method of thermoplastic deformation. The research objects included solution of cryoprotectant DMSO (10%) and the components of cryoprotective media being often utilized as a solvents for biologicalsâ€™ cryoprotective media (distilled water, physiological saline and the culture media: medium 199, Hanksâ€™ solution, DMEM). The paper represents the temperature ranges of crystallization (melting) of eutectic concentration mixture for DMSO solution (from â€“87 to â€“67Â°C), eutectic crystallization (melting) of physiological solution and the culture media (from â€“37 to â€“21Â°C), recrystallization before melting of bulk ice (from â€“21 to â€“15Â°C) and following melting of bulk ice (from â€“15 to â€“4.5Â°C).
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Copyright (c) 2020 T. M. Gurina, A. L. Kirilyuk
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