Effect of Cryopreservation and Storage under Mineral Oil on Pheno- and Genotype Features of Escherichia coli Clinical Strains Isolated from Different Biotopes

Authors

  • Yu. V. Voyda Kharkiv Medical Academy of Postgraduate Education of the Ministry of Health Care of Ukraine, Kharkov
  • S. V. Biryukova Kharkiv Medical Academy of Postgraduate Education of the Ministry of Health Care of Ukraine, Kharkov
  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

Escherichia coli, biotope, antibiotic sensitivity, plasmids, cryopreservation

Abstract

The differences between the spectra of sensitivity to antibiotics of Escherichia coli strains isolated from different biotopes were found. It was found that storage at –196°С during 5 years (observation term) of bacteria frozen under protection of 10% dimethyl sulfoxide solution did not change the spectrum of antibiotic resistance. After storage under the layer of mineral oil at the temperature of 6...8 °С and subculturing some strains lost their resistance to beta-lactams, that was probably due to the loss of specific plasmids. 

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Published

2012-12-20

How to Cite

Voyda, Y. V., Biryukova, S. V., & Vysekantsev, I. P. (2012). Effect of Cryopreservation and Storage under Mineral Oil on Pheno- and Genotype Features of Escherichia coli Clinical Strains Isolated from Different Biotopes. Problems of Cryobiology and Cryomedicine, 22(3), 462–467. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/50

Issue

Vol. 22 No. 3 (2012): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 Yu. V. Voyda, S. V. Biryukova, I. P. Vysekantsev

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