Effect of Cryopreservation and Storage under Mineral Oil on Pheno- and Genotype Features of Escherichia coli Clinical Strains Isolated from Different Biotopes
Keywords:Escherichia coli, biotope, antibiotic sensitivity, plasmids, cryopreservation
The differences between the spectra of sensitivity to antibiotics of Escherichia coli strains isolated from different biotopes were found. It was found that storage at â€“196Â°Ð¡ during 5 years (observation term) of bacteria frozen under protection of 10% dimethyl sulfoxide solution did not change the spectrum of antibiotic resistance. After storage under the layer of mineral oil at the temperature of 6...8 Â°Ð¡ and subculturing some strains lost their resistance to beta-lactams, that was probably due to the loss of specific plasmids.Â
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Copyright (c) 2020 Yu. V. Voyda, S. V. Biryukova, I. P. Vysekantsev
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